高浓度CO2下苦草的生长和生理生化反应
GROWTH AND PHYSIO-BIOCHEMISTRY RESPONSES OF VALLISNERIA SPIRALIS L.TO CO2 ENRICHMENT
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摘要: 对沉水植物苦草(Vallisneria spiraslis L.)在高浓度CO2(1000μmol/mol)和对照浓度CO2(350μmol/mol)下的生长特征和生理生化指标进行了比较研究.在实验的早期阶段,从冬芽出苗的苦草幼株在高浓度CO2下生长明显加快,但由于后期生长逐渐放慢,其最终总生物量比对照组仅高出11.%.尽管高浓度CO2也促进了根的生物量的累积,但是由于苦草叶片生物量占总株生物量比例大,高浓度CO2下苦草生物量的增加主要反映为叶片生物量的增加.在实验后期阶段,高浓度CO2促进了苦草冬芽的形成.实验过程中,苦草的根叶生物量比(RLR)在高浓度和对照浓度CO2下均有所降低,二者之间无明显统计学差异.高浓度CO2下苦草叶片中叶绿素含量和可溶性蛋白质含量降低,而可溶性总糖含量明显增加.Abstract: Growth and physio biochemistry responses of Vallinseria spiralis L.were studied comparatively at ambient(about 350μmol mol-1 )and elevated(about 1000μmol mol-1 )CO2 concentration.Young plants under elevated CO2 grew faster than their counterparts in ambient CO2 at early stage.But as this superiority did not persist throughout the experiment,finally plants at CO2 enriched environment accumulated just 11.6% more total biomass than those at control.The total biomass advantage of the V.spiralis under elevated CO2 was primarily due to its leaf weight increment superiority over the control at the end of the experiment.More turions formed under elevated CO2.Root/leaf ratio trended to decrease under both elevated and ambient CO2,but no significant difference was detected.Under elevated CO2 chlorophyll and soluble protein content decreased,While total soluble sugar content increased significantly.
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Keywords:
- CO2 Enrichment /
- Vallisneria spiralis L. /
- Growth
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P,et al.Physiological and biochemical responses of the seedlings of four legume tree species to high CO2 concentration[J].Acta Phytoecologica Sinica, 1999,23(3):220-227.[林丰平,陈章和,陈兆平,高CO2浓度下豆科几种乔木幼苗的生理生化反应.植物生态学报,1999,23(3):220-227] Conway T J, Tans P, Waterman L S,et al.Atmospheric carbon dioxide measurements in remote global troposphere,198l-1984[J].Tellus,1988,40B:81-115[2] Keeling C D,Whorf T P,Wahlen M,et al.International extremes in the rate of rise of atmospheric carbon dioxide since 1980[J].Nature,1995,325:666-670[3] Goudriaan J.Interaction of ocean and biosphere in their transient response to increasing atmosphere CO2[J].Vegetario,1993,104/105:329-337[4] Frederick T S, Hilary A N.The effects of global climate change on seagrasses[J].Aquatic Botany,1999,63:169-196[5] Idso K E,Idso S B.Plant responses to atmospheric CO2 enrichment in the face of environment constraints:a review of the past 10 years research[J].Agric.For.Meteorol,1994,69∶153-203[6] Jiang G M,Han X G,Lin G H.Response of Plant growth to elevated[CO2]:A review of the chief methods and basic conclusions based on experiments in the external countries in past decade[J].Acta Phytoecologia Sinica,1997,21(6),489-502.[蒋高明,韩兴国,林光辉,大气CO2浓度升高对植物的直接影响--国外十余年来模拟实验研究之主要手段及基本结论.植物生态学报.1997∶ 21(6),489-502][7] Xia J R, Gao K S.Effects of high CO2 concentration on growth and photosynthesis of Spirulina maxima [J].Acta Hydrobiologica Sinica, 2001,25(5):474-480[夏建荣,高坤山,2001.高浓度CO2对极大螺旋藻生长和光合作用的影响.水生生物学报,25(5):474-480][8] Spenser W, Bowes G.Photosynthesis and growth of water hyacinth under CO2 enrichment [J].Plant physiology,1986,82:528-533[9] Mckee J,Richards A J.Variation in seed production and germinability in common reed(Phragmites australis)in Britain and France with respect to climate[J].New Phytol,1996,133:233-243[10] Thom R M.CO2-enrichment effects on eelgrass(Zostera marina L.)and bull kelp (Nereocystis luetkeana (Mert.)P.R.)