INHIBITORY EFFECT OF <i>SCENEDESMUS</i> SP. ON <i>MICROCYSTIS AERUGINOSA</i> AND ITS EVALUATION

SONG Chan-Yuan; BAI Fang; LI Tian-Li; SONG Li-Rong

doi:10.7541/2023.2022.0042

Volume 46 Issue 12

Dec. 2022

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ACTA HYDROBIOLOGICA SINICA > 2022 > 46(12): 1916-1923. > DOI: 10.7541/2023.2022.0042

SONG Chan-Yuan, BAI Fang, LI Tian-Li, SONG Li-Rong. INHIBITORY EFFECT OF SCENEDESMUS SP. ON MICROCYSTIS AERUGINOSA AND ITS EVALUATION[J]. ACTA HYDROBIOLOGICA SINICA, 2022, 46(12): 1916-1923. DOI: 10.7541/2023.2022.0042

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INHIBITORY EFFECT OF SCENEDESMUS SP. ON MICROCYSTIS AERUGINOSA AND ITS EVALUATION

1.
Dalian Ocean University, Dalian 116023, China
2.
State Key Laboratory of Fresh Water Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Funds: Supported by a Grant from the National Key Research and Development of the People’s Republic of China (2017YFE0125700)

Received Date: January 25, 2022
Rev Recd Date: March 31, 2022
Available Online: April 24, 2022
Published Date: December 14, 2022

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Abstract

Abstract

Cyanobacterial harmful algal blooms are a global environmental problem. Microcystis, which can produce microcystin, is one of the most pervasive bloom forming cyanobacteria in freshwater ecosystems. Blooms of Microcystis have adverse effects on lake ecology, and may effect human health, so it is important to control Microcystis blooms. In order to investigate the inhibitory effect of green algae on Microcystis aeruginosa based on allelopathy among different algae strains, we screened potential green algae to control M. aeruginosa blooms. One strain which has allelochemicals production properties was screened from 34 strains of green algae. The results showed that the spent medium of Scenedesmus sp. FACHB-1229 had the highest inhibition rate, for M. aeruginosa FACHB-3550 was 53.95% and for FACHB-905 was 48.85%, respectively. Result of gas chromatography mass spectrometry (GC-MS) indicated that 1,2-Benzenedicarboxylic acid, bis (2-methoxyethyl) ester might be the allelochemical substances. In addition, we also measured growth rates, high ammonia nitrogen tolerance and photosynthetic oxygen evolution rates of the strain. The results indicated that Scenedesmus sp. FACHB-1229 had high specific growth rate of (0.38±0.06)/d. It also had the highest photosynthetic oxygen evolution rates of (229.91±10.49) μmol O₂/(mg Chl.a·h). The ammonia nitrogen tolerance of Scenedesmus sp. FACHB-1229 was significantly higher than other strains, its growth rate was (0.30±0.08)/d when the ammonia nitrogen concentration reached up to 1888.60 mg/L. Moreover, the proportion of FACHB-1229 was consistently increased when co-cultured with FACHB-3550. In summary, FACHB-1229 has the superiority in interspecies competition with M. aeruginosa, its spent medium can inhibit the growth of M. aeruginosa, and it also has high ammonia nitrogen tolerance and photosynthetic oxygen evolution rate. Therefore, this study has provided the theory and application basis in prevention and control of cyanobacterial blooms using interspecific competition among algae. FACHB-1229 has the potential to control harmful algae through biological methods, it can grow rapidly in aquaculture pond or other polluted water bodies with high ammonia nitrogen concentration, while it can inhibit M. aeruginosa through allelopathy and provide dissolved oxygen at the same time.
- Allelopathy,
- Biological control of cyanobacteria,
- Interspecific competition,
- Microcystis aeruginosa,
- Scenedesmus sp.

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INHIBITORY EFFECT OF SCENEDESMUS SP. ON MICROCYSTIS AERUGINOSA AND ITS EVALUATION

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