CHAIN SPECIFICITY TRANSCRIPTOME ANALYSIS IN DIFFERENT TEMPERATURES CONDITIONED OF <i>YERSINIA RUCKERI</i>

LIU Tao; WEI Wen-Yan; LIU Jia-Xing; YANG Ma; XIE Heng; HE Sheng-Yu; YANG Qian; WANG Kai-Yu

doi:10.7541/2019.115

Volume 43 Issue 5

Aug. 2019

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ACTA HYDROBIOLOGICA SINICA > 2019 > 43(5): 969-976. > DOI: 10.7541/2019.115

LIU Tao, WEI Wen-Yan, LIU Jia-Xing, YANG Ma, XIE Heng, HE Sheng-Yu, YANG Qian, WANG Kai-Yu. CHAIN SPECIFICITY TRANSCRIPTOME ANALYSIS IN DIFFERENT TEMPERATURES CONDITIONED OF YERSINIA RUCKERI[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(5): 969-976. DOI: 10.7541/2019.115

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CHAIN SPECIFICITY TRANSCRIPTOME ANALYSIS IN DIFFERENT TEMPERATURES CONDITIONED OF YERSINIA RUCKERI

1.
Fisheries Department of Sichuan Agricultural University, Chengdu 611130, China
2.
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China
3.
Institute of fisheries of Chengdu Agriculture and Forestry Academy, Chengdu 610000, China

Funds: Supported by Jointly by Sichuan Technology Support Planning at Sichuan Agricultural University (IRT0848); the Scientific Research Innovation Project of Chengdu Academy of Agricultural and Forestry Sciences (2017-Y2500W-16)

Received Date: July 11, 2018
Rev Recd Date: January 20, 2019
Available Online: June 03, 2019
Published Date: August 31, 2019

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Abstract

To investigate the effect of temperature on transcripts and relative mRNA levels, high-throughput RNA sequencing were used for Yersinia ruckeri grown at 28℃ and 37℃. After the quality control, all differentially regulated genes were enriched and annotated by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and verified by Rockhopper software. The results showed that 173 differentially expressed genes were identified (P-value<0.05), including 58 up-regulated genes mainly enriched in several KEGG pathways: phosphotransferase system, starch and sucrose metabolism, galactose metabolism and other glycan degradation; and 115 down-regulated genes mainly enriched in several KEGG pathways: two-component system, thiamine metabolism and flagellar assembly. In summary, the SC09 had lower motility and weaker glucose metabolism at temperature of 37℃. The compensatory overexpression of some specific carbohydrate metabolism might play important roles for the survival of this bacterial under high temperature. This study provides an important basis for further research on the molecular mechanisms of SC09 in different temperatures.
- Yersinia ruckeri,
- RNA-seq,
- Differential expression analysis,
- Kyoto Encyclopedia of Genes and Genomes,
- Flagellar gene cluster

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CHAIN SPECIFICITY TRANSCRIPTOME ANALYSIS IN DIFFERENT TEMPERATURES CONDITIONED OF YERSINIA RUCKERI

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