| Citation: |
JIANG You-Bo, SUN Rui-Dong, XIE Yan, CHENG Yi, CHEN Jian, LI Pei, WANG Zhong-Wei, XIONG Yang, MEI Jie. PRODUCTION OF YY SUPER-MALE AND XY ALL-MALE PSEUDOBAGRUS USSURIENSIS BY SEX CONTROL TECHNOLOGY[J]. ACTA HYDROBIOLOGICA SINICA. DOI: 10.7541/2025.2024.0407
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In this study, we investigated the effects of various doses and treatment durations of 17β-estradiol (E2) on the growth, survival rate, and sex ratio of P. ussuriensis. From 10 to 70dph (days post-hatching), feeding P. ussuriensis diets containing 10, 50, and 100 mg/kg E2 resulted in a 100% feminization rate. However, the growth and survival rates decreased with increasing E2 concentration, and all fish in the group fed with 150 mg/kg E2 died. Overall, 10 mg/kg E2 treatment from 10 to 40dph was found to be the optimal approach for the feminization of P. ussuriensis. The expression levels of male key sex differentiation-related genes, such as dmrt1, amh, and cyp17a1, were significantly lower in XY females than that of XY males. The expression levels of female key sex differentiation-related genes including cyp19a1a, zar1, and gdf9 in XY females were consistent with those in XX females and were significantly higher than that in XY males. Additionally, whole-genome resequencing revealed 3777645 single nucleotide polymorphisms (SNPs) and 1287509 insertions/deletions (Indels) in 15 female and 15 male P. ussuriensis. Among these, 99601 were identified as sex-linked SNPs and 27614 as sex-linked Indels. The sex-linked SNPs were enriched in the 6.84 and 23.82 Mb regions of chromosome 8. X and Y chromosome specific molecular marker were developed based on sex-linked Indels, which proved effective for the sex identification in P. ussuriensis. These markers were well applied to wild populations from the Heilongjiang River as well as farmed populations in Henan and Hubei Province. YY super-male were identified from the offsprings of XY male mating with XY female. Subsequently, using YY super-male P. ussuriensis as the male parent, an all-male population was bred with a 100% male ratio. The all-male population at one-year old was 1.54 times heavier than the mixed-sex population. In summary, 10 mg/kg E2 treatment from 10 to 40dph is the optimal approach for the feminization of P. ussuriensis. The sex-linked molecular markers developed by whole-genome resequencing can effectively identify the genotype of P. ussuriensis and help produce YY super-male fish. XY all-male P. ussuriensis had the characteristics of uniform size and fast growth performance which will be more popular among farmers.
| [1] |
梅洁, 桂建芳. 鱼类性别异形和性别决定的遗传基础及其生物技术操控 [J]. 中国科学: 生命科学, 2014, 44(12): 1198-1212.]
Mei J, Gui J F. Genetic basis and biotechnological manipulation of sexual dimorphism and sex determination in fish [J]. Science China Life Sciences, 2014, 44(12): 1198-1212. [
|
| [2] |
魏梦雅, 王思梦, 宁静, 等. 乌苏里拟鲿igf基因克隆及其与生长性状关联分析 [J]. 水产科学, 2022, 41(5): 738-748.]
Wei M Y, Wang S M, Ning J, et al. Cloning and association analysis with growth traits of igf gene in Ussuri catfish Pseudobagrus ussuriensis [J]. Fisheries Science, 2022, 41(5): 738-748. [
|
| [3] |
Pan Z J, Zhu C K, Wang H, et al. Gonadal morphogenesis and sex differentiation in cultured Ussuri catfish Tachysurus ussuriensis [J]. Journal of Fish Biology, 2017, 91(3): 866-879. doi: 10.1111/jfb.13388
|
| [4] |
Pandian T J, Sheela S G. Hormonal induction of sex reversal in fish [J]. Aquaculture, 1995, 138(1-4): 1-22. doi: 10.1016/0044-8486(95)01075-0
|
| [5] |
Valdivia K, Jouanno E, Volff J N, et al. High temperature increases the masculinization rate of the all-female (XX) rainbow trout “Mal” population [J]. PLoS One, 2014, 9(12): e113355. doi: 10.1371/journal.pone.0113355
|
| [6] |
Piferrer F, Anastasiadi D. Do the offspring of sex reversals have higher sensitivity to environmental perturbations [J]? Sexual Development, 2021, 15(1-3): 134-147.
