DIAGNOSIS OF IRIDOVIRUS DISEASE IN SCIAENOP SOCELLATUS
-
摘要: 对广东省珠海市每年6—10月池塘养殖的眼斑拟石首鱼(Sciaenop socellatus)暴发不明原因疾病进行了研究。通过对该病的流行病学调查，结合症状观察、病原分离鉴定、病理学变化，结果显示：该病具有明显的传染性，解剖病鱼可见鳃丝贫血、脾脏和肾脏肿大、出血，通过镜检未检测到大量的寄生虫；从发病鱼的肝、脾、肾脏和肠中未分离到致病菌；在病鱼肝、脾和肾组织病理切片中观察到有大量肿大细胞；通过使用OIE 推荐的虹彩病毒特异性引物进行PCR扩增出570 bp左右的目的片段，MCP基因序列比对显示扩增获得的基因序列与真鲷虹彩病毒(RSIV)的基因序列同源性高达99.2%。综合以上结果表明导致眼斑拟石首鱼发病死亡的原因是感染了细胞肿大属虹彩病毒而引起的虹彩病毒病。Abstract: The epidemiological investigation, symptom observation, pathogenic detection, and the pathology detection were used to examine the cause of the disease of Sciaenop socellatus of cultured pond in Zhuhai, Guangdong Province from June to October. These results showed that the disease had obvious infectious. The anatomy examination revealed the anemia of the gill filament, swollen spleen and kidney with hemorrhagic spots, and no observed large number of parasites by microscopy. Meanwhile, no pathogenic bacteria were isolated from the liver, spleen, kidney and intestine of the diseased fish. A large number of swelling cells were observed in pathological sections of liver, spleen and kidney of diseased fish. The 570 bp fragment was amplified by PCR using the specific primers of OIE. MCP sequence alignment showed that the amplification of the gene sequence had 99.2% of homology with gene sequence of RSIV. The comprehensive results showed that the cause of the disease of the Sciaenop socellatus is infected by the iridovirus of Megalocytivirus genus.
-
Keywords:
- Sciaenop socellatus /
- Iridovirus disease /
- Megalocytivirus /
- Diagnosis
-
-
![]()
图 1 病鱼解剖特征
A．眼间皮肤褪; B. 尾鳍出血; C. 鳃丝贫血; D. 肝脏贫血发白; E. 脾脏肿大; F. 肾脏肿大
Figure 1. Characteristics of diseased fish
A. Fade eye skin; B. Bleeding of the caudal fin; C. Anemia of the gill; D. Anemia of the liver; E. Enlargement of the spleen; F. Enlargement of the kidney
![]()
图 2 眼斑拟石首鱼组织病变情况
A、C、E、G. 健康眼斑拟石首鱼组织; B、D、F、H. 患病眼斑拟石首鱼组织; A、B. 肝; C、D. 脾; E、F. 肾; G、H. 肠; 箭头示肿大细胞; lc. 肝细胞; rc. 红血细胞; bd. 胆管; fb. 脂肪空泡; lp. 淋巴细胞; mc. 单核细胞; nc. 坏死细胞; rt. 肾小管; g. 肾小球; ce. 柱状上皮细胞; HE染色400×
Figure 2. The pathological changes of the tissue of Sciaenops ocellatus
A. C. E. G. The health tissues of Sciaenops ocellatus; B. D. F. H. the diseased tissue of Sciaenops ocellatus; A. B. liver; C. D. spleen; E. F. kidney; G. H. intestines; Black arrows indicate mast cells; Lc. hepatocyte; rc. hepatocyte; bd. bile duct; fb. fat vacuole; lp. leukomonocyte; mc. monocyte nc: dead cells; rt. kidney tubules; g: glomeruli of kidney; ce. columnar epithelial cell; HE staining 400×
-
[1] Zhang Y Q, Gui J F. Interacting the host with the viral genomes and viruses of the aquatic animals [J]. Scientia Sinica Vitae, 2014, 44(12): 1236—1252 [张奇亚, 桂建芳. 水产动物的病毒基因组及其病毒与宿主的相互作用. 中国科学: 生命科学, 2014, 44(12): 1236—1252] Zhang Y Q, Gui J F. Interacting the host with the viral genomes and viruses of the aquatic animals [J]. Scientia Sinica Vitae, 2014, 44(12): 1236—1252 [张奇亚, 桂建芳. 水产动物的病毒基因组及其病毒与宿主的相互作用. 中国科学: 生命科学, 2014, 44(12): 1236—1252]
[2] Kusuda R, Nagato K, Kawai K. Characteristics of an Iridovirus isolated from Red Sea Bream, Pagrus major [J]. Aquaculture Science, 2010, 42(1): 151—156
