Abstract: This study developed a method to detect sarafloxacin in goldfish (Carassius auratus) plasma and tissues using reverse phase high performance liquid chromatography (RP2HPLC).Plasma,muscle,skin,hepatopancreas,kidney and ovary were sampled at different intervals after oral administration of sarafloxacin hydrochloride at single dose of 20mg/kg under 21±2 ℃.Joining danofloxacin mesylate as inner standard , the medicine was extracted by dichloromethane from plasma and tissues,and the extracts were evaporated to dryness with N2 flow in water bath of 60℃then dissolved in mobile phase . The fat of the solute was degreased by hexane.Sarafloxacin hydrochloride and danofloxacin mesylate analyses were performed by RP2HPLC.The mobile phase was acetonitrile and 0.12mol/L phosphate buffer (pH714) (including 015 % tetrabutyl ammonium bromide)(10/90,V/ V) adjusted to pH 310 with phosphoric acid.Sarafloxacin hydrochloride and danofloxacin mesylate were quantitated by using ZORAX SB2C18 column and ultraviolet detector was set at 280nm.The mean recoveries were all higher than 82.97% for samples and inter-day CV for plasma and tissues were all under 8.41%,The lowest limit of detection was 010125μg/g for muscle.Both limit of detection (LOD) and limit of quantitation (LOQ) met the requirement of the residual detection.The results showed that the elimination rate was markedly different in plasma and five tissues of healthy female goldfish. The drug was slowly eliminated in plasma,the concentration of sarafloxacin hydrochloride in kidney and hepatopancreas was higher than other tissues and skin showed the slowest depletion among five tissues.Kidney was the main reservoir of sarafloxacin hy- drochloride in goldfish.Withdrawl time was 14d when the maximum residue is 30μg/kg for edible tissues after single oral administration of sarafloxacin hydrochloride in healthy female goldfish.