正常和蛙病毒感染后大鲵血清和黏液蛋白图谱比较分析
COMPARATIVE ANALYSIS OF SERUM AND SKIN MUCUS PROTEIN PROFILES BETWEEN RANAVIRUS-INFECTUD AND NORMAL CHINESE GIANT SALAMANDER ANDRIAS DAVIDIANUS
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摘要: 为了更好地了解大鲵在感染蛙病毒过程中的生理生化及抗病毒反应, 通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳(SDS-PAGE) 辅以密度分析, 对大鲵正常血清和感染血清、正常黏液和感染黏液的蛋白图谱分别进行测试比较。结果显示: 在正常血清(CS)、包括自然感染血清(NS)和人工感染血清(AS)在内的感染血清中,蛋白带集中在6080 kD, 其含量超过血清蛋白总量的42%; 正常血清在1457 kD 之间有16条蛋白带: 57、53、50、43、40、38、36、31、27、26、25、22、16、15.5、15 和14 kD, 其中正常与感染血清(包括NS和AS)比较, 有10条共有蛋白带: 57、43、40、31、27、26、25、15.5、15 和14 kD和4条差异蛋白带: 53、33、22 和16 kD。正常黏液(CM)和感染黏液(NM或AM)共有11条蛋白带: 116、100、75、57、53、45、27、18、17、16和15 kD, 但感染黏液多出7 条蛋白带: 90、52、43、32、26、20 和13 kD。还有些蛋白带含量出现变化, 如45 kD条带在CM中占总蛋白量的19.3%, 而在AM中仅占总蛋白的3.8%。研究证实蛙病毒感染能导致大鲵黏液和血清蛋白图谱发生变化, 也为深入探寻两栖类抗病毒相关的蛋白生物标记提供了有价值的信息。Abstract: The Chinese giant salam Andrias davidianus isendemicto China and is the largest remaining amphibian in the world, which has considerable economic and scientific significance. However, epidemic of ranavirus disease have been responsible forthe wild population continued to decline a sharp drop in giant salamander production. For better understanding of the physiological and biochemical reactions, and antiviral responses of Chinese giant salamander, the protein profiles of serum and mucus from control and infected giant salamanders were analysized and compared by sodium dodecyl sulfate polyacrilamide gel electrophoresis(SDS-PAGE) and the densitometric analysis. The results show that protein bands from control serum (CS) and infected serum (including natural infected serum, NS and artificial infection serum, AS) were enriched at 6080 kD, the contents were more than 42% of total protein. CS has 16 protein bands between 1457 kD. Besides, sixteen protein bandswere present among 1457 kD in CS: 57, 53, 50, 43, 40, 38, 36, 31, 27, 26, 25, 22, 16, 15.5, 15 and 14kD. Fourprotein bands 53, 33, 22 and 16 kD were different between CS and NS, AS. Control mucus (CM) and infected mucus (including n atural infection mucus, NM and artificial infection mucus, AM) have eleven common protein bands 116, 100, 75, 57, 53, 45, 27, 18, 17, 16 and 15 kD. But infected giant salamander mucus NM and AM have seven other protein bands 90, 52, 43, 32, 26, 20 and 13 kD. Furthermore, the protein contents of some bands have changed, for example, the 45 kD band in CM makes up 19.3% of total protein. However, it only makes up 3.8% in AM. This study has demonstrated that ranavirus infection can lead to protein components change in Chinese giant salamanders mucus and serum, which provided valuable information for explore antiviral associated protein biomarkers in amphibians.
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