EFFECTS OF CADMIUM ON IMMUNE RESPONSES of TWO C-TYPE LECTINS IN THE FRESHWATER CRAB <i>SINOPOTAMON HENANENSE</i>

ZHAO Fang-Fang; LANG Lang; ZHANG Zuo-Bing; WANG Lan

doi:10.7541/2019.043

Volume 43 Issue 2

Feb. 2019

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ACTA HYDROBIOLOGICA SINICA > 2019 > 43(2): 348-355. > DOI: 10.7541/2019.043

ZHAO Fang-Fang, LANG Lang, ZHANG Zuo-Bing, WANG Lan. EFFECTS OF CADMIUM ON IMMUNE RESPONSES of TWO C-TYPE LECTINS IN THE FRESHWATER CRAB SINOPOTAMON HENANENSE[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(2): 348-355. DOI: 10.7541/2019.043

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EFFECTS OF CADMIUM ON IMMUNE RESPONSES of TWO C-TYPE LECTINS IN THE FRESHWATER CRAB SINOPOTAMON HENANENSE

School of Life Science, Shanxi University, Taiyuan 030006, China

Funds: Supported by the National Natural Science Foundation of China (31672293); the Shanxi Province Study Abroad Fund Key Project (2016-1)

Received Date: January 26, 2018
Rev Recd Date: October 17, 2018
Available Online: January 24, 2019
Published Date: February 28, 2019

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Abstract

Abstract

C-type lectins are a type of proteins binding to carbohydrates and important pattern recognition receptors in the innate immune system. The classical C-type lectins recognize sugars in Ca²⁺-dependent manners. It is highly acknowledged that Ca²⁺ acts as a second messenger in the cell and participates in a variety of physiological and biochemical process. Heavy metal cadmium can lead to dysregulation of cellular calcium homeostasis and interfere with intracellular Ca²⁺-related information transmission. The aim of this study was to investigate the effects of cadmium stress on the immune responses of two types of lectins, ShLec21 and ShLec23, in the freshwater crab Sinopotamon henanense. The ShLec21 and ShLec23 cDNA were cloned by RACE method, and bioinformatic analysis was carried out. In addition to their constitutive expression in selected tissues, the stimulated expression of the two C-type lectins in hepatopancreas and hemolymph after the treatment of cadmium followed by Aeromonas hydrophila infection were detected. The results showed that ShLec21 cDNA was 863 bp in length that potentinally encoded 152 amino acid residues and ShLec23 cDNA was 681 bp in length that encoded 164 amino acid residues. ShLec21 and ShLec23 clustered into two branches of invertebrates. Both of ShLec21 and ShLec23 were widely expressed in hemolymph, gill, hepatopancreas, intestine, muscle, ovary and testis with highest level in hepatopancreas. Cadmium stress had no significant effect on the expression of ShLec21 and ShLec23 in hepatopancreas and hemolymph. Bacteria A. hydrophila infection significantly down-regulated the expression of ShLec21 (P<0.05) andShLec23 (P<0.01) in the hepatopancreas, and significantly (P<0.05) reduced the expression ofShLec23 in hemolymph. However, in the course of infection with A. hydrophila after cadmium stress, the expression levels of ShLec21 (P<0.05) andShLec23 (P<0.01) were significantly up-regulated in hepatopancreas and hemolymph. The results suggest that cadmium stress could upregulate the expression ofShLec21 and ShLec23 in response to A. hydrophila infection in a certain extent.
- Sinopotamon henanense,
- C-type lectin,
- Immune response,
- Cadmium,
- Aeromonas hydrophila

