5种光周期对锦鲤生长、能量收支及生物钟基因表达的影响

史东杰; 王文峰; 李文通; 魏东; 王赛赛; 姜巨峰

doi:10.7541/2021.2021.019

5种光周期对锦鲤生长、能量收支及生物钟基因表达的影响

史东杰^1,2,,
王文峰³,
李文通^1,2,
魏东⁴,
王赛赛^1,2,
姜巨峰^5, ,

1.
北京市农林科学院, 水产科学研究所, 北京 100068
2.
农业农村部华北都市农业重点实验室, 北京 10097
3.
北京市房山区养殖业技术推广站, 北京 102488
4.
天津农学院水产学院, 天津 300380
5.
天津市水产研究所暨天津市观赏鱼技术工程中心, 天津 300221

基金项目: 北京市财政局、北京市农业农村局“观赏鱼产业技术体系北京市创新团队” (BAIC03); 北京市农林科学院“北京淡水鱼种质资源保存”; 天津市财政局、天津市农业农村委“天津市海水养殖产业技术体系海水观赏水族岗位” (ITTMRS2021004); 天津市水产研究所“观赏鱼创新团队” (scyjs201902)资助

详细信息

作者简介:
史东杰(1985—), 女, 副研究员, 硕士; 主要从事鱼类繁育及养殖技术研究工作。E-mail: sdj19850104@163.com

通信作者:
姜巨峰(1981—), 男, 高级工程师, 硕士; 主要从事观赏鱼种质创新及品级提升技术研究。E-mail: jufengjiang@163.com

中图分类号: S965.1
计量
- 文章访问数: 03063
- HTML全文浏览量: 0463
- PDF下载量: 073
出版历程
- 收稿日期: 2021-02-21
- 修回日期: 2021-11-23
- 网络出版日期: 2022-05-08
- 发布日期: 2022-05-14

VARIOUS PHOTOPERIODS ON GROWTH, ENERGY BUDGETS AND GENE EXPRESSION OF CIRCADIAN CLOCK IN KOI CARP (CYPRINUS CARPIO)

1.
Fisheries Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068, China
2.
Key Laboratory of Urban Agriculture (North China), Ministry of Agriculture and Rural Affairs, Beijing 100097, China
3.
Beijing Fangshan District Breeding Industry Technology Promotion Station, Beijing 102488, China
4.
Tianjin Agricultural University Fisheries College, Tianjin 300380, China
5.
Tianjin Fisheries Research Institute, Tianjin Ornamental Fish Technology and Engineering Centre, Tianjin 300221, China

Funds: Supported by the Beijing Municipal Finance Bureau and Beijing Municipal Bureau of Agriculture and Rural Affairs“Beijing Innovation Consortium of Ornamental Fish Research System” (BAIC03); Beijing Academy of Agriculture and Forestry Sciences“Preservation of Beijing Freshwater Fish Germplasm Resources”; Tianjing Municipal Finance Bureau and Tianjing Municipal Bureau of Agriculture and Rural Affairs“The Project for the Ornamental Fish Innovation Team of Tianjin Mariculture Research System (ITTMRS2021004)”; Tianjin Fisheries Research Institute“The Project for the Ornamental Fish Innovation Team” (scyjs201902)

Corresponding author:

