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GAO Ming-Yue, CHU Xin, LIU Si-Yu, YAO Jia-Yun, LIN Ling-Yun, SHEN Jin-Yu, PAN Xiao-Yi. EVALUATION OF IMMUNE EFFECT ON ATTENUATED STRAIN OF LARGEMOUTH BASS RANAVIRUS[J]. ACTA HYDROBIOLOGICA SINICA. DOI: 10.7541/2025.2024.0447
Citation: GAO Ming-Yue, CHU Xin, LIU Si-Yu, YAO Jia-Yun, LIN Ling-Yun, SHEN Jin-Yu, PAN Xiao-Yi. EVALUATION OF IMMUNE EFFECT ON ATTENUATED STRAIN OF LARGEMOUTH BASS RANAVIRUS[J]. ACTA HYDROBIOLOGICA SINICA. DOI: 10.7541/2025.2024.0447
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EVALUATION OF IMMUNE EFFECT ON ATTENUATED STRAIN OF LARGEMOUTH BASS RANAVIRUS

  • 1.

    School of Life Sciences, Huzhou University, Huzhou 313001, China

  • 2.

    Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Key Laboratory of Fishery Environment and Aquatic Product Quality and Safety of Huzhou City, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China

  • 3.

    National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China

  • 4.

    College of Fisheries, Zhejiang Ocean University, Zhoushan 316022, China

Funds: Supported by the Zhejiang Provincial Institute Special Project (2025YSZX02); the “Vanguard Geese+X” Research and Development Breakthrough Plan in Zhejiang Province (2024C02005); National Key Research and Development Program “Innovations in Marine Agriculture and Freshwater Fisheries Technology” (2023YFD2400702)
  • Received Date: November 13, 2024
  • Rev Recd Date: January 16, 2025
  • Available Online: February 18, 2025
    Graphical Abstract
    • Abstract

  • In order to investigate the characteristics of LMBV-ZJDSS-F110, an attenuated transmissible strain of Largemouth bass virus (LMBV), this study examined various factors, including replication efficiency, virulence, in vivo viral load changes, comparative genomes, immune protection rate, expression of immune-related genes after immunization, and virulence regression of the virus. The results showed that, when cultured in FHM, the viral titer of F110 strain reached 109.1 TCID50/mL, and no mortality was observed in largemouth bass when injected at a dose of 108.0 TCID50/mL×0.1 mL. Tissue viral load tests on different generations of strains showed that the viral loads in spleen and gills of passaged strains F5, F45, and F110 were higher in the 7d than that of the 3d, whereas the viral loads of F90 did not change significantly at the two time points, which indicated that the characteristics of the transmission strain F90 had changed compared with those of F45 and F110. Comparative genomic analyses of the F5 and F110 strains showed that LMBV-ZJDSS-F110 exhibited 10 mutations, of which 7 could cause changes in the coding region. Immunization trials using both injection and immersion showed that the 108.0 TCID50/mL concentration group obtained 70% immune protection rate against the strong F5 strain. Gene expression of immune factors (TNF-α, CD8b, IgM, IgT, IFN-γ) in the head and kidney reached a peak on the 14d after immunization and was significantly higher in the injected group than that of the immersion group, with the expression of IFN-γ was significantly higher. Specifically, the expression of IFN-γ was nearly 50 times that of the control group. The results of in vivo virulence reversion test showed that F110 did not cause any mortality in 5 generations of blind in vivo transmission. The above studies indicate that LMBV-ZJDSS-F110 is a safe and effective attenuated vaccine strain, which provides a pathway for the immunization of largemouth bass virus.

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