GENOME-WIDE ASSOCIATION STUDY OF THERMAL TOLERANCE IN LARGE YELLOW CROAKER <i>LARIMICHTHYS CROCEA</i> BASED ON SLAF-SEQ TECHNOLOGY

CHEN Xiao-Ming; LI Jia-Kai; WANG Zhi-Yong; CAI Ming-Yi; HAN Fang; LIU Xian-De

doi:10.7541/2017.91

Volume 41 Issue 4

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ACTA HYDROBIOLOGICA SINICA > 2017 > 41(4): 735-740. > DOI: 10.7541/2017.91

CHEN Xiao-Ming, LI Jia-Kai, WANG Zhi-Yong, CAI Ming-Yi, HAN Fang, LIU Xian-De. GENOME-WIDE ASSOCIATION STUDY OF THERMAL TOLERANCE IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA BASED ON SLAF-SEQ TECHNOLOGY[J]. ACTA HYDROBIOLOGICA SINICA, 2017, 41(4): 735-740. DOI: 10.7541/2017.91

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GENOME-WIDE ASSOCIATION STUDY OF THERMAL TOLERANCE IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA BASED ON SLAF-SEQ TECHNOLOGY

Key Laboratory of Mariculture for the East China Sea, Ministry of Agriculture; Fisheries College, Jimei University, Xiamen 361021, China

Funds: Supported by the National Natural Science Foundation of China (31172397, 31402339); the New Century Excellent Talents of Fujian Province University (JA14167)

Received Date: June 21, 2016
Rev Recd Date: November 04, 2016
Published Date: June 30, 2017

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Twenty thermal-tolerant and twenty thermal-sensitive individuals of Larimichthys crocea were sequenced using specific-locus amplified fragment (SLAF-seq) technology based on Illumina HiSeq^TM2500 platform. 419211 SNPs were identified with an average read depth of 10.26× for each sample. Thirty-eight SNPs (P<2.39E–08) signifi- cantly related with thermal tolerance trait were identified according to association analysis. The SNP locations in large yellow croaker genome were identified using BLAST program, and functional genes around SNP were annotated. Twenty-six genes with known functions were discovered around 38 SNPs, which mainly regulate cell transcription, metabolism and immunity. These results provide basic information to analyze thermal-tolerant molecular mechanism and develop thermal-tolerant lines of Larimichthys crocea in the future.
- Larimichthys crocea,
- High temperature,
- SLAF-seq,
- SNP marker,
- GWAS

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GENOME-WIDE ASSOCIATION STUDY OF THERMAL TOLERANCE IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA BASED ON SLAF-SEQ TECHNOLOGY

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