大黄鱼快长相关微卫星标记的筛选与验证
SCREENING AND VERIFICATION OF MICROSATELLITE MARKERS CLOSELY RELATED WITH RAPID GROWTH TRAITS IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA
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摘要: 为了筛选与大黄鱼生长性状紧密相关的分子标记，并对这些标记的有效性进行鉴定，研究以大黄鱼家系和群体为材料，经标记筛选、两次验证共三个步骤，找到2个与大黄鱼生长性状紧密相关的微卫星标记：LYC0088和LYC0143，其中LYC0088与体高、体质量均极显著相关（P0.01），与体长显著相关（P0.05），LYC0143与体长和体质量显著相关（P0.05），与体高的相关性极显著（P0.01）。LYC0088与LYC0143位点的优势等位基因分别为E、A，优势等位基因型均为AB，优势等位基因型组合为AB/AB，两个位点呈加性效应。该结果为开展大黄鱼分子标记辅助育种提供了有价值的遗传标记。Abstract: To screen the molecular markers closely related with growth traits and identify the validity of these markers in large yellow croaker, a family and a population were used as material and three steps were included. The first step was makers screening. In this step, a family with 60 extreme individuals was used as genetic material. The second step was markers preliminary verification. In this step, a family with 120 random individuals was used as genetic material. The last step was markers verification once again. A population with 60 extreme individuals was used. Two microsatellite markers closely related with growth traits were obtained. LYC0088 was significantly correlated with body height, body weight (P0.01), and body length (P0.05). LYC0143 was significantly correlated with body length and body weight (P0.05), and body height (P0.01). For LYC0088 and LYC0143 loci, the advantage allele was E and A, respectively. The advantage genotypes were both AB. The advantage combination of genotype was AB/AB. These two loci presented additive effects. The results provide valuable genetic markers for conducting large yellow croaker molecular marker-assisted breeding.
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Keywords:
- Large yellow croaker /
- Microsatellites /
- Growth traits /
- Rapid growth marker
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[1] Fishery Bureau of the Ministry of Agriculture.China Fishery Statistical Yearbook 2011[M].Beijing: China Agriculture Press.2011,Ⅱ[农业部渔业局编制.2011中国渔业统计年鉴.北京: 中国农业出版社.2011,Ⅱ]
[2] The National Fisheries Technology Extension Station.Promotion Guide of New Aquatic Varieties in 2010[M].Beijing: China Agriculture Press.2010,82-100[全国水产技术推广总站.2010水产新品种推广指南.北京: 中国农业出版社.2010,82-100]
[3] Liu Y G.Aquatic DNA Molecular Marker Technology[M].Beijing: Science Press.2009,20-67[刘云国.水产生物DNA分子标记技术.北京: 科学出版社.2009,20-67]
[4] Bao B L,Wang Z Y,Zhang S C,et al.Aquaculture Genomics Technologies[M].Beijing: Chemical Industry Press.2011,1-75[鲍宝龙,王志勇,张士璀,等.水产基因组学技术.北京: 化学工业出版社.2011,1-75]
[5] Ye H.Construction of SSR linkage maps and QTL analysis for growth-related traits in the large yellow croaker[D].Doctoral dissertation.Hunan Agricultural University,Changsha.2010[叶华.大黄鱼SSR遗传连锁图谱的构建及生长相关性状的QTL定位.博士学位论文,湖南农业大学,长沙.2010]
[6] Liu X D,Zhao G T,Wang Z Y,et al.Parentage assignment and parental contribution analysis in large yellow croaker (Larimichthys crocea) using microsatellite markers[J].Current Zoology,2012,58(2): 244-249
[7] Liu X D,Zhao G T,Cai M Y,et al.Estimated genetic parameters for growth-related traits in large yellow croakerLarimichthys crocea using microsatellites to assign parentage[J].Journal of Fish Biology,2013,82(1): 34-41