[3] Oh S Y, Kim W S, Oh M J, et al. Multiplication Rate of Red Seabream Iridovirus (RSIV) in Rock Bream Oplegnathus fasciatus at different fish rearing temperatures [J]. Fish Pathology, 2017, 51(4): 194—198
[4] Dong Y, Weng S, He J, et al. Field trial tests of FKC vaccines against RSIV genotype Megalocytivirus in cage-cultured mandarin fish ( Siniperca chuatsi) in an inland reservoir [J]. Fish & Shellfish Immunology, 2013, 35(5): 1598—1603
[5] Razak A A, Ransangan J, Sade A. First report of Megalocytivirus (Iridoviridae) in grouper culture in Sabah, Malaysia [J]. International Journal of Current Microbiology and Applied Sciences, 2014, 3(3): 896—909
[6] Chen M H, Hung S W, Chang C H, et al. Red sea bream iridovirus infection in marble goby (Bleeker, Oxyeleotris marmoratus) in Taiwan [J]. Academic Journals, 2013, 7(12): 1009—1014
[7] Wang Q, Zeng W W, Liu C, et al. A duplex PCR for detection of iridescent virus from largemouth bass, Micropterus salmoides [J]. Journal of Huazhong Agricultural University, 2013, 32(4): 106—110 [王庆, 曾伟伟, 刘春, 等. 大口黑鲈虹彩病毒双重PCR检测方法的建立. 华中农业大学学报, 2013, 32(4): 106—110] Wang Q, Zeng W W, Liu C, et al. A duplex PCR for detection of iridescent virus from largemouth bass, Micropterus salmoides [J]. Journal of Huazhong Agricultural University, 2013, 32(4): 106—110 [王庆, 曾伟伟, 刘春, 等. 大口黑鲈虹彩病毒双重PCR检测方法的建立. 华中农业大学学报, 2013, 32(4): 106—110]
[8] Li X F, Tian L X, Niu J, et al. Effects of dietary lipid level on growth performance, body composition and liver histological structure of red drum ( Sciaenop socellatus) [J]. Chinese Journal of Animal Nutrition, 2015, 27(11): 3448—3456 [李雪菲, 田丽霞, 牛津, 等. 饲料脂肪水平对美国红鱼生长性能、体组成和肝脏组织结构的影响. 动物营养学报, 2015, 27(11): 3448—3456] Li X F, Tian L X, Niu J, et al. Effects of dietary lipid level on growth performance, body composition and liver histological structure of red drum ( Sciaenop socellatus) [J]. Chinese Journal of Animal Nutrition, 2015, 27(11): 3448—3456 [李雪菲, 田丽霞, 牛津, 等. 饲料脂肪水平对美国红鱼生长性能、体组成和肝脏组织结构的影响. 动物营养学报, 2015, 27(11): 3448—3456]
[9] Zhou Q C, J. Alejandro Buentello, Delbert M. Gatlin Ⅲ. Effects of dietary prebiotics on growth performance, immune response and intestinal morphology of red drum ( Sciaenops ocellatus) [J]. Aquaculture, 2010, 309(1): 253—257
[10] Wen L, Lei Y, Qi R R, et al. Diagnosis of iridovirus disease in sea bass Lates calcarifer [J]. Chinese Journal of Fisheries, 2015, 28(4): 28—32 [文琳, 雷燕, 戚瑞荣, 等. 尖吻鲈Lates calcarifer虹彩病毒病的诊断. 水产学杂志, 2015, 28(4): 28—32] Wen L, Lei Y, Qi R R, et al. Diagnosis of iridovirus disease in sea bass Lates calcarifer [J]. Chinese Journal of Fisheries, 2015, 28(4): 28—32 [文琳, 雷燕, 戚瑞荣, 等. 尖吻鲈Lates calcarifer虹彩病毒病的诊断. 水产学杂志, 2015, 28(4): 28—32]
[11] Wang C S, Shih H H, Ku C C, et al. Studies on epizootic iridovirus infection among red sea bream, Pagrus major, (Temminck & Schlegel), cultured in Taiwan [J]. Journal of Fish Diseases, 2003, 26(3): 127—133
[12] Wang Q, Zeng W W, Liu C, et al. Identification and chara cterization analysis on amegalocytivirus isolated from diseased marbled sleepy goby, Oxyeleotris marmoratus [J]. Acta Hydrobiologica Sinica, 2010, 34(6): 1150—1156 [王庆, 曾伟伟, 刘春, 等. 云斑尖塘鳢肿大细胞病毒属虹彩病毒的分离与鉴定. 水生生物学报, 2010, 34(6): 1150—1156] Wang Q, Zeng W W, Liu C, et al. Identification and chara cterization analysis on amegalocytivirus isolated from diseased marbled sleepy goby, Oxyeleotris marmoratus [J]. Acta Hydrobiologica Sinica, 2010, 34(6): 1150—1156 [王庆, 曾伟伟, 刘春, 等. 云斑尖塘鳢肿大细胞病毒属虹彩病毒的分离与鉴定. 水生生物学报, 2010, 34(6): 1150—1156]
