微颗粒饲料中添加精胺对半滑舌鳎稚鱼生长和肠道发育的影响
THE EFFECT OF MICRODIET SUPPLEMENTATION WITH SPERMINE ON THE GROWTH AND INTESTINAL DEVELOPMENT OF TONGUE SOLE POSTLARVAE
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摘要: 为获知微颗粒饲料中添加精胺对半滑舌鳎仔稚鱼肠道发育的影响，试验以添加0，0.10%，0.33%精胺的微颗粒饲料（F1、F2、F3）和活饵料卤虫（F4）饲喂初始体长为2 cm左右的半滑舌鳎稚鱼。养殖28d后结果表明，卤虫组（F4）的特定生长率最高，饲料组中F2组特定生长率要显著高于F1和F3组（P0.05）。F3组的存活率仅为60.27%，显著低于其他各组（P0.05）。消化酶活力测定结果中，F2组在14d和28d时都含有较高的碱性磷酸酶比活力和亮氨酸氨基肽酶比活力，较低的亮氨酸-丙氨酸肽酶比活力。卤虫组的肠道发育情况最好，微绒毛长度显著大于其他各组（P0.05）；黏膜厚度略小于F2组，但是没有显著性差异（P0.05）；饲料组中F2组微绒毛长度和黏膜厚度都显著大于F1和F3组（P0.05）。研究表明，在微颗粒饲料中添加0.10%的精胺（F2组）对半滑舌鳎稚鱼肠道发育有促进作用。Abstract: To study the effects of spermine on the intestinal development of Cynoglossus semilaevis postlarvae, we selected the subject with initial length of about 2 cm and fed them with microdiets containing 0 (F1), 0.10% (F2), 0.33% (F3) of spermine and live Artemia (F4) for 28 days. The results showed that F4 group exhibited the highest growth and survival rates. The specific growth rate of F2 group was significantly higher than that of F1 and F3 groups (P0.05). The survival rate of F3 group was 60.27%, which was significantly lower than that of other groups (P0.05). At day 14, the alkaline phasphatase relative activities (AP) in the bodies of fish fed with spermine-containing microdiet were significantly higher than that in fish fed with the Artemia (P0.05); there was no significant difference among groups fed with spermine-containg mecrodiets (P0.05). At day 28, the AP of fish fed with 0.10% spermine was significantly higher than other groups (P0.05); no significant difference was found among other groups (P0.05). F2 and F3 groups showed higher leucine aminopeptidase (LAP) specific activities than F1 and F4 groups at day 14 and 28 (P0.05), but there was no significant difference between F2 and F3 (P0.05). F2 group showed the lowest leucine-alanine peptidase (leuala) specific activity at day 14 and 28 (P0.05). The microvilli length of Artemia group was significantly greater than that of the other groups (P0.05). The mucosal layer of Artemia group was apparently but insignificantly hinner than that of F2 group (P0.05). Both the length of microvilli and the thickness of the mucosal layer in F2 were greater than those of F1 and F3 groups (P0.05). These results suggested that 0.10% spermine in microdiet had positive effects on the intestinal development of tongue sole postlarvae.
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Keywords:
- Microdiet /
- Spermine /
- Cynoglossus semilaevis /
- Postlarvae /
- Intestinal development
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[1] Liu X Z, Zhuang Z M, Ma A J, et al. Operative technologies for seedling rearing of Cynoglossus semilaevis Gnter [J]. Marine Fisheries Research, 2006, (2): 1724 [柳学周, 庄志猛, 马爱军, 等. 半滑舌鳎苗种生产技术的开发研究. 海洋水产研究, 2006, (2): 1724]
[2] Cahu C L, Infante J Z. Maturation of the pancreatic and intestinal digestive functions in sea bass (Dicentrarchus labrax): effect of weaning with different protein sources [J]. Fish Physiology and Biochemistry, 1995, 14(6): 431437
[3] Chang Q, Cheng S Q, Zhang X M, et al. Histological study of the organogenesis of the digestive system of the tonguefish, Cynoglossus semilaevis [J]. Journal of Fisheries of China, 2006, 29(4): 447453 [常青, 陈四清, 张秀梅, 等. 半滑舌鳎消化系统器官发生的组织学. 水产学报, 2006, 29(4): 447453]
[4] Thomas T, Thomas T J. Polyamines in cell growth and cell death: molecular mechanisms and therapeutic applications [J]. Cellular and Molecular Life Sciences, 2001, 58(2): 244258
[5] Uda K, Tsujikawa T, Fujiyama Y, et al. Rapid absorption of luminal polyamines in a rat small intestine ex vivo model [J]. Journal of Gastroenterology and Herpetology, 2003, 18(5): 554559
[6] Peulen O, Deloyer P, Dandrifosse G. Short-term effects of spermine ingestion on the small intestine: a comparison of suckling and weaned rats [J]. Reproduction Nutrition Development, 2004, 44(4): 353364
[7] Osborne D L, Seidel E R. Microflora-derived polyamines modulate obstruction-induced colonic mucosal hypertrophy [J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 1989, 256(6): G1049G1057
[8] Wild G E, Daly A S, Sauriol N, et al. Effect of exogenously administered polyamine on the structural maturation and enzyme ontogeny of the postnatal rat intestine [J]. Neonatology, 1993, 63(4): 246257
[9] Peulen O, Pirlet C, Klimek M, et al. Comparison between the natural postnatal maturation and the spermine-induced maturation of the rat intestine [J]. Archives of Physiology and Biochemistry, 1998, 106(1): 4655
