一种检测微囊藻毒素LR诱导大鼠肾细胞凋亡的方法
DETECTION OF APOPTOSIS OF RENAL CELLS OF MICE INDUCED BY MICROCYSTIN-LR
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摘要: 微囊藻毒素(Microcystins)是一种具有生物活性的单环七肽毒素,主要由微囊藻产生。其中5个氨基酸为固定组成成分,另外2种氨基酸是可变的,由此衍生出众多的同类物,微囊藻毒素LR(Microcystin LR,MCLR)是其中具有代表性的一种。同位素示踪显示,静脉注射、腹腔注射和口服3种不同途径进入小鼠体内的125I-MCLR 70%以上分布在肝脏和肾脏,揭示这两个脏器是它的靶器官。本实验中采用正常大鼠的肾细胞来研究MCLR对细胞的影响,利用流式细胞仪PI/Annexin V双染色法检测MCLR能否引起细胞凋亡的改变,为进一步探讨MCLR对细胞的损伤及作用机制提供依据。
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关键词:
- 微囊藻毒素 /
- NRK细胞 /
- PI(propidium iodide) /
- Annexin V /
- 细胞凋亡
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Keywords:
- Microcystin LR /
- NRK cells /
- PI;Annexin V /
- Apoptosis
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[1] Sui H X, Yan W X, Xu H B. Toxicity of microcystin and its bioaccumulation effect [J].J Hygiene Res. 2002,31(3):214-217.[隋海霞,严卫星,徐海滨. 微囊藻毒素的毒性以及水生生物的富集作用. 卫生研究,2002,31(3):214-217][2] Zhang Z Y, Yu S Z, Wei G R,et al. Study on the whole and cells level distribution of microcystin LR in mice [J]. J Health Toxicol. 2002,16(1):5-8.[张占英,俞顺章,卫国荣,等.微囊藻毒素LR在动物体内整体水平及细胞水平的分布.卫生毒理学杂志,2002,16(1):5-8][3] Vermes I.Hannen C, Steffens-Nakken H et al. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled Annexin V [J].J Immunol Meth.1995,184:39-51[4] Schindl A, Klosner G. Flow cytometric quantification of UV-induced cell death in a human aquamous cell carcinoma-derived cell line:dose and kinetic studies [J].J Photochem Photobiol B,1998,44:97-106 Sui H X, Yan W X, Xu H B. Toxicity of microcystin and its bioaccumulation effect [J].J Hygiene Res. 2002,31(3):214-217.[隋海霞,严卫星,徐海滨. 微囊藻毒素的毒性以及水生生物的富集作用. 卫生研究,2002,31(3):214-217][2] Zhang Z Y, Yu S Z, Wei G R,et al. Study on the whole and cells level distribution of microcystin LR in mice [J]. J Health Toxicol. 2002,16(1):5-8.[张占英,俞顺章,卫国荣,等.微囊藻毒素LR在动物体内整体水平及细胞水平的分布.卫生毒理学杂志,2002,16(1):5-8][3] Vermes I.Hannen C, Steffens-Nakken H et al. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labeled Annexin V [J].J Immunol Meth.1995,184:39-51[4] Schindl A, Klosner G. Flow cytometric quantification of UV-induced cell death in a human aquamous cell carcinoma-derived cell line:dose and kinetic studies [J].J Photochem Photobiol B,1998,44:97-106
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