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[1]	Sharon N, Lis H. History of lectins: from hemagglutinins to biological recognition molecules [J]. Glycobiology, 2004, 14(11): 53R
[2]	Wang X W, Wang J X. Diversity and multiple functions of lectins in shrimp immunity [J]. Developmental & Comparative Immunology, 2013, 39(1-2): 27—38
[3]	Wang X W, Wang J X. Pattern recognition receptors acting in innate immune system of shrimp against pathogen infections [J]. Fish & Shellfish Immunology, 2013, 34(4): 981—989
[4]	Weis W I, Taylor M E, Drickamer K. The C-type lectin superfamily in the immune system [J]. Immunological Reviews, 1998, 163(1): 19—34
[5]	Chen D D, Meng X L, Xu J P, et al. PcLT, a novel C-type lectin from Procambarus clarkii, is involved in the innate defense against Vibrio alginolyticus and WSSV [J]. Developmental & Comparative Immunology, 2013, 39(3): 255—264
[6]	Junkunlo K, Prachumwat A, Tangprasittipap A, et al. A novel lectin domain-containing protein (LvCTLD) associated with response of the whiteleg shrimp Penaeus (Litopenaeus) vannamei to yellow head virus (YHV) [J]. Developmental & Comparative Immunology, 2012, 37(3-4): 334—341
[7]	Luo T, Yang H, Li F, et al. Purification, characterization and cDNA cloning of a novel lipopolysaccharide-binding lectin from the shrimp Penaeus monodon [J]. Developmental & Comparative Immunology, 2006, 30(7): 607—617
[8]	Costa F H, Valenca N S, Silva A R, et al. Cloning and molecular modeling of Litopenaeus vannamei (Penaeidae) C-type lectin homologs with mutated mannose binding domain-2 [J]. Genetics & Molecular Research, 2011, 10(2): 650—664
[9]	Huang X, Li W, Jin M, et al. Single CRD containing lectin from Macrobrachium rosenbergii (MrLec) participates in innate immunity against pathogen infections [J]. Fish & Shellfish Immunology, 2016, 51: 282—290
[10]	Jin X K, Li W W, Cheng L, et al. Two novel short C-type lectin from Chinese mitten crab, Eriocheir sinensis, are induced in response to LPS challenged [J]. Fish & Shellfish Immunology, 2012, 33(5): 1149—1158
[11]	Fang Z Y, Li D, Li X J, et al. A single CRD C-type lectin from Eriocheir sinensis (EsLecB) with microbial-binding, antibacterial prophenoloxidase activation and hem-encapsulation activities [J]. Fish & Shellfish Immunology, 2016, 50: 175—190
[12]	Kong H J, Park E M, Nam B H, et al. A C-type lectin like-domain (CTLD)-containing protein (PtLP) from the swimming crab Portunus trituberculatus [J]. Fish & Shellfish Immunology, 2008, 25(3): 311—314
[13]	Guo X N, Jin X K, Li S, et al. A novel C-type lectin from Eriocheir sinensis functions as a pattern recognition receptor with antibacterial activity [J]. Fish & Shellfish Immunology, 2013, 35(5): 1554—1565
[14]	Guo H Z, Zou P F, Fu J P, et al. Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis [J]. Fish & Shellfish Immunology, 2011, 30(2): 515—524
[15]	段利朋, 黄贝, 周立红, 等. 拟穴青蟹两种新C-型凝集素基因的克隆与表达分析. 水生生物学报, 2015, 39(2): 321—330 Duan L P, Huang B, Zhou L H, et al. Molecular cloning, characterization and expression of two novel lectins in mud crab, Scylla paramamosain [J]. Acta Hydrobiologica Sinica, 2015, 39(2): 321—330
[16]	Kumar S R, Agrawal M, Marshall F. Heavy metal contamination of soil and vegetables in suburban areas of Varanasi, India [J]. Ecotoxicology & Environmental Safety, 2007, 66(2): 258—266
[17]	Valko M, Izakovic M, Mazur M, et al. Role of oxygen radicals in DNA damage and cancer incidence [J]. Molecular and Cellular Biochemistry, 2004, 266(1/2): 37—56
[18]	Zhan Q, Tang M. Research advances on apoptosis caused by quantum dots [J]. Biological Trace Element Research, 2014, 161(1): 3—12