摘要

摘要: 研究不同光照周期(长光照18L﹕6D、短光照8L﹕16D、连续光照24L﹕0D、连续黑暗0L﹕24D和对照组12L﹕12D)条件对锦鲤(Cyprinus carpio)生长、能量收支及生物钟基因相对表达量的影响, 实验周期为90d。结果表明, 在整个试验周期, 各光周期处理组成活率无显著变化(P＞0.05), 24L组和18L﹕6D组饲料系数显著低于12L﹕12D组、8L﹕16D组和24D组(P＜0.05)。终末体重、特定生长率在实验前30d内受光照影响不大(P＞0.05), 而实验30d后出现显著变化, 延长光周期尤其是连续光照会显著增加终末体重和特定生长率(P＜0.05); 对比连续黑暗0L﹕24D组, 其他光周期处理组锦鲤摄食能分配在代谢和生长的比例显著增加(P＜0.05); 4个生物钟相关Clock、Per2、Cry1和Bmal1基因相对表达水平无明显规律。综上所述, 延长光照时间, 尤其是连续光照有利于锦鲤幼鱼的生长和发育。
- 光周期 /
- 能量分配 /
- 昼夜节律 /
- 锦鲤
Abstract: This study explored the effects of different photoperiods (long light 18L﹕6D, short light 8L﹕16D, continuous light 24L﹕0D, continuous darkness 0L﹕24D and control group 12L﹕12D) for 90 days on the growth, energy budget and relative expression of circadian clock genes on the juvenile koi (Cyprinus carpio). The results showed that the survival rate of each group had no significant change (P>0.05), and the feed coefficient of 24 L group and 18L﹕6D group was significantly lower than that of 12L﹕12D group, 8L﹕16D group and 24D group (P<0.05). The terminal body weight, feed coefficient and specific growth rate were not affected by light for the first 30 days of the experiment (P>0.05), but significantly changed for the last 30 days of the period. Prolonging light cycle, especially continuous light, significantly increased the terminal body weight and specific growth rate (P<0.05), while the feed coefficient decreased significantly (P<0.05). Compared with the continuous light and dark 240L﹕024D group, the ratio of koi fish feeding energy distribution in metabolism and growth increased significantly in other photoperiod treatment groups (P<0.05). The relative expression levels of Clock, Per2, Cry1 and Bmal1 biological clocks genes have no obvious chaenge. These findings indicate that prolonged illumination time, especially continuous illumination, is beneficial to the growth and development of juvenile fish in koi fish, combined with the experimental results of growth performance, energy budget and biological clock gene expression level under different photoperiods, while prolonged illumination or continuous illumination is beneficial to the growth and development of juvenile fish of koi.
- Photoperiod /
- Energy distribution /
- Day-night rhythm /
- Cyprinus carpio

HTML全文

图 1 不同光照条件下锦鲤增重率的线性回归分析

A-E分别表示不同光照处理条件15d、30d、45d、60d、75d和90d增重率; 所有数据用(χ ±SD, n=30)表示

Figure 1. Linear regressive analysis of koi carp body weight increase rate in different light conditions

A-E represent the weight increase rate in differerent light conditions of 15d, 30d, 45d, 60d, 75d and 90d, respectively. Data are presented as means±SD, n=30

下载: 全尺寸图片幻灯片

图 2 不同光照条件对锦鲤生物钟基因相对表达量的影响

A—D分别表示不同光照处理条件下Clock、Per2、Cry1和Bmal1基因表达量变化; 图中标有不同字母表示同一基因在不同光处理条件下相对表达量差异显著(P＜0.05); 所有数据用(χ±SD, n=10)表示

Figure 2. Effects of different light conditions on the relative expression of four biological clock genes in koi carp

A—D represent the gene expression changes of Clock, Per2, Cry1 and Bmal1 under different light treatment conditions, respectively. Different letters indicate the relative expression of the same gene is significantly different under different light treatment conditions (P＜0.05). Data are presented as means±SD, n=10

下载: 全尺寸图片幻灯片

表 1 本实验所用引物信息

Table 1 Primers used in the study

引物名称 Premer name	序列 Sequence (5′—3′)	产物长度Length of product sequence (bp)
β-actin-F	CTCTATTGTTGTCTATCCT	217
β-actin-R	TCTTTCCCACGAAGCCTTA	217
Clock-F	ATCACCACCTAGGGCCAATA	315
Clock-R	CTACGCCGGTTCTCCTTTCT	315
Cry1-F	GTAGTTCCTGCTATATGGCCT	281
Cry1-R	TACATGCTGCGATTTGTTTTGG	281
Bmal1-F	GTAGACCGGGCGACAGAAG	215
Bmal1-R	CTGAACCAGATGAAACGACTC	215
Per2-F	ATGATCCCGAAACGAGCC	241
Per2-R	CAGTTGCCTTCGATTTTGTGA	241

下载: 导出CSV

表 2 不同光照条件下锦鲤的体重、特定生长率、成活率和饲料系数

Table 2 Body weight, specific growth rate, feed conversion ratio and survival rate of koi carp in different light conditions

