雌性斑马鱼暴露于环境剂量三唑磷对亲代生殖和氧化应激及子代胚胎发育的影响

FEMALE ZEBRAFISH EXPOSED TO ENVIRONMENTAL DOSES OF TRIAZOPHOS ON PARENTA REPRODUCTION, OXIDATIVE STRESS AND EMBRYONIC DEVELOPMENT OF OFFSPRING

  • 摘要: 三唑磷作为一种广泛使用的有机磷杀虫剂, 该药物在自然水体中的残留可对非靶标生物产生潜在威胁, 文章旨在探究三唑磷对斑马鱼的毒性效应。研究将3月龄雌性斑马鱼(Danio rerio) 持续暴露于环境相关浓度的三唑磷(10 μg/L) 21d, 然后与健康雄鱼自然受精产卵; 通过测定雌鱼生殖力、亲代与子代的氧化应激及子代胚胎发育相关指标, 评估三唑磷对亲代雌鱼及其子代的毒性效应。主要研究结果: 亲代雌鱼经10 μg/L三唑磷暴露后, 产卵量显著提高(P<0.05), 肝脏内乙酰胆碱酯酶(AChE)活性显著降低(P<0.05), 丙二醛(MDA)含量显著增加(P<0.05), 超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽-S-转移酶(GST)和羧酸酯酶(CarE)活性均显著增强(P<0.05); 所产子代胚胎受精率、受精卵直径、胚胎心率和初孵仔鱼体长均显著降低(P<0.05), 仔鱼畸形率显著增加(P<0.05), 孵化率无显著性差异; 仔鱼AChE与SOD活性呈下降趋势但差异不显著, MDA含量显著增加(P<0.05), GPx与GST活性显著降低(P<0.05), CarE活性显著提高(P<0.05)。以上研究结果表明环境浓度三唑磷21d暴露可提高雌性斑马鱼的生殖力, 诱导亲代及子代氧化应激反应, 并对子代胚胎发育过程产生不良影响。

     

    Abstract: Triazophos is a widely used organophosphorus insecticide. The residual amount of triazophos detected in natural water may pose a potential threat to non-target aquatic organisms. This paper aims to investigate the toxic effect of triazophos on zebrafish. In this study, 3-month-old female zebrafish (Danio rerio) were continuously exposed to environmentally relevant concentrations of triazophos (10 µg/L) for 21d. Subsequently, natural spawning and fertilization were performed using healthy males, and the toxicity of triazophos on both the parental females and their offspring was assessed. The assessment included measurements of female fertility, oxidative stress in parent and offspring, as well as indicators of embryonic development in the offspring. The main results were as follows: after exposure to 10 µg/L triazophosphos, the spawning capacity of parental female fish increased significantly (P<0.05), moreover, it caused a significantly decrease in the activity of liver acetylcholinesterase (AChE) (P<0.05), an increase in malondialdehyde (MDA) content (P<0.05), and a significant enhancement in the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST) and carboxylesterase (CarE) (P<0.05). The embryo fertilization rate, fertilized egg diameter, embryo heart rate and body length of newly hatched larvae were significantly lower (P<0.05), while the malformation rate of larval fish was significantly higher (P<0.05). However, there was no significant difference in the hatching rate (P>0.05). The activity of AChE and SOD in larvae decreased although not significant (P>0.05), whereas the MDA content increased significantly (P<0.05). Furthermore, the activities of GPx and GST were significantly decreased (P<0.05), while CarE activity was significantly increased (P<0.05). The above findings suggest that exposure to environmental concentrations of triazophos can increase female zebrafish's fecundity, cause oxidative stress in the parenta and their offspring, and adversely affect the developmental process of the offspring embryos.

     

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