POLYMORPHISM OF MSTN GENE AND ITS ASSOCIATION WITH GROWTH TRAITS IN CHINESE MITTEN CRAB, ERIOCHEIR SINENSIS
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摘要: 为研究中华绒螯蟹(Eriocheir sinensis)肌肉生长抑制素基因(myostatin, MSTN)的多态性及其与生长性状的相关性, 对中华绒螯蟹3个群体(育种群体、大赛群体、野生群体)共321个个体MSTN基因的多态性进行筛选, 发现该基因的第1外显子存在3个多态性SNP位点(S1: C714T; S2:G729A; S3:G753T), 均为处于Hardy-Weinberg平衡(P>0.05)的中、高度多态性位点。利用一般线性模型分析3个位点及其基因型组合与生长性状的相关性, 发现S1位点对中华绒螯蟹的体重和壳长等生长性状有显著影响(P≤0.05), 而其余2个位点与生长性状无显著关联性。结果表明S1位点的TT基因型对中华绒螯蟹的生长最为有利, 可作为分子标记辅助育种的候选标记。Abstract: To study the polymorphism of myostatin (MSTN) gene and its association with growth traits in Chinese mitten crab Eriocheir sinensis, 321 individuals from the three populations (breeding population, contest population and wild population) were screened polymorphic sites of MSTN gene. Three SNPs were found in the first exon of MSTN gene, which were named as S1, S2 and S3, respectively (S1:C714T; S2:G729A; S3:G753T). These three SNPs were middle or high polymorphism loci, and they were all in hardy-Weinberg equilibrium (P>0.05) in population structure. A general linear model used to analyze the correlation between the three SNPs and growth traits (body weight and carapace length) of Chinese mitten crab indicated that S1 but not S2 and S3 had a significant effect on the growth traits (P≤0.05). The TT genotype was the most beneficial genotype on growth of Chinese mitten crab, which could be used as candidate for molecule marker-assisted breeding.
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表 1 中华绒螯蟹三群体MSTN基因3个SNP位点的遗传参数统计
Table 1 Genetic parameters of three SNP sites in the MSTN gene from three populations of Chinese mitten crab
群体
PopulationSNP位点
Locus观测杂合度
Ho期望杂合度
He有效等位基因数
Ne多态信息含量
PICHardy-Weinberg平衡
PHWE育种群体
Breeding populationS1 0.4583 0.4752 1.976 0.372 0.4563 S2 0.5001 0.4104 1.683 0.3236 0.3346 S3 0.4917 0.4325 1.753 0.3374 0.5526 大赛群体
Contest populationS1 0.4424 0.3942 1.641 0.5794 0.8712 S2 0.3876 0.3578 1.5585 0.5439 0.4589 S3 0.4355 0.412 1.7106 0.606 1.0673 野生群体
Wild populationS1 0.3618 0.4633 1.8345 0.6254 0.9548 S2 0.432 0.3973 1.6984 0.584 0.4312 S3 0.4198 0.4099 1.8542 0.433 0.8738 总群体
Total populationS1 0.4175 0.4031 1.7634 0.5433 0.3427 S2 0.4509 0.3988 1.6128 0.5347 0.0762 S3 0.4432 0.4156 1.7893 0.4723 0.5692 
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表 2 中华绒螯蟹三个群体MSTN基因SNP位点的等位基因及基因型频率
Table 2 Genotype and allele frequency of three SNP sites in the MSTN gene from the three populations of Chinese mitten crab
群体
Populations位点
Locus样品数
Number基因型频率
Genotype frequency等位基因频率
Allele frequencyχ2检验
χ2-testingP value 育种群体
Breeding
populationS1(T/C) 120 TT/0.33(n=39) TC/0.46(n=55) CC/0.22(n=26) T/0.55 C/0.45 0.6298 0.4474 S2(A/G) 120 AA/0.47(n=56) AG/0.50(n=60) GG/0.03(n=4) A/0.72 G/0.28 2.4139 0.0713 S3(T/G) 120 TT/0.44(n=53) TG/0.49(n=59) GG/0.07(n=8) T/0.69 G/0.31 2.4967 0.1141 大赛群体
Contest
populationS1(T/C) 124 TT/0.52(n=64) TC/0.44(n=54) CC/0.04(n=6) T/0.73 C/0.27 0.7760 0.2008 S2(A/G) 124 AA/0.57(n=71) AG/0.39(n=48) GG/0.04(n=5) A/0.77 G/0.23 0.7979 0.3717 S3(T/G) 124 TT/0.48(n=59) TG/0.45(n=56) GG/0.07(n=9) T/0.70 G/0.30 0.7660 0.3815 野生群体
Wild