|
| [7] |
徐思琪, 张世勇, 段永强, 等. 17β-雌二醇诱导斑点叉尾鮰雌性化研究 [J]. 水生生物学报, 2022, 46(11): 1668-1674.] doi: 10.7541/2022.2021.0254
Xu S Q, Zhang S Y, Duan Y Q, et al. Feminization of channel catfish induced by 17β-estradiol [J]. Acta Hydrobiologica Sinica, 2022, 46(11): 1668-1674. [ doi: 10.7541/2022.2021.0254
|
| [8] |
刘汉勤, 崔书勤, 侯昌春, 等. 从XY雌鱼雌核发育产生YY超雄黄颡鱼 [J]. 水生生物学报, 2007, 31(5): 718-725.] doi: 10.3321/j.issn:1000-3207.2007.05.018
Liu H Q, Cui S Q, Hou C C, et al. YY super-male generated gynogenetically from XY female in Pelteobagrus fulvidraco (Richardson) [J]. Acta Hydrobiologica Sinica, 2007, 31(5): 718-725. [ doi: 10.3321/j.issn:1000-3207.2007.05.018
|
| [9] |
de Bem J C, Fontanetti C S, Senhorini J A, et al. Effectiveness of estradiol valerate on sex reversion in Astyanax altiparanae (Characiformes, Characidae) [J]. Brazilian Archives of Biology and Technology, 2012, 55(2): 283-290. doi: 10.1590/S1516-89132012000200015
|
| [10] |
王成龙, 关文志, 李永强, 等. 17β-雌二醇诱导黄颡鱼雌性化的研究 [J]. 南方水产科学, 2020, 16(3): 25-30.] doi: 10.12131/20200001
Wang C L, Guan W Z, Li Y Q, et al. Study on 17β-estradiol induced feminization of Pelteobagrus fulvidraco [J]. South China Fisheries Science, 2020, 16(3): 25-30. [ doi: 10.12131/20200001
|
| [11] |
Piferrer F. Endocrine sex control strategies for the feminization of teleost fish [J]. Aquaculture, 2001, 197(1/2/3/4): 229-281.
|
| [12] |
陶彬彬, 胡炜. 鱼类性别控制育种研究进展 [J]. 中国农业科技导报, 2022, 24(2): 1-10.]
Tao B B, Hu W. Research progress on sex control breeding of fish [J]. Journal of Agricultural Science and Technology, 2022, 24(2): 1-10. [
|
| [13] |
刘洋, 陈松林. 鱼类性别特异分子标记筛选及分子性控育种研究现状与展望 [J]. 水产学报, 2023, 47(11): 34-43.]
Liu Y, Chen S L. Fish sex-specific molecular marker screening and marker-assisted sex-controlled breeding: history, progress and prospect [J]. Journal of Fisheries of China, 2023, 47(11): 34-43. [
|
| [14] |
Zhou Y L, Wu J J, Wang Z W, et al. Identification of sex-specific markers and heterogametic XX/XY sex determination system by 2b-RAD sequencing in redtail catfish (Mystus wyckioides) [J]. Aquaculture Research, 2019, 50(8): 2251-2266. doi: 10.1111/are.14106
|
| [15] |
Han C, Zhu Q Y, Lu H M, et al. Screening and characterization of sex-specific markers developed by a simple NGS method in mandarin fish (Siniperca chuatsi) [J]. Aquaculture, 2020(527): 735495.
|
| [16] |
Liao Q, Gong G R, Wang J Q, et al. Identification of sex-linked codominant markers and development of a rapid LAMP-based genetic sex identification method in channel catfish (Ictalurus punctatus) [J]. Aquaculture, 2023(572): 739556.
|
| [17] |
Xiong Y, Wang X, Sun R D, et al. Administration of Arginine Vasotocin and modified Isotocin improve artificial propagation and post-spawning survival of female yellow catfish [J]. Aquaculture, 2024(587): 740849.
|
| [18] |
Capel B. Vertebrate sex determination: evolutionary plasticity of a fundamental switch [J]. Nature Reviews Genetics, 2017, 18(11): 675-689. doi: 10.1038/nrg.2017.60
|
| [19] |
熊阳, 王帅, 郭稳杰, 等. 不同动物饵料对YY超雄黄颡鱼性腺发育的影响 [J]. 水产学报, 2020, 44(2): 245-252.]
Xiong Y, Wang S, Guo W J, et al. Effects of different animal baits on the gonad development of YY super-male yellow catfish (Pelteobagrus fulvidruco) [J]. Journal of Fisheries of China, 2020, 44(2): 245-252. [
|
| [20] |
Dong R R, Yang S J, Feng R J, et al. Complete feminization of catfish by feeding Limnodilus, an annelid worm collected in contaminated streams [J]. Environmental Research, 2014(133): 371-379.
|
| [21] |
姚道霞. 黄颡鱼性分化及激素诱导性转化研究 [D]. 哈尔滨: 东北林业大学, 2007: 21-40.]
Yao D X. Sex differentiation and hormonal sex reversal of Pelteobagrus fulvidraco [D]. Harbin: Northeast Forestry University, 2007: 21-40. [
|
| [22] |
胡景琦. 人工诱导瓦氏黄颡鱼性别逆转及雌核发育研究 [D]. 武汉: 华中农业大学, 2024: 23-28.]
