陈政, 刘翠, 刘昊昆, 朱晓鸣, 韩冬, 杨云霞, 金俊琰, 解绶启. 亚硒酸钠、酵母硒和富硒螺旋藻对杂交鲟幼鱼生长、抗氧化能力及组织硒含量的影响[J]. 水生生物学报, 2024, 48(1): 44-52. DOI: 10.7541/2023.2023.0040
引用本文: 陈政, 刘翠, 刘昊昆, 朱晓鸣, 韩冬, 杨云霞, 金俊琰, 解绶启. 亚硒酸钠、酵母硒和富硒螺旋藻对杂交鲟幼鱼生长、抗氧化能力及组织硒含量的影响[J]. 水生生物学报, 2024, 48(1): 44-52. DOI: 10.7541/2023.2023.0040
CHEN Zheng, LIU Cui, LIU Hao-Kun, ZHU Xiao-Ming, HAN Dong, YANG Yun-Xia, JIN Jun-Yan, XIE Shou-Qi. DIETARY SODIUM SELENITE, SELENIUM YEAST AND SELENIUM-ENRICHED SPIRULINA ON GROWTH, ANTIOXIDANT CAPACITY AND TISSUE SELENIUM CONTENTS OF JUVENILE HYBRID STURGEON (ACIPENSER BAERII ♂×ACIPENSER SCHRENCKII ♀)[J]. ACTA HYDROBIOLOGICA SINICA, 2024, 48(1): 44-52. DOI: 10.7541/2023.2023.0040
Citation: CHEN Zheng, LIU Cui, LIU Hao-Kun, ZHU Xiao-Ming, HAN Dong, YANG Yun-Xia, JIN Jun-Yan, XIE Shou-Qi. DIETARY SODIUM SELENITE, SELENIUM YEAST AND SELENIUM-ENRICHED SPIRULINA ON GROWTH, ANTIOXIDANT CAPACITY AND TISSUE SELENIUM CONTENTS OF JUVENILE HYBRID STURGEON (ACIPENSER BAERII ♂×ACIPENSER SCHRENCKII ♀)[J]. ACTA HYDROBIOLOGICA SINICA, 2024, 48(1): 44-52. DOI: 10.7541/2023.2023.0040

亚硒酸钠、酵母硒和富硒螺旋藻对杂交鲟幼鱼生长、抗氧化能力及组织硒含量的影响

DIETARY SODIUM SELENITE, SELENIUM YEAST AND SELENIUM-ENRICHED SPIRULINA ON GROWTH, ANTIOXIDANT CAPACITY AND TISSUE SELENIUM CONTENTS OF JUVENILE HYBRID STURGEON (ACIPENSER BAERII ♂×ACIPENSER SCHRENCKII ♀)

  • 摘要: 实验旨在研究饲料中不同类型和水平的硒源对杂交鲟( Acipenser baerii ♂× Acipenser schrenckii ♀)幼鱼生长、抗氧化能力及组织硒含量的影响。不同类型的硒源为亚硒酸钠、酵母硒和富硒螺旋藻, 添加水平为0、0.4和1.2 mg/kg, 制作对照饲料(C)、亚硒酸钠添加饲料(S1和S2)、酵母硒添加饲料(Y1和Y2)和富硒螺旋藻添加饲料(P1和P2)。使用实验饲料饲喂初始体重为(7.82±0.12) g的杂交鲟幼鱼, 养殖62d。结果表明, 不同硒源和硒水平对杂交鲟幼鱼的特定生长率和饲料效率无显著影响(P>0.05)。高水平亚硒酸钠显著提高了全鱼和肝脏硒含量(P<0.05), 但对肌肉和脊椎骨硒含量无显著影响(P>0.05)。高水平酵母硒和富硒藻螺旋添加组杂交鲟幼鱼的全鱼、肝脏、肌肉及脊椎骨硒含量均显著高于对照组(P<0.05)。酵母硒添加组的血浆总蛋白(TP)和总胆固醇(TC)含量均显著高于对照组(P<0.05)。同时, 高水平硒添加组杂交鲟幼鱼的血浆谷胱甘肽过氧化物酶(GSH-Px)活性显著高于对照组和低水平硒添加组(P<0.05)。亚硒酸钠组肌肉硒含量与全鱼硒含量显著正相关(P<0.05), 与肝脏硒含量显著负相关(P<0.05)。血浆GSH-Px活性与肌肉、脊椎骨和肝脏硒含量正相关(P<0.05)。综上所述, 高水平硒添加可以提高全鱼及肝脏硒含量, 不同类型硒在鱼体的蓄积模式不一致, 高水平的有机硒(酵母硒和富硒螺旋藻)添加比无机硒(亚硒酸钠)更易在肌肉和脊椎骨中蓄积; 高水平硒添加可显著提高杂交鲟抗氧化能力, 不同硒源对杂交鲟的抗氧化能力无显著影响。

     

    Abstract: This experiment aimed to investigate the effects of different sources and levels of dietary selenium additives on the growth, antioxidant capacity and tissue selenium contents of hybrid sturgeon juveniles. Three sources of selenium were selected as sodium selenite, selenium yeast and selenium-enriched spirulina at two different levels of 0, 0.4 and 1.2 mg/kg, to prepare control diet (C) and sodium selenite supplemented diets (S1, S2), selenium yeast supplemented diets (Y1, Y2) and selenium-enriched spirulina supplemented diets (P1, P2). Hybrid sturgeon juveniles (initial body weight at about 7.8 g) were raised for 62d using the experimental diets. The results showed that different selenium sources and levels had no significant effects on the specific growth rate and feed efficiency of juvenile hybrid sturgeon (P>0.05), however the selenium contents in the whole fish, liver, muscle and vertebra of the hybrid sturgeon in the high yeast selenium and high selenium-enriched spirulina supplemented groups were significantly higher than those in the control group (P<0.05). Sodium selenite supplementation significantly increased the selenium contents in the whole fish and liver (P<0.05), but had no significant effects on the selenium contents in muscle and vertebra (P>0.05). Muscle selenium content in sodium selenite group was significantly positively correlated with whole fish selenium content (P<0.05), and significantly negatively correlated with liver selenium content (P<0.05). Meanwhile, the plasma GSH-Px activity of the juvenile hybrid sturgeon in the high selenium supplemented groups was significantly higher than that in the control and low selenium supplemented groups (P<0.05), furthermore, the MDA contents in the selenium-enriched spirulina supplemented group were significantly lower than those in the sodium selenite supplemented groups (P<0.05). Plasma GSH-Px activity was positively correlated with muscle (P<0.01), vertebra (P<0.01) and hepatic selenium content (P<0.05). The plasma total protein (TG) and total cholesterol (TC) contents in the selenium yeast supplemented groups were significantly higher than those in the control group (P<0.05). In conclusion, high-level selenium supplementation can increase the selenium contents of whole fish and liver, but the accumulation patterns of different types of selenium in fish are inconsistent. High-level organic selenium (selenium yeast and selenium-enriched spirulina) supplementation is more likely to accumulate in muscle and vertebrae than inorganic selenium (sodium selenite). Moreover, high-level selenium supplementation could significantly improve the antioxidant capacity of hybrid sturgeon, and different selenium sources had no significant effects on antioxidant properties.

     

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