时间 Time (d)	光周期L﹕D Different light conditions	初始体重 Initial body weight (g)	终末体重 Terminal body weight (g)	特定生长率SGR Specific growth rate (%/d)	饲料系数FCR Feed conversion ratio	成活率SR Survival rate (%)
15	24:0	127.52±5.62	164.47±8.04	1.70±0.05	2.13±0.15^b	98.66±3.76
	18:6	126.28±5.11	163.13±7.95	1.71±0.06	2.24±0.12^b	100.00±0.00
	12:12	127.32±5.34	163.04±7.91	1.65±0.05	2.44±0.18^a	99.33±3.52
	8:16	125.23±5.13	161.29±8.06	1.69±0.05	2.46±0.18^a	100.00±0.00
	0:24	125.39±4.69	161.11±8.11	1.67±0.06	2.43±0.17^a	98.00±2.1.9
30	24:0	125.38±4.91	208.23±11.63	1.69±0.05	2.02±0.11^c	100.00±0.00
	18:6	125.3±5.13	206.87±11.74	1.67±0.06	2.11±0.09^c	99.33±4.07
	12:12	124.89±5.14	205.66±12.16	1.66±0.05	2.45±0.15^b	100.00±0.00
	8:16	127.32±5.34	208.92±11.64	1.65±0.04	2.54±0.16^a	99.33±2.94
	0:24	127.29±4.98	207.09±12.09	1.62±0.04	2.51±0.16^a	99.32±3.12
45	24:0	126.38±5.36	280.19±13.16^a	1.71±0.07^a	2.01±0.12^d	100.00±0.00
	18:6	127.93±5.63	272.22±13.47^a	1.74±0.07^a	2.09±0.10^d	99.32±4.32
	12:12	127.87±5.09	273.28±13.21^a	1.69±0.06^a	2.34±0.17^c	99.32±3.78
	8:16	125.08±5.54	241.97±10.52^b	1.47±0.05^b	2.52±0.21^b	99.32±3.40
	0:24	124.62±5.61	237.08±10.37^b	1.43±0.05^b	3.15±0.36^a	100.00±0.00
60	24:0	128.93±4.68	361.81±18.91^a	1.72±0.05^a	2.03±0.09^c	100.00±0.00
	18:6	126.83±4.87	353.33±19.13^a	1.71±0.07^a	2.00±0.10^c	100.00±0.00
	12:12	125.91±5.13	348.55±16.41^b	1.70±0.08^a	2.73±0.12^b	100.00±0.00
	8:16	126.73±5.46	312.19±15.88^c	1.50±0.04^b	2.68±0.14^b	100.00±0.00
	0:24	125.02±5.15	307.07±16.02^c	1.50±0.05^b	3.42±0.22^a	100.00±0.00
75	24:0	127.78±5.32	458.22±21.34^a	1.70±0.05^a	2.01±0.12^c	100.00±0.00
	18:6	128.17±5.26	447.19±22.16^a	1.67±0.06^a	2.08±0.10^c	99.32±3.21
	12:12	128.38±5.19	438.24±20.38^b	1.64±0.03^a	2.89±0.16^b	99.32±2.93
	8:16	126.17±5.37	378.11±19.13^c	1.46±0.05^b	2.95±0.18^b	98.65±3.95
	0:24	125.09±5.51	368.87±19.34^c	1.44±0.02^b	3.28±0.23^a	97.95±3.84
90	24:0	128.93±4.91	587.98±25.46^a	1.69±0.06^a	2.19±0.12^d	100.00±0.00
	18:6	127.88±4.96	573.12±25.69^a	1.67±0.06^a	2.21±0.13^d	100.00±0.00
	12:12	128.93±5.40	554.65±21.18^b	1.62±0.05^a	2.99±0.18^c	100.00±0.00
	8:16	128.02±5.11	483.33±21.47^c	1.48±0.07^b	3.02±0.32^b	100.00±0.00
	0:24	126.94±5.31	429.61±19.64^c	1.35±0.05^c	3.36±0.35^a	100.00±0.00
注: 表中标有不同字母表示同一时间段不同光处理条件间差异显著(P＜0.05); 所有数据用(χ±SD, n=30)表示; 下同Note: Different letters on top of the column denote significant differences between treatments at given time points (P＜0.05). Data are presented as means±SD, n=30. The same applies below