Hu J Q. Study on artificially induced sexual-reversal and gynogenesis in Pelteobagrus vachelli [D]. Wuhan: Huazhong Agricultural University, 2024: 23-28. [
|
| [23] |
Xiong Y, Jiang Y B, Sun R D, et al. Characterization of early gonadal differentiation and estrogen-induced feminization in Chinese longsnout catfish (Leiocassis longirostris) [J]. Aquaculture Reports, 2024(39): 102373.
|
| [24] |
Wenger M, Sattler U, Goldschmidt-Clermont E, et al. 17Beta-estradiol affects the response of complement components and survival of rainbow trout (Oncorhynchus mykiss) challenged by bacterial infection [J]. Fish & Shellfish Immunology, 2011, 31(1): 90-97.
|
| [25] |
王凌宇, 齐飘飘, 陈敏, 等. 性类固醇激素对黄颡鱼雌雄生长二态性的影响 [J]. 水生生物学报, 2020, 44(2): 379-388.] doi: 10.7541/2020.046
Wang L Y, Qi P P, Chen M, et al. Effects of sex steroid hormones on sexual size dimorphism in yellow catfish (Tachysurus fulvidraco) [J]. Acta Hydrobiologica Sinica, 2020, 44(2): 379-388. [ doi: 10.7541/2020.046
|
| [26] |
Ferguson-Smith M. The evolution of sex chromosomes and sex determination in vertebrates and the key role of DMRT1 [J]. Sexual Development, 2007, 1(1): 2-11. doi: 10.1159/000096234
|
| [27] |
Lin Q, Mei J, Li Z, et al. Distinct and cooperative roles of amh and dmrt1 in self-renewal and differentiation of male germ cells in zebrafish [J]. Genetics, 2017, 207(3): 1007-1022. doi: 10.1534/genetics.117.300274
|
| [28] |
Chen W T, Liu L, Ge W. Expression analysis of growth differentiation factor 9 (Gdf9/gdf9), anti-müllerian hormone (Amh/amh) and aromatase (Cyp19a1a/cyp19a1a) during gonadal differentiation of the zebrafish, Danio rerio [J]. Biology of Reproduction, 2017, 96(2): 401-413. doi: 10.1095/biolreprod.116.144964
|
| [29] |
Guiguen Y, Fostier A, Piferrer F, et al. Ovarian aromatase and estrogens: a pivotal role for gonadal sex differentiation and sex change in fish [J]. General and Comparative Endocrinology, 2010, 165(3): 352-366. doi: 10.1016/j.ygcen.2009.03.002
|
| [30] |
Miao L, Yuan Y, Cheng F, et al. Translation repression by maternal RNA binding protein Zar1 is essential for early oogenesis in zebrafish [J]. Development, 2017, 144(1): 128-138.
|
| [31] |
Pan Z J, Zhu C K, Chang G L, et al. Differential expression analysis and identification of sex-related genes by gonad transcriptome sequencing in estradiol-treated and non-treated Ussuri catfish Pseudobagrus ussuriensis [J]. Fish Physiology and Biochemistry, 2021, 47(2): 565-581. doi: 10.1007/s10695-021-00932-x
|
| [32] |
Bentley D R. Whole-genome re-sequencing [J]. Current Opinion in Genetics & Development, 2006, 16(6): 545-552.
|
| [33] |
张俊杰, 赵瑞阳, 蒋丽, 等. 白斑狗鱼雌、雄基因组混池重测序研究 [J]. 淡水渔业, 2020, 50(3): 17-25.] doi: 10.3969/j.issn.1000-6907.2020.03.003
Zhang J J, Zhao R Y, Jiang L, et al. Resequencing analysis of the female and male mixed pool of Esox lucius [J]. Freshwater Fisheries, 2020, 50(3): 17-25. [ doi: 10.3969/j.issn.1000-6907.2020.03.003
|
| [34] |
Zheng S Q, Wang X S, Zhang S, et al. Screening and characterization of sex-linked DNA markers and marker-assisted selection in the Southern catfish (Silurus meridionalis) [J]. Aquaculture, 2020(517): 734783.
|
| [35] |
Pan Z J, Li X Y, Zhou F J, et al. Identification of sex-specific markers reveals male heterogametic sex determination in Pseudobagrus ussuriensis [J]. Marine Biotechnology, 2015, 17(4): 441-451. doi: 10.1007/s10126-015-9631-2
|
| [36] |
Zhu C K, Liu H Y, Cheng L, et al. Identification of sex-specific sequences through 2b-RAD sequencing in Pseudobagrus ussuriensis [J]. Aquaculture, 2021(539): 736639.
|
| [37] |
Pruginin Y, Rothbard S, Wohlfarth G, et al. All-male broods of Tilapia nilotica× T. aurea hybrids [J]. Aquaculture, 1975, 6(1): 11-21. doi: 10.1016/0044-8486(75)90086-1
|
| [38] |
Liu H, Guan B, Xu J, et al. Genetic manipulation of sex ratio for the large-scale breeding of YY super-male and XY all-male yellow catfish (Pelteobagrus fulvidraco (Richardson) [J]. Marine Biotechnology, 2013, 15(3): 321-328. doi: 10.1007/s10126-012-9487-7
|
| [39] |
罗非鱼“粤闽1号” [J]. 中国水产, 2021(4): 92-96.]
Tilapia “YueMin No. 1” [J]. China Fisheries, 2021(4): 92-96. [
|
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