populationS1(T/C) 77 TT/0.49(n=38) TC/0.44(n=34) CC/0.06(n=5) T/0.71 C/0.29 0.5146 0.4728 S2(A/G) 77 AA/0.55(n=42) AG/0.40(n=31) GG/0.06(n=4) A/0.75 G/0.25 0.3197 0.5718 S3(T/G) 77 TT/0.52(n=40) TG/0.36(n=28) GG/0.12(n=9) T/0.70 G/0.30 1.3426 0.2466 总群体
Total
populationS1(T/C) 321 TT/0.44(n=141) TC/0.45(n=143) CC/0.12(n=37) T/0.66 C/0.34 0.5066 0.9351 S2(A/G) 321 AA/0.53(n=169) AG/0.43(n=139) GG/0.04(n=13) A/0.74 G/0.26 2.7492 0.0865 S3(T/G) 321 TT/0.47(n=152) TG/0.45(n=143) GG/0.08(n=26) T/0.70 G/0.30 0.9100 0.3401
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表 3 中华绒螯蟹三群体MSTN基因的SNP位点基因型与生长性状的关联分析
Table 3 The association of SNP polymorphisms in MSTN gene with growth traits from three populations of Chinese mitten crab
位点
Locus基因型
Genotype育种群体
Breeding population大赛群体
Contest population野生群体
Wild population总群体
Total populationBW (g) CL (mm) BW (g) CL (mm) BW (g) CL (mm) BW (g) CL (mm) TT 156.50±42.31a 62.45±4.66a 366.35±62.67a 76.16±6.41a 153.75±45.84a 62.57±6.32a 223.78±92.29a 68.41±8.17a SNP1 TC 118.74±50.61bc 56.95±7.53bc 311.79±78.13bc 75.15±4.25ac 160.00±49.15ab 62.28±6.48ab 200.60±
100.54ab65.40±10.92ab CC 88.50±16.30c 52.83±3.48c 302.40±39.32c 66.15±3.15c 113.29±60.02c 55.77±8.54c 124.69±60.95c 56.70±9.16c AA 133.18±50.27ab 59.19±6.82ab 307.06±57.23ab 75.32±4.00ab 155.90±46.35ab 62.85±6.53ab 211.59±96.79ab 66.85±9.28ab SNP2 AG 117.50±48.01bc 56.71±6.88bc 312.38±85.03ac 76.51±6.71ac 147.05±52.53bc 60.50±6.78bc 189.55±96.93bc 64.23±11.09bc GG 106.78±41.00bc 56.11±7.64bc 292.40±46.01bc 75.20±5.10bc 144.02±75.05ac 59.07±10.48ac 183.55±97.79ac 63.66±11.49ac TT 133.98±47.41a 59.47±6.38ab 305.93±56.38ab 75.56±3.96ab 151.03±45.24ab 61.64±6.38ab 205.21±95.11ab 66.29±9.28ab SNP3 TG 118.20±50.86ab 56.59±7.20bc 312.56±80.79ac 76.19±6.50bc 147.33±58.46bc 60.93±7.80bc 198.93±91.47bc 65.03±11.40bc GG 107.58±41.56bc 56.35±6.97bc 300.50±65.66bc 74.57±4.05ac 160.04±50.50ac 62.75±7.06ac 186.43±90.51ac 64.47±9.26ac 注: BW. 体重(g); CL. 壳长(mm); 表中值为平均值±标准误; 同列不同小写字母表示差异显著(P≤0.05, 相同字母差异不显著(P>0.05); 下同 Note: BW. Body weight; CL. Carapace length; values are presented as means ± SE; the different capital letters in the same column indicate significant difference (P≤0.05 and same letters show non-significant difference (P>0.05); the same applies below
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表 4 中华绒螯蟹三群体MSTN基因不同SNP位点组合的生长性状比较
Table 4 The growth traits of different SNP genotype combinations in MSTN gene from the three populations of Chinese mitten crab
群体Populations 性状Traits 基因型Genetype TT1×AA2 TT1×TT3 AA2×TT3 TT1×AA2×TT3 育种群体Breeding population 体重BW (g) 162.97±41.69a 164.50±33.48a 136.93±44.02b 169.55±31.44a 壳长CL (mm) 63.09±4.31a 63.92±2.39a 60.15±5.62b 64.53±2.24a 大赛群体Contest population 体重BW (g) 310.39±64.44a 306.31±64.00a 310.14±58.90a 310.89±67.46a 壳长CL (mm) 75.53±4.54a 75.76±4.60a 75.60±4.26a 75.77±4.87a 野生群体Wild population 体重BW (g) 157.70±44.37a 147.50±50.54a 123.91±43.90b 159.59±45.91a 壳长CL (mm) 63.51±6.05a 63.61±6.92a 60.04±6.08b 63.31±6.39a 总群体Total population 体重BW (g) 229.49±92.20a 228.11±94.08a 209.97±96.70b 233.82±95.24a 壳长CL (mm) 68.86±7.84a 68.06±8.62a 65.71±8.83b 69.48±8.29a
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