摄食不同淀粉含量饲料对军曹鱼血清生化指标的影响
Effects of dietary starch on biochemical indexes of the serum of cobia (Rachycentron canadum)
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摘要: 实验旨在研究经过10周投喂不同淀粉含量的饲料的养殖实验后, 禁食1d后再投喂对170 g军曹鱼血清生化指标的影响. 饲料以鱼粉为蛋白源, 鱼油、豆油、大豆卵磷脂为脂肪源, 分别添加0%(对照组)、6%、12%、18%、24%和30%的小麦淀粉(以微晶纤维素调平), 养殖实验期间每天饱食投喂2次. 结果表明, 饥饿再投喂饲料后各处理组军曹鱼血糖含量均在(0-5)h升高, 在5h时达到峰值, 并显著高于其他时间组, 摄食5h后开始下降, 7h时基本恢复到投喂前水平; 血清总蛋白含量在不同处理组以及同一处理组的不同时间点上均没有显著差异; 血清中甘油三酯含量在(0-5)h显著升高, 5h后其含量开始下降, 7h时基本恢复到投喂前水平. 其中0%、6%和12%组, 摄食5h后其甘油三酯含量显著高于摄食前与摄食后7h和24h, 而在18%、24%和30%组中, 其含量无显著差异; 0%淀粉组血清中胆固醇含量在(0-2)h显著升高, 2h时达到峰值, 在(2-7)h呈下降趋势, 之后趋于平稳. 其他处理组都是在(0-5)h升高, 5h后开始下降, 在7h时基本恢复到投喂前水平. 18%-30%组, 摄食后5h血清中胆固醇含量显著高于摄食前与摄食后24h. 军曹鱼血清中低密度脂蛋白-胆固醇在(0-5)h显著升高, 摄食5h后其含量开始下降, 7h时基本恢复到投喂前水平, 但各组不同时间点间均无显著性差异. 而血清中高密度脂蛋白-胆固醇含量除在24%添加组中, 摄食后5h显著高于摄食前(0h)及投喂结束后24h处理组, 其他处理之间无显著差异. 综上所述: 170 g军曹鱼禁食再摄入不同含量的小麦淀粉后其血清中血糖含量先升高后降低, 随着饲料中糖添加量的增加, 其峰值先增加, 后趋于平稳. 军曹鱼血清甘油三酯和胆固醇的含量会升高, 对其血清中低密度脂蛋白-胆固醇及高密度脂蛋白-胆固醇影响不显著.Abstract: To investigate effects of re-feeding following fasting on plasma glucose, total protein, triacylglycerol, total cholesterol, low-density lipoprotein-cholesterol (LDL-C) and high-density lipoprotein-cholesterol (HDL-C) contents in cobia fasted for 24h, six diets were formulated to contain 0% (control), 6%, 12%, 18%, 24% and 30% starch, respectively. The results showed that the serum glucose levels of cobia after re-feeding with each diet elevated from 0h to 5h and then started to decline till 7h that the glucose levels were similar to those at 0h. Different dietary starch and postprandial time did not significantly influence the total protein levels in serum. The triacylglycerol levels in serum elevated from 0h to 5h and then began to decline. At 7h, the levels were similar to those at 0h. In the control group, the concentration of cholesterol in serum increased from 0h to 2h and then decreased from 2h to 7h that reached to fasting level. In other groups, the serum cholesterol levels increased from 0h to 5h and then began to decline; then, it also reached to fasting level at 7h. The levels of LDL-C increased from 0h to 5h and then began to decline. At 7h, the levels of LDL-C declined to the basal level. However, different dietary starch and postprandial time did not significantly influence the levels of LDL-C in serum. In the group of 24%, the levels of HDL-C at 5h were significantly higher than those at 0 and 24h. No significant differences were found for the levels of HDL-C among other groups. In conclusion, the glucose levels increased and then declined. The peak glucose levels increased with the increase of dietary starch level up to 12% and remained nearly the same thereafter. The levels of triacylglycerol and cholesterol in serum increased after re-feeding, but there were no significant differences in the LDL-C and HDL-C levels in serum after re-feeding