下载: 导出CSV

表 3 不同光照条件下锦鲤的能量总收支

Table 3 Total energy budget of koi under different light conditions (%)

L﹕D	C	P/C	R/C	F/C	U/C
24﹕0	100	31.16±4.01^a	56.46±4.63^a	4.11±0.83^b	8.27±0.74^b
18﹕6	100	30.81±3.64^a	55.12±4.78^a	5.68±0.81^b	8.39±0.93^b
12﹕12	100	31.45±3.58^a	54.27±4.32^a	5.47±0.75^b	8.81±0.82^b
8﹕16	100	29.73±3.87^a	55.45±5.11^a	5.83±0.76^b	8.99±0.94^b
0﹕24	100	24.27±3.67^b	46.27±4.79^b	10.44±1.21^a	19.02±0.67^a
注: C. 摄食能; P/C. 总生长能/摄食能; R/C. 代谢能/摄食能; F/C. 粪便能/摄食能; U/C. 排泄能/摄食能Note: C. Feeding energy; P/C. Total growth energy/Feeding energy; R/C. Metabolic energy/Feeding energy; F/C. Fecal energy/Feeding energy; U/C. Excretory energy/Feeding energy

下载: 导出CSV

参考文献(29)

[1]	Matthew K L, Vahid Z, Ian A E. Growth and survival of winter flounder (Pseudopleuronectes americanus) larvae reared on different photoperiod regimes from hatch to metamorphosis [J]. Aquaculture Research, 2020, 51(6): 2314-2321. doi: 10.1111/are.14575
[2]	Edurne B, Patricia R, Aurelio O, et al. The effects of light, darkness and intermittent feeding on the growth and survival of reared Atlantic bonito and Atlantic bluefin tuna larvae [J]. Aquaculture, 2017(479): 233-239.
[3]	Kim B H, Hur S P, Hur S W, et al. Relevance of light spectra to growth of the rearing tiger puffer Takifugu rubripes [J]. Development & Reproduction, 2016, 20(1): 23-29.
[4]	Helvik J V, Walther B T. Photo-regulation of the hatching process of halibut (Hippoglossus hippoglossus) eggs [J]. Journal of Experimental Zoology, 1992, 263(2): 204-209. doi: 10.1002/jez.1402630210
[5]	Huber M, Bengtson D A. Effects of photoperiod and temperature on the regulation of the onset of maturation in the estuarine fish Menidia beryllina (Cope) (Atherinidae) [J]. Journal of Experimental Marine Biology & Ecology, 1999, 240(2): 285-302.
[6]	魏平平, 李鑫, 刘鹰, 等. 光周期对红鳍东方鲀脑组织中GH和SS基因表达水平和昼夜表达模式的影响 [J]. 大连海洋大学学报, 2020, 35(1): 108-113. Wei P P, Li X, Liu Y, et al. Effects of photoperiod on expression level and daily expression pattern of GH and SS genes in brain of tiger puffer Takifugu rubripes [J]. Journal of Dalian Ocean University, 2020, 35(1): 108-113.
[7]	刘春晓, 吕为群, 杨志刚, 等. TGF-β/Smad信号通路响应光周期变化参与调控斑马鱼卵巢发育 [J]. 南方水产科学, 2019, 15(3): 68-75. doi: 10.12131/20180286 Liu C X, Lu W Q, Yang Z G, et al. TGF-β/Smad signaling pathway responding to photoperiod for participation in regulation of zebrafish ovarian development [J]. South China Fisheries Science, 2019, 15(3): 68-75. doi: 10.12131/20180286
[8]	袁满, 王鹏飞, 闫路路, 等. 花鲈垂体和下丘脑中生物钟基因在3种光周期下的表达节律分析 [J]. 南方水产科学, 2020, 16(6): 39-46. Yuan M, Wang P F, Yan L L, et al. Circadian rhythmicity of clock genes in pituitary and hypothalamus of spotted sea perch (Lateolabrax maculates) under three photoperiod conditions [J]. South China Fisheries Science, 2020, 16(6): 39-46.
[9]	Brown E E, Baumann H, Conover D O. Temperature and photoperiod effects on sex determination in a fish [J]. Journal of Experimental Marine Biology & Ecology, 2014, 461: 39-43.
