Volume 47 Issue 3
Mar.  2023
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LEI Yao, ZHOU Chun-Hua, OUYANG Shan, WU Xiao-Ping. DIFFERENCES IN ENVIRONMENTAL DNA MONITORING OF FRESHWATER MUSSELS FROM DIFFERENT ENVIRONMENTAL SAMPLE TYPES[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(3): 412-423. DOI: 10.7541/2023.2022.0266
Citation: LEI Yao, ZHOU Chun-Hua, OUYANG Shan, WU Xiao-Ping. DIFFERENCES IN ENVIRONMENTAL DNA MONITORING OF FRESHWATER MUSSELS FROM DIFFERENT ENVIRONMENTAL SAMPLE TYPES[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(3): 412-423. DOI: 10.7541/2023.2022.0266
shu

DIFFERENCES IN ENVIRONMENTAL DNA MONITORING OF FRESHWATER MUSSELS FROM DIFFERENT ENVIRONMENTAL SAMPLE TYPES

  • 1.

    College of Life Science; Nanchang University, Nanchang 330031, China

  • 2.

    Ministry of Education, Key Laboratory of Environment and Resource Utilization of Poyang Lake, Nanchang University, Nanchang 330031, China

  • 3.

    Jiangxi Province Key Laboratory of Watershed Ecosystem Change and Biodiversity, Nanchang 330031, China

Funds: Supported by the National Key Research and Development Program of China(2018YFD0900801); the Special Fund for Postgraduate Innovation of Jiangxi Province in 2021 (YC2021-S038)
  • Received Date: June 28, 2022
  • Rev Recd Date: August 23, 2022
  • Available Online: September 19, 2022
  • Published Date: March 14, 2023
    Graphical Abstract
    • Abstract
  • In order to clarify how different environmental sample types affect the detectability of mussel species when using environmental DNA metabarcoding technology, surface water, bottom water and sediment were collected in Poyang Lake in winter and spring of 2021 for environmental DNA metabarcoding analysis, and then combined with traditional methods for collection and verification. A total of 33 species of mussels from Poyang Lake were detected by environmental DNA metabarcoding technology, while 18 species were collected by traditional methods. All the species collected by traditional methods could be detected by environmental DNA metabarcoding technology. The number of mussel species annotated in surface water and bottom water was respectively higher than that in sediment, and the mussel species annotated in surface water and bottom water completely covered sediment, respectively. There was no significant seasonal difference in α diversity level of mussels based on environmental DNA metabarcoding, but significant seasonal difference in β diversity level of mussels. The mussel diversity in both surface and bottom water was significantly respectively higher than that in sediment samples. The Beta diversity analysis also showed significant differences between water samples (surface and bottom water respectively) and sediment samples. But there were no significant differences in the diversity and community structure between surface and bottom water. Water depth (WD), depth of Secchi disk (SD), water temperature (WT) and total nitrogen (TN) significantly affected the community structure of mussels in Poyang Lake. Environmental DNA metabarcoding is feasible in monitoring mussel freshwater diversity, and water sampling is better than sediment sampling. There is no significant difference between surface water and bottom water.
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