Abstract: To investigate the ecotoxicologial effects of dietary phenanthrene (PHE) to the juvenile Spinibarbus sinensis (42.800.32 g), these fish were exposed to dietary PHE at different concentrations (0, 500, 1000 and 1500 g PHE/g) for 16 weeks. The haematological indexes and the hepatopancreas index were utilized to measure the ecotoxicologial effects of dietary PHE. The results showed that the addition of PHE at different concentrations had no significant effects in the number of red blood cells, hemoglobin and total protein in the serum. PHE increased the glucose level with a concentration dependent pattern. 500 g PHE/g significantly enhanced the globulin and the albumin level compared with control group; however, the higher PHE at 1000 and 1500 g PHE/g had no significant effects compared with the control. PHE induced the activity of alanine aminotransferase with a concentration-dependent method, and the significant difference was observed when PHE is at 1500 g PHE/g (P0.05). PHE also enhanced the activity of aspartate aminotransferase, and the significant effects were observed at the groups of both 1000 and 1500 g PHE/g. PHE at all different levels significantly decreased the content of glycogen in hepatopancreas(P0.05). PHE decreased the muscle glycogen in the three groups but not significant. PHE dramatically and significantly enhanced the hepatopancreas (P0.05). These results suggest that increasing glucose content and decreasing of glycogen and protein content might be an adaptive response to meet the energy demand for resisting the PHE stress, which might compensated by the increasing hepatopancreas index, and that PHE might damage the liver cells supported by the increasing activities of the two aminotransferases.