[10]	Shin H S, Song J A, Choi J Y, et al. Effects of various photoperiods on Kisspeptin and reproductive hormones in the goldfish [J]. Animal Cells & Systems, 2014, 18(2): 109-118.
[11]	陈孝红, 仇雪梅, 郝薇薇, 等. 斑马鱼CYP11a1基因在不同性腺发育时期的表达 [J]. 大连海洋大学学报, 2015, 30(1): 13-17. doi: 10.3969/J.ISSN.2095-1388.2015.01.003 Chen X H, Qiu X M, Hao W W, et al. Expression of CYP11a1 in different developmental phases of gonad in zebrafish Danio rerio [J]. Journal of Dalian Ocean University, 2015, 30(1): 13-17. doi: 10.3969/J.ISSN.2095-1388.2015.01.003
[12]	Teng X L, Zhang Z, He G L, et al. Validation of reference genes for quantitative expression analysis by real-time RT - PCR in four lepidopteran insects [J]. Journal of Insect Science, 2012, 12(60): 1-17. doi: 10.1673/031.012.6001
[13]	张俊功, 戴习林, 丁福江. 水温和雌雄配比对罗氏沼虾能量代谢的影响 [J]. 南方农业学报, 2020, 51(7): 1721-1728. doi: 10.3969/j.issn.2095-1191.2020.07.027 Zhang J G, Dai X L, Ding F J. Effects of temperature and female-male mating ratios on energy metabolism of Macrobrachium rosenbergii [J]. Journal of Southern Agriculture, 2020, 51(7): 1721-1728. doi: 10.3969/j.issn.2095-1191.2020.07.027
[14]	刘晓娟, 郭勋, 王春芳, 等. 基于生物能量学原理构建异育银鲫生长、饲料需求和污染排放模型 [J]. 水生生物学报, 2018, 42(2): 221-231. doi: 10.7541/2018.028 Liu X J, Guo X, Wang C F, et al. Bioenergetics-based model to determine growth, feed requirement and waste output of gibel carp (Carassius auratus gibelio) [J]. Acta Hydrobiologica Sinica, 2018, 42(2): 221-231. doi: 10.7541/2018.028
[15]	Abdollahpour H, Falahatkar B, Lawrence C. The effect of photoperiod on growth and spawning performance of zebrafish, Danio rerio [J]. Aquaculture Reports, 2020, 17: 100295. doi: 10.1016/j.aqrep.2020.100295
[16]	Wei H, Cai W J, Liu H K, et al. Effects of photoperiod on growth, lipid metabolism and oxidative stress of juvenile gibel carp (Carassius auratus) [J]. Journal of Photochemistry & Photobiology B: Biology, 2019, 198: 111552.
[17]	Striberny A, Lauritzen D E, Fuentes J, et al. More than one way to smoltify a salmon? Effects of dietary and light treatment on smolt development and seawater growth performance in Atlantic salmon [J]. Aquaculture, 2021, 532(15): 736044.
[18]	Lundova K, Matousek J, Stejskal V. The effect of non-circadian photoperiod on growth and puberty onset of brook trout Salvelinus fontinalis mitchill [J]. Animals, 2021, 11(3): 692. doi: 10.3390/ani11030692
[19]	Wilkinson R J, Longland R, Woolcott H, et al. Effect of elevated winter-spring water temperature on sexual maturation in photoperiod manipulated stocks of rainbow trout (Oncorhynchus mykiss) [J]. Aquaculture, 2010, 309(1-4): 236-244. doi: 10.1016/j.aquaculture.2010.08.023
[20]	Falahatkar B, Poursaeid S, Efatpanah I, et al. Growth, development and behaviour of Persian sturgeon Acipenser persicus larvae in different light regimes [J]. Aquaculture Research, 2017, 48(12): 5812-5820. doi: 10.1111/are.13404
