Volume 38 Issue 2
Mar.  2014
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LIU Jun, LIU Zhi-Zhi, WANG Cheng-Hui, XIANG Song-Ping, WANG Jian. THE ANALYSIS OF THE POLYMORPHISM AND EXPRESSION OF MHC CLASS Ⅱ GENE AND ITS ASSOCIATION WITH RESISTANCE/SUSCEPTIBILITY TO AEROMONAS HYDROPHILA IN XINGGUO RED COMMON CARP[J]. ACTA HYDROBIOLOGICA SINICA, 2014, 38(2): 241-248. DOI: 10.7541/2014.36
Citation: LIU Jun, LIU Zhi-Zhi, WANG Cheng-Hui, XIANG Song-Ping, WANG Jian. THE ANALYSIS OF THE POLYMORPHISM AND EXPRESSION OF MHC CLASS Ⅱ GENE AND ITS ASSOCIATION WITH RESISTANCE/SUSCEPTIBILITY TO AEROMONAS HYDROPHILA IN XINGGUO RED COMMON CARP[J]. ACTA HYDROBIOLOGICA SINICA, 2014, 38(2): 241-248. DOI: 10.7541/2014.36
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THE ANALYSIS OF THE POLYMORPHISM AND EXPRESSION OF MHC CLASS Ⅱ GENE AND ITS ASSOCIATION WITH RESISTANCE/SUSCEPTIBILITY TO AEROMONAS HYDROPHILA IN XINGGUO RED COMMON CARP

  • Oujiang Color Common Carp Provincial Farm, Longquan 323700, China

  • Received Date: August 08, 2013
  • Rev Recd Date: November 01, 2013
  • Published Date: March 24, 2014
    Graphical Abstract
    • Abstract
  • We cloned 171 sequences of MHC class Ⅱ gene were determined from 41 susceptible and 22 resistant individuals of Cyprinus carpio var. singguoensis. These sequences belonged to 26 alleles, thirteen of which were novel (Cyca-DXA24-Cyca-DXA36). The total length of the sequence was 624 bp, consisting of a 33 bp exon1, a 252 bp exon2, a 285 bp exon3, and a 54 bp exon4. These exons encoded a partial signal peptide, a 1 domain, a 2 domain and a connecting peptide (CP)/partial transmembrane (TM), respectively. The mutation-Prone positions of nucleotide or amino acid sites in 1 domain (55.16%, 79.76%) significantly out number than those in 2 domain (45.96%, 68.42%). The values (= dN/dS) were 5.742 for peptide-binding region (PBR), 0.755 for non-PBR in the 1 domain and 0.592 for the 2 domain, implying that the positive selection pressure probably had occurred in the PBR region of the 1 domain. Cyca-DXA24 (P0.01) was significantly correlated with the carp's resistance to Aeromonas hydrophila, while Cyca-DXA3 (P0.05), Cyca-DXA4 (P0.01), Cyca-DXA6 (P0.05) and Cyca-DXA33 (P0.05) alleles were significantly associated with the fish's susceptibility to the disease. Real-time quantitative PCR demonstrated that MHC II genes were ubiquitously expressed in ten tissues. The expression was up-regulated in kidney, down-regulated in liver and spleen during infection. Our results indicated that the MHC class II gene might play an important role in the immune reactions of C. carpio var. singguoensis.
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    • References (29)
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