[10] Pres A, Cahu C L, Zambonino Infante J L. Dietary spermine supplementation induces intestinal maturation in sea bass (Dicentrarchus labrax) larvae [J]. Fish Physiology and Biochemistry, 1997, 16(6): 479485
[11] Crane R K, Boge G, Rigal A. Isolation of brush border membranes in vesicular form from the intestinal spiral valve of the small dogfish (Scyliorhinus canicula) [J]. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1979, 554(1): 264267
[12] Maroux S, Louvard D, Barath J. The aminopeptidase from hog intestinal brush border [J]. Biochimica et Biophysica Acta (BBA)-Enzymology, 1973, 321(1): 282295
[13] Nicholson J A, Kim Y S. A one-step L-amino acid oxidase assay for intestinal peptide hydrolase activity [J]. Analytical Biochemistry, 1975, 63(1): 110117
[14] Sousadias M G, Smith T K. Toxicity and growth-promoting potential of spermine when fed to chicks [J]. Journal of Animal Science, 1995, 73(8): 2375-2381
[15] Liu X D, Liang M Q, Zhang L M, et al. Effect of fish protein hydrolysate levels on growth performance and biological and physiological parameters in tongue sole (Cynoglossus semilaevis Gnter, 1873) [J]. Acta Hydrobiologica Sinica, 2010, 34(2): 242249 [柳旭东, 梁萌青, 张利民, 等. 饲料中添加水解蛋白对半滑舌鳎稚鱼生长及生理生化指标的影响. 水生生物学报, 2010, 34(2): 242249]
[16] Tong X H, Xu S H, Liu Q H, et al. Digestive enzyme activities of turbot (Scophthalmus maximus L.) during early developmental stages under culture condition [J]. Fish Physiology and Biochemistry, 2012, 38(3): 715724
[17] Infante J L Z, Cahu C. Development and response to a diet change of some digestive enzymes in sea bass (Dicentrarchus labrax) larvae [J]. Fish Physiology and Biochemistry, 1994, 12(5): 399408
[18] Garca Hernndez M P, Lozano M T, Elbal M T, et al. Development of the digestive tract of sea bass (Dicentrarchus labrax L.). Light and electron microscopic studies [J]. Anatomy and Embryology, 2001, 204(1): 3957
[19] Ortiz-Delgado J B, Darias M J, Caavate J P, et al. Organogenesis of the digestive tract in the white seabream, Diplodus sargus [J]. Histological and Histochemical Approaches, 2003, 18(4): 11411154
[20] Kvle A, Mangor-Jensen A, Moren M, et al. Development and characterisation of some intestinal enzymes in Atlantic cod (Gadus morhua L.) and Atlantic halibut (Hippoglossus hippoglossus L.) larvae [J]. Aquaculture, 2007, 264(1): 457468
[21] Cuvier-Pres A, Kestemont P. Development of some digestive enzymes in Eurasian perch larvae Perca fluviatilis [J]. Fish Physiology and Biochemistry, 2001, 24(4): 279285
[22] Murai T. Leucine aminopeptidase (LAP)] [J]. Rinsho Byori, 2001, 116(2): 110116
[23] Henning S J. Functional Development of the Gastrointestinal Tract [M]. In: Johnson L R (Eds.), Physiology of the Gastrointestinal Tract. New York: Raven Press. 1987, 285300
[24] Ji H, Sun H T, Tian J J, et al. Digestive enzyme activity during early larval development of the paddlefish Polyodon spathula [J]. Acta Hydrobiologica Sinica, 2012, 36(3): 457465 [吉红, 孙海涛, 田晶晶, 等. 匙吻鲟仔稚鱼消化酶发育的研究. 水生生物学报, 2012, 36(3): 457465]
[25] Nicholson J A, McCarthy D M, Kim Y S. The responses of rat intestinal brush border and cytosol peptide hydrolase activities to variation in dietary protein content dietary regulation of intestivial peptide hydrolases [J]. Journal of Clinical Investigation, 1974, 54(4): 890898
[26] Ribeiro L, Zambonino-Infante J L, Cahu C, et al. Development of digestive enzymes in larvae of Solea senegalensis, Kaup 1858 [J]. Aquaculture, 1999, 179(1): 465473
[27] Suzer C, Aktln S, oban D, et al. Digestive enzyme activities in larvae of sharpsnout seabream (Diplodus puntazzo) [J]. Comparative Biochemistry and PhysiologyPart A: Molecular Integrative Physiology, 2007, 148(2): 470477
[28] Zouiten D, Ben Khemis I, Slaheddin Masmoudi A, et al. Comparison of growth, digestive system maturation and skeletal development in sea bass larvae reared in an intensive or a mesocosm system [J]. Aquaculture Research, 2011, 42(11): 17231736
[29] Boglino A, Darias M J, Ortiz-Delgado J B, et al. Commercial products for Artemia enrichment affect growth performance, digestive system maturation, ossification and incidence of skeletal deformities in Senegalese sole (Solea senegalensis) larvae [J]. Aquaculture, 2012, 324(12): 290302
[30] Peulen O, Deloyer P, Grandfils C, et al. Intestinal maturation induced by spermine in young animals [J]. Livestock Production Science, 2000, 66(2): 109120
[31] Wang M, Hou Y Q, Ding B Y, et al. Effect of spermine on histological morphology and function of small intestine in weaned piglets [J]. Chinese Journal of Animal Nutrition, 2007, 19(4): 366371 [王猛, 侯永清, 丁斌鹰, 等. 精胺对断奶仔猪小肠组织形态的影响. 动物营养学报, 2007, 19(4): 366371]
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