[13] Zhang J T, Zhu W Y, Wang G L. Pathogeny and Pathogenicity of Kidney Intumesce of Sciaenops ocellatus [J]. Journal of Ningbo University( NSEE ) , 2013, 26(1): 6—11 [张继挺, 朱文渊, 王国良. 美国红鱼肾肿大症的病原及其致病性研究. 宁波大学学报(理工版), 2013, 26(1): 6—11] Zhang J T, Zhu W Y, Wang G L. Pathogeny and Pathogenicity of Kidney Intumesce of Sciaenops ocellatus [J]. Journal of Ningbo University(
NSEE ) , 2013, 26(1): 6—11 [张继挺, 朱文渊, 王国良. 美国红鱼肾肿大症的病原及其致病性研究. 宁波大学学报(理工版), 2013, 26(1): 6—11] [14] Lin Z S, Zhang P, Wang T G, et al. Drug screening and application of Sciaenops ocellatus streptococcosis in Dong-tou [J]. Journal of Aquaculture, 2015, 36(1): 23—26 [林振士, 张鹏, 王铁杆, 等. 洞头县美国红鱼链球菌病药物筛选及应用. 水产养殖, 2015, 36(1): 23—26] Lin Z S, Zhang P, Wang T G, et al. Drug screening and application of Sciaenops ocellatus streptococcosis in Dong-tou [J]. Journal of Aquaculture, 2015, 36(1): 23—26 [林振士, 张鹏, 王铁杆, 等. 洞头县美国红鱼链球菌病药物筛选及应用. 水产养殖, 2015, 36(1): 23—26]
[15] Yao H, Huang Y C, Cai S H, et al. Isolation and Identifica tion of Bacterial Pathogens from Sciaenops ocellatus with Tail-rotted Disease and Evaluation of Immune Effi cacy of Inactived Vaccine [J]. Journal of Guangdong Ocean University, 2016, 36(4): 37—44 [姚欢, 黄郁葱, 蔡双虎, 等. 眼斑拟石首鱼烂尾病病原菌的分离鉴定及灭活疫苗的免疫效果. 广东海洋大学学报, 2016, 36(4): 37—44] Yao H, Huang Y C, Cai S H, et al. Isolation and Identifica tion of Bacterial Pathogens from Sciaenops ocellatus with Tail-rotted Disease and Evaluation of Immune Effi cacy of Inactived Vaccine [J]. Journal of Guangdong Ocean University, 2016, 36(4): 37—44 [姚欢, 黄郁葱, 蔡双虎, 等. 眼斑拟石首鱼烂尾病病原菌的分离鉴定及灭活疫苗的免疫效果. 广东海洋大学学报, 2016, 36(4): 37—44]
[16] Ma A M, Yan M C, Hu L H, et al. Identification of a Vibrio splendidus strain isolated from ill Sciaenops ocella tus [J]. Acta Microbiologica Sinica, 2010, 50(4): 542—547 [马爱敏, 闫茂仓, 胡利华, 等. 患溃烂症的眼斑拟石首鱼分离的灿烂弧菌的鉴定. 微生物学报, 2010, 50(4): 542—547] Ma A M, Yan M C, Hu L H, et al. Identification of a Vibrio splendidus strain isolated from ill Sciaenops ocella tus [J]. Acta Microbiologica Sinica, 2010, 50(4): 542—547 [马爱敏, 闫茂仓, 胡利华, 等. 患溃烂症的眼斑拟石首鱼分离的灿烂弧菌的鉴定. 微生物学报, 2010, 50(4): 542—547]
[17] Wang Y Y, Guo G W, Gao R C, et al. Turbellarian Parasiitsedin Sciaenops ocellatus and its control [J]. Journal of Fisheries of China, 2002, 26(4): 379—381 [汪彦愔, 郭果为, 高如承, 等. 一种危害眼斑拟石首鱼的寄生涡虫及其防治. 水产学报, 2002, 26(4): 379—381] Wang Y Y, Guo G W, Gao R C, et al. Turbellarian Parasiitsedin Sciaenops ocellatus and its control [J]. Journal of Fisheries of China, 2002, 26(4): 379—381 [汪彦愔, 郭果为, 高如承, 等. 一种危害眼斑拟石首鱼的寄生涡虫及其防治. 水产学报, 2002, 26(4): 379—381]
[18] Li K B, Shi C B, Li X H, et al. Pathogen of infective outbreaking disease in Sciaenop ocellatus [J]. Journal of Fishery Sciences of China, 2002, 9(1): 56—59 [李凯彬, 石存斌, 李新辉, 等. 眼斑拟石首鱼暴发性传染病病原初步研究. 中国水产科学, 2002, 9(1): 56—59] Li K B, Shi C B, Li X H, et al. Pathogen of infective outbreaking disease in Sciaenop ocellatus [J]. Journal of Fishery Sciences of China, 2002, 9(1): 56—59 [李凯彬, 石存斌, 李新辉, 等. 眼斑拟石首鱼暴发性传染病病原初步研究. 中国水产科学, 2002, 9(1): 56—59]
下载:
计量
- 文章访问数: 2088
- HTML全文浏览量: 701
- PDF下载量: 44