DIETARY AFLATOXIN B<sub>1 </sub>ON GROWTH, ANTIOXIDANT CAPACITY AND HISTOLOGY IN HEPATOPANCREAS OF JUVENILE RED SWAMP CRAWFISH (<i>PROCAMBARUS CLARKII</i>)

HUANG Ying; GUO Ya-Zhe; LOU Ge-Ge; MAN Zhou; GE Ru-Xiang; ZHU Xiao-Ming; XIANG Yong; CHEN Xin-Hua

doi:10.7541/2023.2022.0260

Volume 47 Issue 2

Jan. 2023

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ACTA HYDROBIOLOGICA SINICA > 2023 > 47(2): 279-289. > DOI: 10.7541/2023.2022.0260

HUANG Ying, GUO Ya-Zhe, LOU Ge-Ge, MAN Zhou, GE Ru-Xiang, ZHU Xiao-Ming, XIANG Yong, CHEN Xin-Hua. DIETARY AFLATOXIN B₁ON GROWTH, ANTIOXIDANT CAPACITY AND HISTOLOGY IN HEPATOPANCREAS OF JUVENILE RED SWAMP CRAWFISH (PROCAMBARUS CLARKII)[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 279-289. DOI: 10.7541/2023.2022.0260

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DIETARY AFLATOXIN B₁ON GROWTH, ANTIOXIDANT CAPACITY AND HISTOLOGY IN HEPATOPANCREAS OF JUVENILE RED SWAMP CRAWFISH (PROCAMBARUS CLARKII)

1.
Key Laboratory of Marine Biotechnology of Fujian Province, College of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
2.
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

Funds: Supported by the National Key R & D Program of China (2019YFD0900200); Science and Technology Innovation Foundation of Fujian Agriculture and Forestry University (CXZX2019064S); Cooperative Project with DSM Vitamins (Shanghai) Limited (KH210114A); Natural Science Foundation of Fujian Province (2016J01093))

Received Date: June 23, 2022
Rev Recd Date: August 10, 2022
Available Online: August 21, 2022
Published Date: February 14, 2023

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Abstract

Abstract

To evaluate the effects of dietary aflatoxin B₁ (AFB₁) on growth performance, feed efficiency, and histological changes in juvenile red swamp crayfish (Procambarus clarkii), crayfish with initial body weight of [(0.382±0.005) g] were fed with four diets containing 0, 10, 100 and 1000 μg/kg AFB₁ for a 42-day trial. Significant lower survival rate (SR), feeding rate (FR), final body weight (FBW), specific growth rate (SGR) and feed efficiency (FE) were observed in 100 μg/kg and 1000 μg/kg AFB₁ group, while no significant differences were found in 10 μg/kg group compared to control group. There were no significant differences in alkaline phosphatase (AKP), alanine aminotransferase (ALT), aspartate, aminotransferase (AST), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) and malondialdehyde (MDA) in hepatopancreas between the control and 10 μg/kg AFB₁ group, while significant decrease in superoxide dismutase (SOD) was observed in 10 μg/kg AFB₁ group. AFB₁ above 100 μg/kg significantly impacted activities of the aforementioned hepatopancreas enzymes. Slight histological changes were identified in hepatopancreas of 10 μg/kg AFB₁ group. However, severe lesions of hepatopancreas were found in 100 μg/kg and 1000 μg/kg AFB₁ group, which showed decreased numbers of R-cells and increased numbers of B-cells. The ultrastructural results showed that with the increase of AFB₁ concentration, hepatopancreas demonstrated swelling mitochondria, expanded rough endoplasmic reticulum, increased cytoplasmic vesicles and larger lipid droplets. No AFB₁ residues were detected in whole body when crayfish were fed with AFB₁ up to 100 μg/kg, however, tiny dose (1.65 μg/kg) of AFB₁ were detected in the group with 1000 μg/kg AFB₁, which was below the safety limitation of FDA. In summary, dietary AFB₁ above 10 μg/kg had an adverse effect on juvenile crayfish, which indicated that red swamp crayfish was a sensitive species to AFB₁.
- Aflatoxin B₁,
- Growth,
- Antioxidant ability,
- Hepatopancreas,
- Accumulation,
- Procambarus clarkii

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DIETARY AFLATOXIN B₁ON GROWTH, ANTIOXIDANT CAPACITY AND HISTOLOGY IN HEPATOPANCREAS OF JUVENILE RED SWAMP CRAWFISH (PROCAMBARUS CLARKII)

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DIETARY AFLATOXIN B1 ON GROWTH, ANTIOXIDANT CAPACITY AND HISTOLOGY IN HEPATOPANCREAS OF JUVENILE RED SWAMP CRAWFISH (PROCAMBARUS CLARKII)

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DIETARY AFLATOXIN B₁ON GROWTH, ANTIOXIDANT CAPACITY AND HISTOLOGY IN HEPATOPANCREAS OF JUVENILE RED SWAMP CRAWFISH (PROCAMBARUS CLARKII)