饲料中添加抗菌肽Surfactin对吉富罗非鱼肠道健康的影响
EFFECT OF DIETARY ANTIMICROBIAL PEPTIDES-SURFACTIN SUPPLEMENTATION ON PARAMETERS OF INTESTINAL HEALTH INDICES OF GENETICALLY IMPROVED FARMED TILAPIA (GIFT, OREOCHROMIS NILOTICUS)
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摘要: 为研究饲料中添加抗菌肽Surfactin对吉富罗非鱼(GIFT, Oreochromis niloticus)肠道健康指标的影响, 实验将平均体重为(12.010.03) g/尾的320尾吉富罗非鱼, 随机分为4组, 每组4个重复, 每个重复20尾鱼, 分别投喂抗菌肽Surfactin添加水平为0(对照组)、50、100和200 mg/kg的试验饲料7周。结果显示: 与对照组相比, 吉富罗非鱼饲料中添加抗菌肽Surfactin可使肠道皱襞高度显著增加(P0.05), 肌层厚度无显著变化(P0.05); 大肠杆菌数量显著降低(P0.05), 乳酸杆菌数量显著增加(P0.05), 细菌总数无显著变化(P0.05); 显著提高肠道总抗氧化能力水平、谷胱甘肽过氧化物酶活性和超氧化物歧化酶活性(200 mg/kg抗菌肽添加组除外)(P0.05), 显著降低肠道丙二醛水平(P0.05), 对过氧化氢酶活性无显著影响(P0.05); 吉富罗非鱼肠道健康指标以50 mg/kg抗菌肽Surfactin添加组最佳。试验表明, 吉富罗非鱼饲料中适量添加抗菌肽Surfactin可增加肠道皱襞高度、调节肠道菌群和提高肠道抗氧化能力而改善肠道健康状态。Abstract: A 7-week feeding trial was conducted to evaluate effects of antimicrobial peptides-surfactin on intestinal health indices of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Three hundred and twenty fish with initial average weight of (12.010.12) g were randomly divided into four treatment groups with four replicates and 20 fish in each replicate. The fish were fed experimental diets supplemented with 0 (control group), 50, 100, and 200 mg/kg antimicrobial peptides-surfactin, respectively. Surfactin supplementation increased intestinal folds height of GIFT (P0.05) but did not impact the muscular thickness (P0.05). Surfactin supplementation significantly decreased the number of Escherichia coli of GIFT (P0.05) and significantly increased the number of Lactobacillus (P0.05). There was no significant difference in the total bacterial number among all treatment groups (P0.05). Surfactin supplementation significantly increased the total antioxidant capacity level and activities of glutathion peroxidase and superoxide dismutase (except 200 mg/kg Surfactin supplementation group) (P0.05) but significantly decreased the content of malondialdehyde (P0.05). Surfactin supplementation had no effect on catalase activity (P0.05). The intestinal health parameters of 50 mg/kg antimicrobial peptides-surfactin supplementation group were the best among all supplementation groups. In conclusion, surfactin supplementation at 50 mg/kg could improve intestinal health status of GIFT by increasing the intestinal folds height and regulating intestinal microbial population and antioxidant ability.
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