中国对虾微卫星家系鉴定的模拟分析与应用
MICROSATELLITE MARKERS SIMULATION AND APPLICATION FOR PARENTAGE DETERMINATION ON FENNEROPENAEUS CHINENSIS
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摘要: 本研究基于中国对虾群体所获的微卫星标记等位基因频率进行了计算机模拟分析,并选择5个微卫星标记,就单独养殖家系群体微卫星标记家系鉴定的准确性及混养家系群体微卫星标记家系鉴定的应用价值做了研究。模拟分析表明4个微卫星标记可以鉴定95%的后裔。而单独养殖的家系鉴定准确率达到92.9%,在30个可能的父母对,215尾中国对虾组成的混养家系群体中,90.7%的后裔可以鉴定其父母。本研究结果表明微卫星分子标记可以应用于中国对虾的家系鉴定。模拟分析与实际应用的差异及父母与子代间的错配部分原因是由于无效等位基因的出现,基因分型错误也是一个重要原因。基于父母LOD值的分析可以降低错配的几率。Abstract: This study was initiated to assess the feasibility of parentage determination in aquaculture populations for Chineseshrimp ( Fenneropenaeus chinensis). Under different scenarios both simulation and reality, microsatellite markers were used to as-sign parentage to offspring. Simulations based on allele frequency data fromthe population of Chinese shrimp demonstrated that atleast 4 loci were required to assign over 95 % of progeny. When marker data fromfive loci were combined, the assignment success of progeny to their true parental couple increased to 9219 % in parentage identification with known parental and filial information. Out of 215 offspring, 9017 % was assigned to their parental pairs exclusively in mixed families groups. This studyshowed that the pedigree of progeny in mixed families could be satisfactorily determined using as few as five microsatellite markerseven though the shrimp could have originated from 30 possible pairs. Discrepancies between the simulations and real data setswere considered to be largely due to the mismatches caused by scoring errors at microsatellite loci. Analysis for the value of LODwould reduce the error occurred in parentage determination on Chinese shrimp. Based on these results, it was concluded that theuse of a number of microsatellite markers represented a realistic and effective alternative to physical tagging in a selection programand it allowed the identification of parental effects on offspring performances from the early life stages.
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