IGF-ImRNA在不同年龄鲤表达的差异和LHRH-A对IGF-ImRNA表达的影响
DIFFERENTIAL EXPRESSION AND EFFECT OF LHRH-A ON EXPRESSION OF IGF-I mRNA IN JUVENILE AND ADULT COMMON CARP(CYPRINUS CARPIO)
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摘要: 利用RNA酶保护法对7月龄性未成熟幼鲤和2龄性成熟鲤组织胰岛素样生长因子-I(IGF-I)mRNA的表达水平进行测定,结果表明成鱼肝和肾脏组织IGF-ImRNA的丰度显着高于幼鱼.对鲤成鱼和幼鱼腹腔注射促性腺激素释放激素类似物(LHRH-A,D-Ala6-Pro9-NEt-LHRH)使血清生长激素(GH)水平和肝组织IGF-ImRNA水平都显着升高,而成鱼生殖腺IGF-ImRNA的丰度比对照组显着增加.研究结果提示鲤在不同发育阶段肝组织IGF-ImRNA的表达存在差别,其中2龄成鱼大于7月龄幼鱼;LHRH-A可能通过刺激垂体GH的释放间接促进肝组织IGF-ImRNA的表达,亦可能通过某种未知途径刺激生殖腺IGF-ImRNA的表达.
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关键词:
- 鲤 /
- 胰岛素样生长因子-I /
- 年龄 /
- LHRH-A
Abstract: IGF-I mRNA expression of 7-month old juvenile and 2-year old adult common carp were determined by RNase Protection Assay. Results showed that the hepatic IGF-I mRNA level of adult fish was significantly higher than that of 7-month old juvenile fish; while in other tissues there was no significant difference between the two fish groups except that kidney IGF-I mRNA level of adult fish was higher than that of juvenile fish. After twice i. p. injections of LHRH-A, the levels of serum GH and hepatic IGF-I mRNA in fish of both ages increased greatly, and the IGF-I mRNA level of gonadal tissue in adult fish increased when compared with controls. No changes in amount of IGF-I mRNA in other tissues were observed after injections of LHRH-A. It is suggested that the level of hepatic IGF-I mRNA expression changes with different developmental stages; LHRH-A may stimulate the production of IGF-I by promoting the synthesis and release of pituitary GH; and probably, LHRH-A stimulates the IGF-I expression in gonads by other mechanism.-
Keywords:
- Common carp /
- Insulin-like growth factor-I /
- Age /
- LHRH-A
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[1] Duanc. Tissue-specific expression of insulin-like growth factor I messenger ribonucleic acids in salmonids: developmental, hormonal, and nutritional regulation [A]. In:"Perspectives in Comparative Endocrinology”. 365-372. National Research Council of Canada[2] Shamblott M J, Chen T T. Age related and tissue-specific levels of five form of insulin-like growth factor mRNA in a teleost [J]. Molecular Marine Biology and Biotechnology, 1993,2(6):351-361[3] 王黎,林浩然,张为民. 阿扑吗啡对LHRH-A促进鲤鱼GtH和GH分泌的影响[J]. 中山大学学报(自然科学版),1997,36:119-121[4] 华益民,林浩然,钟翎. 用RNase保护法定量检测鲤鱼组织IGF-I mRNA的表达[J]. 中山大学学报(自然科学版),1998,37(4):121-124[5] 林浩然. 鱼类生长和生长激素分泌活动的调节[J]. 动物学报,1996,42(1):69-79 Duanc. Tissue-specific expression of insulin-like growth factor I messenger ribonucleic acids in salmonids: developmental, hormonal, and nutritional regulation [A]. In:"Perspectives in Comparative Endocrinology”. 365-372. National Research Council of Canada[2] Shamblott M J, Chen T T. Age related and tissue-specific levels of five form of insulin-like growth factor mRNA in a teleost [J]. Molecular Marine Biology and Biotechnology, 1993,2(6):351-361[3] 王黎,林浩然,张为民. 阿扑吗啡对LHRH-A促进鲤鱼GtH和GH分泌的影响[J]. 中山大学学报(自然科学版),1997,36:119-121[4] 华益民,林浩然,钟翎. 用RNase保护法定量检测鲤鱼组织IGF-I mRNA的表达[J]. 中山大学学报(自然科学版),1998,37(4):121-124[5] 林浩然. 鱼类生长和生长激素分泌活动的调节[J]. 动物学报,1996,42(1):69-79
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