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Keywords:
- Cobia (Rachycentron canadum) /
- Wheat starch /
- Glucose /
- Re-feeding after fasting
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[1] Dixon D G, Hilton J W. Influence of available dietary carbohydrate content on tolerance of waterborne copper by rainbow trout, Salmo gairdneri Richardson [J]. Journal of Fish Biology, 1981, 19(5): 509-517
[2] Wang J Y, Zhu S G, Xu C F, Biochemistry (3rd edition) [M]. Beijing: Higher Education Press. 2002, 147-196, 230-298 [王镜岩, 朱胜庚, 徐长法. 生物化学(第三版)(下册). 北京: 高等教育出版社. 2002, 147-196, 230-298]
[3] Han T, Wang J T, Wang Y, et al. Effect of different fish protein hydrolysate (FPH) level of dietary supplements on growth and body composition of larvae of cobia (Rachycentron canadum) [J]. Acta Hydrobiologica Sinica, 2010, 34(1): 94-99 [韩涛, 王骥腾, 王勇, 等. 饲料中不同水平鱼蛋白水解物对军曹鱼至于生长组成及体组成的影响. 水生生物学报, 2010, 34(1): 94-99]
[4] Li L D, Chen B S, Feng J, et al. Analysis and evaluation in nutritive value of Rachycentron canadum (Linnaeus) [J]. Journal of Tropical Oceanography, 2002, 21(1): 76-82. [李刘冬, 陈毕生, 冯娟, 等. 军曹鱼营养成分的分析及评价. 热带海洋学报, 2002, 21(1): 76-82]
[5] Cui X J, Zhou Q C, Liang H O, et al. Effects of dietary carbohydrate sources on the growth performance and hepatic carbohydrate metabolic enzyme activities of juvenile cobia (Rachycentron canadum Linnaeus) [J]. Aquaculture Research, 2010, 42: 99-107
[6] Ren M C. Studies on nutritional physiology of carbohydrate for cobia and rainbow trout [D]. Thesis for Doctor of Science. Ocean University of China, Qingdao. 2012 [任鸣春. 军曹鱼和虹鳟糖类生理研究. 博士学位论文, 中国海洋大学, 青岛. 2012]
[7] Cui X J. Study on mechanism of carbohydrate utilization for juvenile cobia, Rachycentron canadum [D]. Thesis for Master of Science. Guangdong Ocean University, Zhanjiang. 2010 [崔现军, 军曹鱼糖代谢机理的研究. 硕士学位论文, 广东海洋大学, 湛江. 2010]
[8] Ren M C, Ai Q H, Mai K S, et al. Effect of dietary carbohydrate level on growth performance, body composition, apparent digestibility coefficient and digestive enzyme activities of juvenile cobia, Rachycentron canadum L [J]. Aquaculture Research, 2011, 42: 1467-1475
[9] Association of Official Analytical Chemists, Official Method Analysis, 16th edn [M]. Association of Official Analytical Chemists. Arlington, VA, USA. 1995, 69-78
[10] Barham Denise, Trinder P. An improved colour reagent for the determination of blood glucose by the oxidase system [J]. Analyst, 1972, 97: 142
[11] Peters T. Proposals for standardisation of total protein assays [J]. Clinical Chemistry, 1968, 14(12): 1147-1159
[12] Richmond W. Preparation and properties of a cholesterol oxidase from Norcardiu sp. and its application to the enzymatic assay of total cholesterol in serum [J]. Clinical Chemistry, 1973, 19: 1350-1356
[13] Schettler G. Nussel E: Determination of triglycerides [J]. Arbeitsmed Sozialmed Praventivmed, 1975, 10: 10-25.