[J].Wat.Air Soil pollut,1996,88:383-391[11] Zimmerman R C,Kohrs D G,Steller D L,et al.Impacts of CO2 enrichment on production and light requirements of eelgrass[J].Plant Physiology,1997,115:599-607[12] Yu D,Zeng Y B,Zhang H H,et al.Restoration of aquatic vegetation and its role in the ecological pool of natural resources in Lake Yunihu[J].Chinese Journal of Applied Ecology,1996,7(4):401-406.[于丹,曾一本,张汉华,1996.淤泥湖退化水生植被恢复及其在湖泊资源天然生态库中作用的研究.应用生态学报,7(4):401-406][13] Carpenter S R,Lodge D M.Effects of submersed macrophytes on ecosystem process[J].Aquatic Botany,1986,26:341-370[14] Liu J k.Ecological study of Donghu LakeⅡ[M].Beijing:Science Press.1995.[刘建康.东湖生态学研究(二).北京:科学出版社.1995][15] Chen H D and Chen X Y.Measurement of chlorophyll content of aquatic plants[J].Fisheries of Hubei,1998,1:26-37.[陈洪达,陈晓燕,1988,水生植物叶绿素含量的测定.湖北渔业,1988,1:26-37][16] Zhang Z L.A guide to experiments of plant physiology [M].Shanghai:Shanghai Education Press.1994.[张志良,1994.植物生理实验指导.上海:上海教育出版社][17] Bradford M M.A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,17:248-254[18] Radoglou K M and Jarvis P G.Effects of CO2 enrichment on four poplar clones.I.Growth and leaf anatomy[J].Annals of Botany,1990,65:617-626[19] Ryle G J A,Pawell C E and Veronica Tewson.Effect of elevated CO2 on the photosynthesis,respiration and growth of perennial ryegrass[J].Journal of Experimental Botany,1992,43(251):811-818[20] Pooter H.Interspecific variation in the growth response of plants to an elevated ambient CO2 concentration[J].Vegetatio,1993,104/105:77-97[21] Koch E W.Hydrodynamics,diffusion-boundary layers and photosynthesis of the seagrass Thalassia testudinum and Cymodocea nodosa[J].Mar Bio,1994,118:767-776[22] Lemon E R.CO2 and Plants:Response of plants to rising levels of atmospheric carbon dioxide[M].Boulder:Westview Press,1983,223-280[23] Chen D X,Coughenour M B,Eberts D,et al.Interactive effects of CO2 enrichment and temperature on the growth of dioecious Hydrilla verticilla [J].Environ.Exp.Bot, 1994,34:345-353[24] Lawlor D W,Mitchell R A C.The eflfects of increased CO2 on crop photosynthesis and productivity:a review of field studies[J].Plant,Cell and Environment,1991,14:807-818[25] Osborne C P,Drake B G,Laroche J,et al.Does long-term elevation of CO2 concentration increase photosynthesis in forest floor vegetation?[J]Plant Physiology, 1997,114:337-344[26] Arp W J,Drake B G.Increased photosynthetic capacity of Scirpus olney after 4 years of exposure to elevated CO2[J].Plant, cell and Environment,1991,14:1004-1008[27] Beer S,Rehnberg J.The acquisition of inorganic carbon by the seagrass Zostera marina [J].Aquatic Botany,1997,56(3-4):277-283[28] Madsen T V,Maberly S C,Bowes G.Photosynthetic acclimation of submersed angiosperms to CO2 and HCO 3[J].Aquatic Botany,1996,53:15-30[29] Rogens G S,Milham P J,Gilings M et al.Sink strength may be the key to growth and nitrogen responsse in N-deficient wheat at elevated CO2[J].Aust.J.Plant.Physiol.,1996,23:253-264[30] Kuehny J S,Peet M M,Nelson P V and Willits K H.Nutrient dilution starch in CO2-enriched chrysanthemum[J].Journal of Experimental Botany,1991,42:711-716[31] Lin F P,Chen Z H,Chen Z P,et al.Physiological and biochemical responses of the seedlings of four legume tree species to high CO2 concentration[J].Acta Phytoecologica Sinica, 1999,23(3):220-227.[林丰平,陈章和,陈兆平,高CO2浓度下豆科几种乔木幼苗的生理生化反应.植物生态学报,1999,23(3):220-227]
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