摘要:
研究采用酶解技术水解太平洋鳕鱼肉, 制备不同分子量组分的两种水解鱼蛋白产品(Fish protein hydrolysate,FPH-A 和FPH-B)。在牙鲆幼鱼高植物蛋白饲料配方中, 以水解鱼蛋白产物1.2%和3.7%两个梯度替代饲料中的鱼粉蛋白, 在室内流水养殖系统中进行了为期60d 的生长实验。研究了高植物蛋白饲料中添加水解鱼蛋白对肉食性鱼类牙鲆(Paralichthys olivaceus)生长性能和饲料利用的影响。结果表明, 添加3.7%的水解鱼蛋白显著促进了牙鲆幼鱼的生长, 特别是添加了富含低分子量组分的水解蛋白产品(FPH-A)后实验鱼的特定生长率最高。各实验处理组牙鲆摄食率没有显著差异。摄食添加3.7% FPH-A 的牙鲆鱼体粗蛋白含量显著高于对照鱼粉组。添加水解鱼蛋白显著提高了牙鲆幼鱼的蛋白质消化率、蛋白质效率和蛋白质沉积率,摄食3.7% FPH-A 实验鱼蛋白质消化率、蛋白质效率和蛋白沉积率最高, 显著高于其他各组。实验表明, 高植物蛋白饲料中添加低分子量组分的水解鱼蛋白可显著提高牙鲆幼鱼的生长和饲料蛋白利用。
关键词:
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水解鱼蛋白
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植物蛋白
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牙鲆
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生长
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饲料利用
Abstract:
In the last decade, the increasing demand, price and world supply fluctuations of fish meal has emphasized the need to look for alternative protein sources in aquafeeds. Increased use of protein of plant origin and better use of available marine protein sources are important issues in this respect. The total volumes of by-products from the seafood industry are significant and could become valuable ingredients in feed for carnivorous fish species. Processing of fish hydrolysate from the by-products is applicable and these products are relevant as ingredients for fish feed. The present experiment was carried out to evaluate fish protein hydrolysate as feed ingredient in high plant protein diets for Japanese flounder (Paralichthys olivaceus). Fish protein hydrolysate was produced from Pollack (Pollacbius pollacbius) by enzymatic treatment. The fish hydrolysate was size fractionated by filtration. Fish protein hydrolysate product A (FPH-A) was the permeate sample after filtration. Fish protein hydrolysate product B (FPH-B) was the non-filtrated sample. Japanese flounder juvenile were fed with diets containing 1.2% and 3.7% fish protein hydrolysate in a continuous flow system. The growth experiment lasted for 60 days. The results showed that fish protein hydrolysate improved the growth of Japanese flounder. Specific growth rates of fish fed with 3.7% FPH-A was the highest. There were no significant differences in feeding rate of flounder. Crude protein composition of fish fed with 3.7% FPH-A was significantly higher than fish fed with control diet (fish meal). Fish protein hydrolysate significantly improved protein digestibility, protein retention and protein utilization of flounder juvenile. Fish fed with 3.7% FPH-A had the highest protein digestibility, protein retention and protein utilization. In conclusion, small molecular weight compounds from fish protein hydrolysate may successfully be used as protein source in high plant protein diets for Japanese flounder in exchange of fish meal.
百度学术
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