草鱼体内肾细胞姐妹染色单体分化及交换的初步研究

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PRELIMINARY STUDIES ON FREQUENCIES OF SISTER CHROMATID DIFFERENTIAL(SCD) AND EXCHANGES(SCE) IN RENAL CELLS OF GRASS CARP(CTENOPHARYGODON IDELLUS) IN VIVO

摘要: 本文确立了一个以草鱼体内肾细胞姐妹染色单体交换频率为指标的检测环境诱变或致癌物质的短期试验系统。采用硫堇-UV-Giemsa染色法,分析了草鱼体内肾细胞的SCD-2(注射BrdU后第二个细胞周期的中期分裂相的SCD)频率和SCE频率。用500微克/克体重BrdU体内标记5天,草鱼肾细胞SCD-2频率为8.58±0.22%;SCE频率为3.05±2.523 SCE5/细胞。以丝裂霉素C(Mitomycin C,MMC)作为阳性对照,分析了化合物亚硝基胍(N-methyl-N1-nitro-N-nitrosoguanidine,MNNG)和农药叶蝉散(Mipc)诱发SCE的能力。这项工作在评价水质污染方面将起一定的作用。

Abstract: Using the frequency of sister chromatid exchanges (SCE) as an indicator, we have developed an in vivo system for assessing environmental mutagenic agents and water pollutants which act upon fish. The sister chromatid differential (SCD) and SCE were demonstrated by thionine-UV-Giemsa procedures 5 days after the fish having been injected with 500 μg/g of BrdU (5-bromodeoxyuridine). The frequencies of SCD and SCE in renal cells of grass carp were found to be 8.58±0.22% and 3.05±2.523 per metaphase, respectively. By using Mitomycin C (MMC) as a positive control, the abilities of MNNG and Mipc to induce SCE were analyzed. This system is considered to be a useful tool for water pollution monitoring.