PROMOTIONAL EFFECTS OF TWO MACROALGAL EXTRACTS ON THE GROWTH AND METABOLIC ACTIVITY OF DUNALIELLA SALINA
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摘要:
实验选取绿潮藻提取物和铜藻提取物作为一种高经济价值藻——杜氏盐藻生长的营养添加剂, 与传统的f/2培养基相比, 探究两种大型海藻提取物对杜氏盐藻的生长和某些代谢活性(色素、碳水化合物、蛋白质、光合活性)的影响。藻类生理指标和营养成分分析表明, 不同浓度的藻华提取物对杜氏盐藻生长的促进效果显著。添加0.26%、0.31%、0.38 %的绿潮藻提取物和添加0.24%的铜藻提取物均能够显著促进杜氏盐藻生物量增长、光合色素和营养物质的积累, 但对杜氏盐藻的最大光化学效率(Fv/Fm)和有效量子产量(Yield)无显著影响。研究表明, 一定浓度的绿潮藻提取物和铜藻提取物能够显著促进杜氏盐藻的生长和营养物质的积累, 该发现为藻华防控及利用大型藻华提取物来优化杜氏盐藻的生产提供了新的思路。
Abstract:In this experiment, Green Tide Algae Extract and Copper Algae Extract were selected as nutrient additives for cultivating Dunaliella salina, a high-value alga, aiming to explore the effects on the growth and various metabolic activities (such as pigments, carbohydrates, proteins, and photosynthetic activities) compared to the traditional f/2 medium. The physiological indices and nutrient analysis of the algae showed that different concentrations of macroalgal extracts significantly promoted the growth of Dunaliella salina. Specifically, the addition of 0.26%, 0.31%, and 0.38% of Chlorella vulgaris extract, as well as 0.24% of Copper Algae extract, notably promoted biomass growth, photosynthetic pigment content, and nutrient accumulation in Dunaliella salina. However, these additions did not significantly impact the maximum photochemical efficiency (Fv/Fm) and effective quantum yield (Yield) of Duchenne Saltwater Algae. The present study demonstrated that specific concentrations of Chlorophyceae and Copperophyceae extracts could significantly promote the growth and nutrient accumulation of Duchenne salina. This findings provide a novel approach for algal bloom prevention and control, along with optimizing the production of Dunaliella salina through the utilization of macroalgal bloom extracts.
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图 2 杜氏盐藻细胞密度与吸光度A684的关系
Figure 2. Relationship between cell density and A684 of Dunaliella salina
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图 3 施加绿潮藻粉水提物15d内杜氏盐藻的OD值在684 nm下的变化情况
标准误差柱顶部的符号表示对照组和实验组数据之间差异的显著性。“*”代表差异显著(P<0.05), “**”代表差异极显著(P<0.01); 下同
Figure 3. Changes in OD of Dunaliella salina at 684 nm during 15d of aqueous extract application of green tide algae
Symbols on the top of standard error bars indicate the significant differences between data for control and experimental group. “*” represent significant difference (P<0.05), “**” represent higly significant difference (P<0.01). The same applies below
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图 4 施加铜藻粉水提物15d内杜氏盐藻的OD值在684 nm下的变化情况
Figure 4. Changes in OD of Dunaliella salina at 684 nm during 15d of aqueous extract application of Sargassum horneri
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图 5 施加绿潮藻粉水提物15d内盐藻藻液中的叶绿素a、叶绿素b和β-胡萝卜素含量的变化情况
Figure 5. Changes in chlorophyll a, chlorophyll b and β-carotene contents of Dunaliella salina algal solution within 15d of aqueous extracts application of green tide algae
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图 6 施加铜藻粉水提物15d内盐藻藻液中的叶绿素a、叶绿素b和β-胡萝卜素含量的变化情况
Figure 6. Changes in chlorophyll a, chlorophyll b, and β-carotene content of Dunaliella salina algal solution during 15d of aqueous extracts application of Sargassum horneri
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图 7 添加绿潮藻粉水提物后15d内杜氏盐藻最大光化学效率(Fv/Fm)和有效量子产量(Yeild)的变化情况
Figure 7. Changes in Maximum photochemical efficiency (Fv/Fm), and effective quantum yield (Yeild), of Dunaliella salina during 15d after aqueous extract addition of green tide algae
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图 8 施加铜藻粉水提物后15d内杜氏盐藻最大光化学效率(Fv/Fm)和有效量子产量(Yeild)的变化情况
Figure 8. Changes in Maximum photochemical efficiency (Fv/Fm), and effective quantum yield (Yeild) of Dunaliella salina during 15d after aqueous extract addition of Sargassum horneri
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图 9 施加绿潮藻粉水提物后15d内盐藻藻液中总糖含量和可溶性蛋白质含量的变化
Figure 9. Changes in total sugar content and soluble protein content of Dunaliella salina algal solution during 15d after aqueous extracts application of green tide algae
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