The morphological and truss network characters of three populations of Tinca tinca, e.g., two indigenous populations from Zhujiahu Lake and 73 Reservoir of Xinjiang, China, and one exotic population from Czech, were studied by three multivari-ate analysis (cluster analysis, discriminant analysis and principal component analysis). The results showed that Zhujiahu popula-tion has a close relationship with 73 Reservoir population, and Czech population is far from other two populations.This was in ac-cord with the geographical distribution of the three populations. With 20 primers screened out from 60 primers, using RAPD(random amplified polymorphic DNA)technique, these three populations were analyzed.The proportions of polymophic loci of Zhujiahu Lake, 73 Reservoir and Czech were 24 %, 22. 67 % and 18. 42 %respectively.The genetic diversity index (л)was 0. 1539,0. 1489 and 0. 1142 respectively.These indicated that Zhujiahu population had higher genetic diversity than the others.The genetic similarity among three populations was 0. 6868-0. 9496.The fixation index (Fst)was 0. 048-0. 238. Variation within population accounted for 83. 96 %of total variation and variation between populations was only 16. 04 %of total variation.It was concluded, accordingly, that the genetic differention among three populations was low.