COMPARATIVE STUDY OF THE DIFFERENCE IN GLUCOSE AND DEXTRIN UTILIZATION IN THE CHINESE PERCH (SINIPERCA CHUATSI)
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摘要: 研究通过比较鳜(Siniperca chuatsi)对不同碳水化合物的利用差异, 探究肉食性鱼类对碳水化合物利用的分子机制。按照1670 mg/kg剂量对鳜灌喂葡萄糖和糊精后, 分别在0、1h、2h、3h、4h、8h、12h和24h收集水样、血浆、肝脏和肌肉, 检测尿糖、血糖、血甘油三酯、血胰岛素、肝糖原、肌糖原含量及糖代谢相关基因表达水平等指标。结果显示: (1) 灌喂后1—12h内, 两组鳜相比, 葡萄糖组尿糖显著高于糊精组, 血糖及胰岛素含量在两组间无显著差异; (2) 两组鳜甘油三酯含量在2h时达到最大值, 糊精组甘油三酯含量在4h时显著高于葡萄糖组, 糊精组肝糖原含量在1h时显著高于葡萄糖组, 且糊精组肌糖原含量在24h内均显著高于葡萄糖组; (3) 灌喂后1h, 灌喂糊精组葡萄糖激酶(Glucokinase, GK)、脂肪酸合成酶(Fatty Acid Synthetase, FAS)、乙酰辅酶A羧化酶Ⅰ型(Acetyl-CoA Carboxylase Type Ⅰ, ACC1)、柠檬酸合成酶(Citroyl Synthetase, CS)基因表达水平显著高于葡萄糖组, 而在灌喂后8h, 糊精组糖原合酶(Glycogen Synthase, GS)和CS基因表达水平却显著低于葡萄糖组。结果表明, 肉食性鱼类鳜摄入糖后可以促进糖原和脂肪的合成, 转化为糖原和甘油三酯, 从而减少未利用糖的排出, 且鳜对葡萄糖的利用效率低于糊精。Abstract: In this study, we compared the utilization of different carbohydrates in Chinese perch and further explored the molecular mechanism of carbohydrate utilization in carnivorous fish. Water, plasma, liver and muscle samples were collected at 0, 1, 2, 3, 4, 8, 12 and 24h after Chinese perch were fed glucose and dextrin at a 1670 mg/kg dose. The parameters urine sugar, blood glucose, blood triglycerides, blood insulin, and liver and muscle glycogen and the mRNA expression levels of glucose metabolism-related genes were detected. The results showed the following: (1) Within 1—12h after feeding, the blood glucose level was significantly higher in the glucose group than in the dextrin group, while the blood glucose and insulin levels were not significantly different between the two groups. (2) The triglyceride content at 2—4h was higher in the dextrin group than in the glucose group, and the liver glycogen content at 1h was significantly higher in the dextrin group than in the glucose group. Furthermore, the muscle glycogen content at 24h was significantly higher in the dextrin group than in the glucose group. (3) One hour after feeding, the mRNA expression levels of glucokinase (GK), fatty acid synthetase (FAS), acetyl-CoA carboxylase type I (ACC1) and citrate synthase (CS) were significantly higher in the dextran group than in the glucose group, and the expression levels of glycogen synthase (GS) and CS mRNA at 8h were significantly lower in the dextrin group than in the glucose group. These results demonstrated that the utilization efficiency of dextrin was better than that of dextrose and that the intake of dextrin could promote the synthesis of glycogen and fat.
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Keywords:
- Glucose metabolism /
- Siniperca chuatsi /
- Glucose /
- Dextrin /
- Oral administration
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图 1 灌喂不同碳水化合物对鳜尿糖及血糖含量的影响
数据表示为平均值±标准误(n=4), 星号(*)表示同一时间点两组之间显著差异(P<0.05); 不同字母表示两组分别在各个时间点有显著性差异(P<0.05); 下同
Figure 1. Effects of the oral administration of different carbohydrates on the urine glucose and plasma glucose concentrations in Chinese perch
Data are presented as the mean±SEM (n=4). The significance level is marked with an asterisk (P<0.05) and shows significant differences following feeding with different carbohydrates at the same time (P<0.05); different letters indicate significant differences in the same group at various times (P<0.05). The same applies below
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图 2 灌喂不同碳水化合物对鳜血胰岛素、血甘油三酯、肝糖原及肌糖原含量的影响
Figure 2. Effects of the oral administration of different carbohydrates on insulin, triglycerides, hepatic glycogen and muscle glycogen in Chinese perch
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图 3 灌喂不同碳水化合物对鳜GK、FAS、ACC1、PEPCK、GS和CS基因表达水平的影响
Figure 3. The mRNA expression levels of GK, FAS, ACC1, PEPCK, GS and CS in Chinese perch after the oral administration of two different carbohydrates
表 1 本研究实时定量PCR引物
Table 1 Primers used for real-time PCR used in this study
基因Gene 引物序列Sequence of primer (5′—3′) 片段长度Product length (bp) 退火温度Tm (℃) RPL13A CACCCTATGACAAGAGGAAGC 100 59 TGTGCCAGACGCCCAAG GK AAGGTGGAGACCAAGAAC 170 51.5 TGCCCTTGTCAATGTCC FAS ATGGAAATCACCCCTGTAATCTT 203 57 CTTATCTGACTACGGAATGAATCG ACC1 TATGCCCACTTACCCAAATGC 129 58 TGCCACCATACCAATCTCGTT PEPCK CTGAGTTTGTGAAGAGAGCGG 170 57 GTCCTTTGGGTCTGTGCGT GS TACACTGCCTGACCAAGACC 115 54 AATGTGGCTGGAGACGAAT CS GAATGCCACCTACTTCCTTGT 166 57 CCCCTCATACCTCCATAAACC 
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