[8] itten crab (Eriocheir sinensis)[J].Acta Hydrobiologica Sinica,2011,35(2): 197-202[吴滟,付春鹏,蒋速飞,等.中华绒螯蟹微卫星标记与生长性状相关性的初步分析.水生生物学报,2011, 35(2): 197-202. Wu Y,Fu C P,Jiang S F, et al.Preliminary studies on the correlation between microsatellite markers and growth traits in Chinese mitten crab (Eriocheir sinensis)[J].Acta Hydrobiologica Sinica,2011,35(2): 197-202[吴滟,付春鹏,蒋速飞,等.中华绒螯蟹微卫星标记与生长性状相关性的初步分析.水生生物学报,2011, 35(2): 197-202]
[9] Ye H,Ren P,Liu Y,et al.Isolation and genetic analysis of microsatellite markers forLarimichthys crocea[J].Acta Hydrobiologica Sinica,2012,36(6): 1156-1163[叶华,任鹏,刘洋,等.大黄鱼微卫星标记的开发及其遗传方式分析.水生生物学报,2012,36(6): 1156-1163]
[10] Fu H T,Wan S Q,Fu C P, et al.Screening of microsatellite markers associated with growth traits inMacrobrachium nipponense[J].Acta Hydrobiologica Sinica,2010,34(5): 1043-1048[傅洪拓,万山青,付春鹏,等.青虾生长性状相关的微卫星标记筛选.水生生物学报,2010,34(5): 1043-1048]
[11] Gao G Q,Chang Y M,Han Q X,et al.Screening of microsatellite markers associated with cold tolerance of large yellow croaker (Pseudosciaena crocea R.)[J]. Hereditas(Beijing),2010,32(3): 248-253[高国强,常玉梅,韩启霞,等.大黄鱼耐低温性状相关微卫星标记的筛选.遗传,2010,32(3): 248-253]
[12] Liu X D,Wei X J,Cai M Y,et al.The segregation patterns of microsatellite markers in an F1 family of large yellow croakerLarimichthys crocea and correlation analysis with growth-related traits[J].Journal of Fisheries of China,2012,36(9): 1322-1329[刘贤德,韦信键,蔡明夷,等.大黄鱼22个微卫星标记在F1家系中的分离方式及与生长性状的相关分析.水产学报,2012,36(9): 1322-1329]
[13] Wang X P,Zhang X F,Li W S,et al.Mapping and genetic effect analysis on quantitative trait loci related to feed conversion ratio of common carp (Cyprinus carpio L.)[J]. Acta Hydrobiologica Sinica,2012,36(2): 177-196[王宣朋,张晓峰,李文升,等.鲤饲料转化率性状的QTL定位及遗传效应分析.水生生物学报,2012,36(2): 177-196]
[14] Sun X W,Lu C Y,Cao D C,et al.Molecular markers associated with body weight of mirror carp and selection and raising of progenies[J]. Journal of Fisheries of China,2009,33(2): 77-81[孙效文,鲁翠云,曹顶臣,等.镜鲤体重相关分子标记与优良子代的筛选和培育.水产学报,2009,33(2): 77-81]
[15] Wang H Z,Zhang Y,Yin Q Q, et al.Analysis of genetic diversity and economic traits in two mirror carp semi-sib families[J].Acta Hydrobiologica Sinica,2009,33(3): 522-531[王洪哲,张研,殷倩茜,等.两个镜鲤半同胞家系的遗传多样性及经济性状分析.水生生物学报,2009,33(3): 522-531]
[16] Xu K,Ma A J,Wang X A,et al.Microsatellites molecular markers and the correlation to growth trait ofScophthalmus maximus[J].Oceanologia et Limnologia Sinica,2009,40(5): 577-583[许可,马爱军,王新安,等.大菱鲆(Scophthalmus maximus)生长性状相关的微卫星标记筛选,海洋与湖沼,2009,40(5): 577-583]
[17] Xue L Y,Sun S,Xiao Z K,et al.Microsatellite polymorphism analysis of myostatin fromPseudosciaena crocea[J].Chinese Journal of Biochemistry and Molecular Biology,2008,24(10): 980-985[薛良义,孙升,肖章奎,等.大黄鱼肌肉生长抑制素基因微卫星序列多态性分析.中国生物化学与分子生物学报,2008,24(10): 980-985]
[18] Yang J,Zhang X F,Chu Z Y,et al.Correlation analysis of microsatellite markers with body weight,length,height and upper jaw length wensize of common carp (Cyprinus carpio L.)[J]. Journal of Fishery Sciences of China,2010,17(4): 721-730[杨晶,张晓峰,储志远,等.鲤的微卫星标记与体质量、体长、体高和吻长的相关分析.中国水产科学,2010,17(4): 721-730]
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