鳜鱼病毒病原的检出及组织病理分析

DETECTION OF VIRAL PATHOGEN FROM DISEASED MANDARIN FISH (SINIPERCA CHUATSI) WITH PATHOLOGICAL OBSERVATIONS

  • 摘要: 经对患暴发性传染病的鳜组织及病变细胞超薄切片的电镜观察,在细胞质、核及间隙中都有病毒颗粒。对该病毒进行了分离提纯,这是一种直径约为280um球形病毒。将粗提病毒液接种到培养细胞中,会引起细胞病变;病毒通过人工感染健康鳜,会引起鳜发病和死亡。对患病鳜进行解剖和组织病理观察,表明病鳜的5种器官都发生了病变。研究结果表明所分离的鳜病毒(Siniperca chuatsi virus,SCV)是引起鳜流行病的病原。

     

    Abstract: Electron microscopy of ultrathin sections were conducted in liver, spleen and heart of the mandarin fish (Siniperca chuatsi Basilewsky) with outbreak of acute lethal disease. Spherical virus particles of 280um in diameter were found distributed in cytoplasm, nuclei and gaps of the cells. The particles were isolated from tissues of the infected fish. When the filtered tissue extracts from diseased fish were inoculated onto culturing confluent cells, cytopathogeic effects were induced in the infected cells.Health fish was experimetally infected by the tissue extract from diseased fish or amplificated virus extracts from culture cells, and lethal disease of the fish was resulted. Anatomical and histopatholgical studies were performed in gill, liver, spleen, heart and kidney from naturally and antificially diseased fishes infected by the virus, and were compared and analysed with same organs of health individuals. It is supported that the virus found from the fish siniperca chuatsi is the lethal pathogen of the infective disease of the fish Siniperca chuatsi.

     

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