[21]	Edgar A. Aragón F, Leonardo M C, Crisantema H G, et al. Effect of light intensity and photoperiod on growth and survival of the Mexican cichlid, Cichlasoma beani in culture conditions [J]. Latin American Journal of Aquatic Research, 2017, 45(2): 293-301. doi: 10.3856/vol45-issue2-fulltext-5
[22]	Windarti W, Amin B, Simarmata A H. Growth and health status of Pangasionodon hypophthalmus reared under manipulated photoperiod conditions [J]. F1000 Research, 2021, 26(10): 154.
[23]	Vondracek B, Wurtsbaugh W A, Cech J J. Growth and reproduction of the mosquitofish Gambusia affinis, in relation to temperature and ration level: consequences for life history [J]. Environmental Biology of Fishes, 1988(21): 45-57. doi: 10.1007/BF02984442
[24]	黄建盛, 王安利, 陈刚, 等. 低盐水体下摄食水平对斜带石斑鱼幼鱼生长及能量收支的影响 [J]. 海洋科学, 2016, 40(11): 113-120. Huang J S, Wang A L, Chen G, et al. Effect of feeding level on the growth and energy budgets of Epinephelus coioides juveniles cultured in low-salt water [J]. Marine Sciences, 2016, 40(11): 113-120.
[25]	林小涛, 杞桑, 曹双俊, 等. 光周期对罗氏沼虾幼体生长及能量收支的影响 [J]. 海洋与湖沼, 1998, 29(2): 119-127. doi: 10.3321/j.issn:0029-814X.1998.02.003 Lin X T, Qi S, Cao S J, et al. Influence of photoperiods on growth and energy budget of Macrobrachium rosenbergii larvae [J]. Oceanologia et Limnologia Sinica, 1998, 29(2): 119-127. doi: 10.3321/j.issn:0029-814X.1998.02.003
[26]	Choi J Y, Choi Y U, Kho J, et al. Effects of various photoperiods and specific wavelengths on circadian rhythm in ornamental cleaner shrimp Lysmata amboinensis [J]. Biological Rhythm Research, 2019, 50(6): 897-907. doi: 10.1080/09291016.2018.1502237
[27]	Albrecht U. Timing to perfection: the biology of central and peripheral circadian clocks [J]. Neuron, 2012, 74(2): 246-260. doi: 10.1016/j.neuron.2012.04.006
[28]	Tamai T K, Young L C, Whitmore D. Light signaling to the zebrafish circadian clock by Cryptochrome 1a [J]. Proceedings of the National Academy of Sciences of the United States of America, 2007, 104(37): 14712-14717. doi: 10.1073/pnas.0704588104
[29]	Gavriouchkina D, Fischer S, Ivacevic T, et al. Thyrotroph embryonic factor regulates light-induced transcription of repair genes in zebrafish embryonic cells [J]. PLoS One, 2010, 5(9): e12542. doi: 10.1371/journal.pone.0012542

施引文献

资源附件(0)

图(2) / 表(3)

计量

文章访问数:
HTML全文浏览量:
PDF下载量:
被引次数: 0

5种光周期对锦鲤生长、能量收支及生物钟基因表达的影响

作者简介: 史东杰(1985—), 女, 副研究员, 硕士; 主要从事鱼类繁育及养殖技术研究工作。E-mail: sdj19850104@163.com

通信作者: 姜巨峰(1981—), 男, 高级工程师, 硕士; 主要从事观赏鱼种质创新及品级提升技术研究。E-mail: jufengjiang@163.com

计量

出版历程

VARIOUS PHOTOPERIODS ON GROWTH, ENERGY BUDGETS AND GENE EXPRESSION OF CIRCADIAN CLOCK IN KOI CARP (CYPRINUS CARPIO)

Corresponding author:

计量

出版历程

目录

作者简介:
史东杰(1985—), 女, 副研究员, 硕士; 主要从事鱼类繁育及养殖技术研究工作。E-mail: sdj19850104@163.com

通信作者:
姜巨峰(1981—), 男, 高级工程师, 硕士; 主要从事观赏鱼种质创新及品级提升技术研究。E-mail: jufengjiang@163.com