Volume 42 Issue 5
Sep.  2018
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HE Jiao-Jiao, WANG Ping, FENG Jian, LOU Yu-Dong. EFFECTS OF FERMENTED SOYBEAN MEAL ON THE GROWTH AND INTESTINAL HISTOLOGY AND MICROBIOTA OF JUVENILE LARGE YELLOW CROAKER LARIMICHTHYS CROCEA[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(5): 919-928. DOI: 10.7541/2018.113
Citation: HE Jiao-Jiao, WANG Ping, FENG Jian, LOU Yu-Dong. EFFECTS OF FERMENTED SOYBEAN MEAL ON THE GROWTH AND INTESTINAL HISTOLOGY AND MICROBIOTA OF JUVENILE LARGE YELLOW CROAKER LARIMICHTHYS CROCEA[J]. ACTA HYDROBIOLOGICA SINICA, 2018, 42(5): 919-928. DOI: 10.7541/2018.113
shu

EFFECTS OF FERMENTED SOYBEAN MEAL ON THE GROWTH AND INTESTINAL HISTOLOGY AND MICROBIOTA OF JUVENILE LARGE YELLOW CROAKER LARIMICHTHYS CROCEA

  • 1.

    Zhejiang Province Key Laboratory of Marine Aquaculture Equipment and Engineering Technology, Zhejiang Ocean University, Zhoushan 316000, China

  • 2.

    School of Marine Sciences, Ningbo University, Ningbo 315211, China

Funds: Supported by the National Science Foundation of China (31602205); Public Science and Technology Research Funds Projects of Ocean (201505025); the National Natural Science Foundation of Zhejiang province (Z16E090006); the special funds projects of Ocean in Zhoushan, Zhejiang province (2015C41001); the key discipline projects for Zhejiang Ocean university of Zhejiang province (20161105)
  • Received Date: January 02, 2018
  • Rev Recd Date: March 17, 2018
  • Available Online: June 03, 2018
  • Published Date: August 31, 2018
    Graphical Abstract
    • Abstract
  • The fermented soybean meal (FSM) was used as a replacement of fish meal (FM) in a practical diet with 45% protein and 10% lipid, to investigate its effects on the growth, intestinal histology, and the structure of intestinal dominant bacteria in juvenile large yellow croaker. FM was replaced by 0, 15%, 30%, 45%, 60% and 75% of FSM (FSM0, FSM15, FSM30, FSM45, FSM60 and FSM75), respectively. The fish of 10.49 ± 0.03 g was hand-fed daily to satiation twice for 56 days. Results showed that survival was not significantly affected with all the diets (P>0.05), but in groups FSM60 and FSM75, theSR had a downward trend. In comparison with FM0, the weight gain rate (WGR) and specific growth rate (SGR) in FSM60 and FSM75 groups decreased significantly (P<0.05), while the feed conversion rate (FCR) increased significantly (P<0.05). Feed intake (FI) increased significantly in FSM60 and FSM75 groups (P<0.05), compared with that in FSM0, FSM15, FSM30 and FSM45 groups. The observed intestinal histology results showed that Mucosa thickness, Mucosafold height, Laminapropria width and Gobletcell quantity were not significantly affected in all the diets (P>0.05). Sequencing results of intestinal contents from Illumina-MiSeq high-throughput sequencing technology showed that the index of Chao1, Shannon, Simpson and Good coverage in FSM0 (TC the control), FSM45 (TB the best group in growth) and FSM75 (TW the worst group in growth) were not significantly affected (P>0.05).Firmicutes group totally dominated the bacterial communities in juvenile large yellow croaker. Paenibacillus genus was the most dominant bacteria in microbial ecosystem. Bacterial composition had certain effect on the growth of large yellow croaker: compared with TW group, the species richness of Paenibacillus and Alkaliphilus increased significantly in TB group and TC group (P<0.05). However, compared with TW group, the species richness ofEnhydrobacter in TB and Paracoccus in TC decreased significantly (P<0.05). These results suggested that the optimal dietary protein level was about 45% without negtive effects on the growth, intestinal histology, and the structure of intestinal dominant bacteria in juvenile large yellow croaker.
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