不同壳色三角帆蚌外套膜基因的SRAP-cDNA差异分析
ANALYSIS OF DIFFERENTIAL GENE EXPRESSION BY SRAP-cDNA IN MANTLE TISSUE OF HYRIOPSIS CUMINGII WITH DIFFERENT EXTERNAL SHELL COLOR
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摘要: 三角帆蚌幼蚌壳表面颜色和放射条纹表型遗传分离均符合孟德尔单基因位点的一对等位基因调控规律。研究为进一步阐释其壳色遗传分子调控机制， 应用群体分离分析法（BAS）和SRAP-cDNA 比较了两种典型外壳色表型三角帆蚌外套膜组织的基因表达差异。采用30个引物组合获得5个差异片段， 回收克隆测序获得14条差异序列， 大小在195-339 bp之间， 通过序列比对， 获得与2个相似蛋白序列， 包括类二氢嘧啶脱氢酶和类锌指MYM-1型蛋白， 而未发现与色素合成相关基因和蛋白， 但三角帆蚌外壳色表型差异是否与嘧啶代谢和类锌指蛋白参与的基因表达调控有关还有待进一步研究。Abstract: The segregation of shell coloration and presence of radial rays in the juvenile freshwater mussel, Hyriopsis cumingii, follows Mendelian inheritance modes of a single gene locus with one pair of dominant/recessive alleles. To elucidate the mechanisms of external shell color formation, gene expression of two typical shell colors was analyzed by bulked segregant analysis (BSA) and SRAP-cDNA. Five differential gene segments were obtained by SRAP-cDNA using 30 primers' combinations, and 14 fragments with the size ranging from 195 bp to 339 bp were obtained after rec-laim and clone. Using BlastX, these fragments could encode two similar proteins, dihydropyrimidine dehydrogenase (DPD) and MYM-1 Zn Finger protein, and no protein related to pigment could be encoded. Further directions should focus on the relationship between shell color phenotype and pyrimidine metabolism by similar Zn finger proteins in the triangle pearl mussels.
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Keywords:
- Hyriopsis cumingii /
- Shell color phenotype /
- Mantle tissue /
- SRAP-cDNA /
- Differential genes
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