白屈菜红碱对铜绿微囊藻生长和光合系统的影响
Effect of chelerythrine on the growth and photosynthetic system of Microcystis aeruginosa
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摘要: 为了阐明白屈菜红碱(Chelerythrine)对铜绿微囊藻(Microcystis aeruginosa)生长及光合系统的影响, 研究了白屈菜红碱胁迫下铜绿微囊藻M. aeruginosa NIES-843生长、细胞色素含量及叶绿素光诱导荧光动力学变化. 结果显示, 当白屈菜红碱浓度10 g/L时, M. aeruginosa NIES-843的生长受到显著抑制. 通过线性回归分析, 白屈菜红碱对M. aeruginosa NIES-843生长50%抑制浓度(EC50)为(30.621.32) g/L. 当白屈菜红碱浓度为160 g/L时, M. aeruginosa NIES-843单位细胞内叶绿素a (Chl. a)和类胡萝卜素含量均显著低于对照. Chl. a光诱导荧光动力学分析结果显示, 白屈菜红碱胁迫下单位反应中心吸收的光能(ABS/RC)、单位反应中心捕获的用于还原QA的能量(TR0/RC)及单位反应中心捕获的用于电子传递的能量(ET0/RC)均受到显著抑制. 光合系统Ⅱ(PSⅡ)能量分配比率参数分析结果显示, 白屈菜红碱能显著抑制光合系统反应中心电子供体侧电子传递.Abstract: The effects of chelerythrine on the growth, pigments contents and photosynthetic system of Microcystis aeruginosa NIES-843 were studied in this paper. The results indicated that the growth of M. aeruginosa NIES-843 was inhibited by chelerythrine at the concentration of 10 g/L. Regression analysis showed that the EC50 of chelerythrine on the growth of M. aeruginosa NIES-843 was (30.621.32) g/L. Intracellular pigments of M. aeruginosa NIES-843 decreased at the concentration of 160 g/L. Results of chlorophyll fluorescence transient analysis suggested that the absorbed energy (ABS/RC), the trapped energy for reduce of QA (TR0/RC) and the trapped energy for electron transport (ET0/RC) were all inhibited by chelerythrine. Energy fluxes ratios analysis of PSⅡ indicated that the electrons providing side of reaction centre was the targets of toxicity of chelerythrine on M. aeruginosa NIES-843.
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