[19]	侯宇华, 李娜, 李丹, 等. 镉对河南华溪蟹副性腺抗氧化酶活性及脂质、蛋白质和DNA的影响. 水生生物学报, 2015, 39(3): 621—626 Hou Y H, Li N, Li D, et al. Bioaccumulation of cadmium and its effects on antioxidant enzyme activities, lipid, protein and dna in male accessory gland of the freshwater crab Sinopotamon henanense [J]. Acta Hydrobiologica Sinica, 2015, 39(3): 621—626
[20]	杨健, 刘冬梅, 何永吉, 等. 镉对河南华溪蟹卵黄磷蛋白在卵巢中表达含量的影响及ELISA法的建立. 水生生物学报, 2015, 39(2): 287—293 Yang J, Liu D M, He Y J, et al. Established of elisa method of vitellin from freshwater crab Sinopotamon henanense and effect of cadmium on vitellin accumulation in ovary [J]. Acta Hydrobiologica Sinica, 2015, 39(2): 287—293
[21]	王茜, 郭鹄飞, 王兰. 镉对大型溞摄食能力和相关生理指标的影响. 水生生物学报, 2018, 42(3): 616—621 Wang Q, Guo H F, Wang L. Effect of cadmium on the feeding capacity and physiological status of Daphnia magna [J]. Acta Hydrobiologica Sinica, 2018, 42(3): 616—621
[22]	Qin Q, Qin S, Wang L, et al. Immune responses and ultrastructural changes of hemocytes in freshwater crab Sinopotamon henanense exposed to elevated cadmium [J]. Aquatic Toxicology, 2012, (106–107): 140—146
[23]	Lang L, Zhang Z, Jing W, et al. Identification of a novel toll gene (Shtoll3) from the freshwater crab Sinopotamon henanense and its expression pattern changes in response to cadmium followed by Aeromonas hydrophila infection [J]. Fish & Shellfish Immunology, 2017, 71: 177—190
[24]	Li L, Zhao C P, Li H, et al. Establishment of the plasmid standard curve generation method for absolute quantification PCR [J]. Journal of Agricultural Biotechnology, 2011, 19(6): 1157—1162
[25]	Lang X, Wang L, Zhang Z. Stability evaluation of reference genes for real-time PCR in zebrafish (Danio rerio) exposed to cadmium chloride and subsequently infected by bacteria Aeromonas hydrophila [J]. Aquatic Toxicology, 2016, 170: 240—250
[26]	Robinson M J, Sancho D, Slack E C, et al. Myeloid C-type lectins in innate immunity [J]. Nature Immunology, 2006, 7(12): 1258—1265
[27]	Runsaeng P, Thepnarong S, Rattanaporn O, et al. Cloning and the mRNA expression of a C-type lectin with one carbohydrate recognition domain from Fenneropenaeus merguiensis in response to pathogenic inoculation [J]. Molecular & Cellular Probes, 2015, 29(6): 365—375
[28]	Ren Q, Li M, Du J, et al. Immune response of four dual-CRD C-type lectins to microbial challenges in giant freshwater prawn Macrobrachium rosenbergii [J]. Fish & Shellfish Immunology, 2012, 33(2): 155—167
[29]	Zelensky A N, Gready J E. The C-type lectin-like domain superfamily [J]. FEBS Journal, 2005, 272(24): 6179—217
[30]	Wang L, Wang L, Huang M, et al. The immune role of C-type lectins in molluscs [J]. Invertebrate Survival Journal, 2011, 8(2): 241—246
[31]	Li M, Li C, Ma C, et al. Identification of a C-type lectin with antiviral and antibacterial activity from pacific white shrimp Litopenaeus vannamei [J]. Developmental & Comparative Immunology, 2014, 46(2): 231—240
[32]	Liu Y C, Li F H, Dong B, et al. Molecular cloning, characterization and expression analysis of a putative C-type lectin (Fclectin) gene in Chinese shrimp Fenneropenaeus chinensis [J]. Molecular Immunology, 2007, 44(4): 598—607
[33]	Huang Y, Huang X, Wang Z, et al. Function of two novel single-CRD containing C-type lectins in innate immunity from Eriocheir sinensis [J]. Fish & Shellfish Immunology, 2014, 37(2): 313—321
[34]	Ma T H, Tiu S H, He J G, et al. Molecular cloning of a C-type lectin (LvLT) from the shrimp Litopenaeus vannamei: early gene down-regulation after WSSV infection [J]. Fish & Shellfish Immunology, 2007, 23(2): 430—437

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EFFECTS OF CADMIUM ON IMMUNE RESPONSES of TWO C-TYPE LECTINS IN THE FRESHWATER CRAB SINOPOTAMON HENANENSE

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