[14] Gordon T, Castelli Wp, Hjortland Mc, et al. High density lipoprotein as a protective factor against coronary heart disease [J]. The American Journal of Medicine, 1977, 62: 707-714
[15] Okada M, Matsui H, Ito Y, et al. Low-density lipoprotein cholesterol can be chemically measured: a new superior method [J]. The Journal of Laboratory and Clinical Medicine, 1998, 132: 195-201
[16] Wang F, Tan Q S, Xie S Q, et al. Metabolic responses of plasma glucose and triglyceride of gibel carp (Carassiusauratus gibelio) after oral administration of different dosage of soluble starch [J]. Acta Hydrobiologica Sinica, 2008, 32(4): 610-614 [王芬, 谭青松, 解绶启, 等. 异育银鲫口服不同剂量淀粉后血糖和血脂代谢变化. 水生生物学报, 2008, 32(4): 610-614]
[17] Liu H L, Sun M M, Wang H W, et al. Effects of glucose injection on biochemical parameters, insulin, andglycolytic enzymes in GIFT (Oreochromis niloticus) [J]. Journal of Fishery Sciences of China, 2012, 19(5): 813-820 [刘含亮, 孙敏敏, 王红卫, 等. 注射葡萄糖对吉富罗非鱼血浆生化指标、胰岛素和糖酵解关键酶的影响. 中国水产科学, 2012, 19(5): 813-820]
[18] Cai C F, Liu Y, Chen L Q, et al. Metaboltic responses of allogyno genetic gibel carp after oral administration of different doses of glucose [J]. Acta Hydrobiologica Sinica, 2003, 27(6): 602-606 [蔡春芳, 刘影, 陈立侨, 等. 异育银鲫口服不同剂量葡萄糖后的代谢反应. 水生生物学报, 2003, 27(6): 602-606]
[19] Tian L X, Liu Y J, Liu D H, et al. Studies on the utilization of glucose and starch as an energy source of grass carp [J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2001, 3, 40(2): 104-106 [田丽霞, 刘永坚, 刘栋辉, 等. 草鱼对葡萄糖和淀粉作为能源的利用研究. 中山大学学报(自然科学版), 2001, 3, 40(2): 104-106]
[20] Lin X Z, Luo Y P, Xie X J. Effects of dietary carbohydrate level on glycolytic enzymes and serum glucose concentrations in the juvenile southern catfish after feeding [J]. Acta Hydrobiologica Sinica, 2006, 30(3): 304-310 [林小植, 罗毅平, 谢小军. 饲料碳水化合物水平对南方鲇幼鱼餐后糖酵解酶活性及血糖浓度的影响. 水生生物学报, 2006, 30(3): 304-310]
[21] Suarez M D. Metabolic effects of changes in the dietary protein: carbohydrate ratio in eel (Angillaanguilla) and trout (Oncorhynchus mykiss) [J]. Aquaculture International, 2002, 10(2): 143-156
[22] Yang W, Ye J D, Wang K, et al. Glucose tolerance in grouper (Epinphelus coioides) [J]. Acta Hydrobiologica Sinica, 2012, 36(3): 563-568 [杨伟, 叶继丹, 王琨, 等. 斜带石斑鱼经葡萄糖灌喂后的代谢反应. 水生生物学报, 2012, 36(3): 563-568]
[23] Deng D F, Refstie S, Hung S S O. Glycemic and glycosuric responses in white sturgeon (Acipenser transmontanus) after oral administration of simple and complex carbohydrates [J]. Aquaculture, 2001, 199: 107-117
[24] Peres H, Goncalves P, Oliva-Teles A. Glucose tolerance in gilthead sea bream (Sparusaurata) and European sea bass (Dicentrarchus labrax) [J]. Aquaculture, 1999, 179: 415-423
[25] Palmer T N, Ryman B E. Studies on oral glucose intolerance in fish [J]. Journal of Fish Biology, 1972, 4: 311-319
[26] Sun L, Wang J J, Zhuang Y Y. Modify low density lipoprotein by lipid transfer activity and triglyceride hydrolysis [J]. Basic Medical Sciences and Clinics, 1999, 19(4): 51-55. [孙林, 王俊军, 庄一义. 脂质转运和酯酶水解对低密度脂蛋白的修饰作用. 基础医学与临床, 1999, 19(4): 51-55]
[27] Zhao S P. Advance of the studies on high density lipoprotein [J]. Chinese Journal of Arteriosclerosis, 2005, 13(6): 673-675 [赵水平. 高密度脂蛋白的研究现状. 中国动脉硬化杂志, 2005, 13(6): 673-675]
[28] Yin X J, Ye Y T, Cai C F, et al. The effects of corn, wheat and cassava on plasma glucose and lipid levels of Ctenopharyngodon idella juveniles after feeding [J]. China Feed, 2010, 9: 18-22 [尹晓静, 叶元土, 蔡春芳, 等. 玉米、小麦、木薯日粮对草鱼幼鱼餐后血糖、血脂水平的影响. 中国饲料, 2010, 9: 18-22]
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