MOLECULAR CLONING OF LVANT2 GENE AND ITS EXPRESSION PATTERN BY COLD INDUCTION

YIN Qin; CUI Liang; PENG Jin-Xia; WEI Pin-Yuan; XIE Da-Xiang; CHEN Xiu-Li; WANG Zhi-Wei; LI Kui; CHEN Xiao-Han

doi:10.3724/SP.J.1035.2012.00024

Volume 36 Issue 1

Mar. 2014

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ACTA HYDROBIOLOGICA SINICA > 2012 > 36(1): 24-28. > DOI: 10.3724/SP.J.1035.2012.00024

YIN Qin, CUI Liang, PENG Jin-Xia, WEI Pin-Yuan, XIE Da-Xiang, CHEN Xiu-Li, WANG Zhi-Wei, LI Kui, CHEN Xiao-Han. MOLECULAR CLONING OF LVANT2 GENE AND ITS EXPRESSION PATTERN BY COLD INDUCTION[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(1): 24-28. DOI: 10.3724/SP.J.1035.2012.00024

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MOLECULAR CLONING OF LVANT2 GENE AND ITS EXPRESSION PATTERN BY COLD INDUCTION

Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Institute of Fisheries, Nanning 530021, China;
2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100094, China

Received Date: July 15, 2010
Rev Recd Date: May 23, 2011
Published Date: January 24, 2012

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Abstract

To study the molecular mechanism of cold tolerance of Litopenaeus`vannamei, a 360 bp EST was isolated from suppression subtractive hybridization library of hepatopancreas which treated by low temperature. The 360 bp EST shared highly homology with ANT2 gene of other species, so we named it Litopenaeus vannamei ANT2 gene (LVANT2). Then we cloned whole cDNA sequence of PVANT2 from a hepatopancreas cDNA library. The LVANT2 gene was 1540 bp with a 1011 bp open reading frame encoding a peptide of 309aa. Tissue expression pattern by real-time PCR revealed that LVANT2 mRNA existed in heart, hepatopancreas, muscle, stomach, intestinal and eyestalk but mainly existed in muscle tissue. Cold induction by different temperatures revealed that LVANT2 mRNA expression started to increase after 15℃ treatment for 36 hours, and then decreased after 13℃ treatment for 36 hours, but increased again after 11℃ treatment. As for 13℃ treatment for different times, the expression pattern changed during the first 12 hours, but the expression with low temperature treatment for 24 hours and 36 hours was almost the same with the room temperature. The low temperature induction expression pattern demonstrated that LVANT2 may function in cold tolerance of Litopenaeus vannamei.
- LVANT2,
- Expression,
- SSH library,
- Full-length cDNA library

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