LI Wen-Xiang, XIE Jun, SONG Rui, ZOU Hong, WU Shan-Gong, WANG Gui-Tang. EFFECTS OF pH STRESS ON CORTISOL AND NON-SPECIFIC IMMUNITY OF|CARASSIUS AURATUS GIBELIO[J]. ACTA HYDROBIOLOGICA SINICA, 2011, 35(2): 256-261. DOI: 10.3724/SP.J.1035.2011.00256
Citation: LI Wen-Xiang, XIE Jun, SONG Rui, ZOU Hong, WU Shan-Gong, WANG Gui-Tang. EFFECTS OF pH STRESS ON CORTISOL AND NON-SPECIFIC IMMUNITY OF|CARASSIUS AURATUS GIBELIO[J]. ACTA HYDROBIOLOGICA SINICA, 2011, 35(2): 256-261. DOI: 10.3724/SP.J.1035.2011.00256

EFFECTS OF pH STRESS ON CORTISOL AND NON-SPECIFIC IMMUNITY OF|CARASSIUS AURATUS GIBELIO

  • Received Date: March 18, 2010
  • Rev Recd Date: September 24, 2010
  • Published Date: March 24, 2011
  • Effects of pH stress on cortisol and non-specific immunity of Carassius auratus gibelio were investigated.Acidity and Alkalinity of the aquaculture water were maintained at pH 6.0, 7.4, 8.5 and 9.5, where pH 7.4 was used ascontrol. pH value of the aquaculture water was adjusted by HCl (1 N) or NaOH (1 N). Fish blood was sampled at 0, 14and 30 days during the experiment. Leucocytes isolated by 55% percoll were used for determination of phagocytosis andrespiratory burst activity by flow cytometry, and the plasma was used to survey the cortisol concentration and lysozymeactivity. Fish weight was measured when pH stress protocol finished. The fish was then challenged by the bacteria Flavobacteriumcolumnare, and dead fish was recorded after 7 days of the challenge. Under the stress of pH 6.0, phagocytosisof leucocytes decreased significantly at the 14 and 30 day, but no significant differences were found in respiratoryburst activity and cortisol level. There were significant differences in lysozyme activity and growth between pH 6.0 andcontrol group. No fish died from the bacteria challenge. Under pH 8.5 and 9.5 stress, the cortisol concentration increasedsignificantly, and phagocytosis and respiratory burst activity decreased significantly at the 14 and 30 day. The challengeof bacteria resulted in fish death. The results suggested that non-specific immunity and growth of the carp was heavilyaffected by the pH 8.5 and 9.5 stress, and phagocytosis and respiratory burst activity of fish leucocytes determined byflow cytometry could assess the influence of stress on fish health.
  • [1]
    Pickering A D. Environmental stress and the survival ofbrown trout, Salmo trutta [J]. Freshwater Biology, 1989, 21:47—55
    [2]
    Pickering A D, Pottinger T G. Stress responses and diseaseresistance in salmonid fish: Effects of chronic elevation ofplasma cortisol [J]. Fish Physiology and Biochemistry, 1989,7: 253—258
    [3]
    Wang W B, Wang J G, Li A H, et al. Changes of cortisol andlysozyme levels in Carassius auratus blood after crowdingstress and the fish sensitivity to pathogen [J]. Journal ofFishery Sciences of China, 2004, 11(5): 408—412 [王文博,汪建国, 李爱华, 等. 拥挤胁迫后鲫鱼血液皮质醇和溶菌酶水平的变化及对病原的敏感性.中国水产科学, 2004,11(5): 408—412]

    Wang W B, Wang J G, Li A H, et al. Changes of cortisol andlysozyme levels in Carassius auratus blood after crowdingstress and the fish sensitivity to pathogen [J]. Journal ofFishery Sciences of China, 2004, 11(5): 408—412 [王文博,汪建国, 李爱华, 等. 拥挤胁迫后鲫鱼血液皮质醇和溶菌酶水平的变化及对病原的敏感性.中国水产科学, 2004,11(5): 408—412]
    [4]
    Tomasso J R, Davis K B, Parker N C. Plasma corticosteroiddynamics in channel catfish, Ictalurus punctatus (Rafinesque),during and after oxygen depletion [J]. Journal ofFish Biology, 1981, 18: 519—526
    [5]
    Jiang L, Pan L, Fang B. Effect of dissolved oxygen on immuneparameters of the white shrimp Litopenaeus vannamei[J]. Fish & Shellfish Immunology, 2005, 18: 185—188
    [6]
    Tomasso J R, Davis K B, Simco B A. Plasma corticosteroiddynamics in channel catfish (Ictalurus punctatus) exposed toammonia nitrite [J]. Canadian Journal of Fisheries andAquatic Sciences, 1981, 38: 1106—1112
    [7]
    Kampen A H, Tollersrud T, Larsen S, et al. Repeatability offlow cytometric and classical measurement of phagocytosisand respiratory burst in bovine polymorphonuclear leucocytes[J]. Veterinary Immunology and Immunopathology,2004, 97: 105—114
    [8]
    Harford A J, O’Halloran K, Wright P F A. Flow cytometricanalysis and optimisation for measuring phagocytosis inthree Australian freshwater fish [J]. Fish & Shellfish Immunology,2006, 20: 562—573
    [9]
    Avenda?o A, Sales-Pardo I, Marin L, et al. Oxidative burstassessment and neutrophil–platelet complexes in unlysedwhole blood [J]. Journal of Immunological Methods, 2008,339: 124—131
    [10]
    Pickering A D, Pottinger T G. Seasonal and diel changes inplasma cortisol levels of the brown trout, Salmo trutta L [J].General and Comparative Endocrinology, 1983, 49: 232—239
    [11]
    White-Owen C, Alexander J W, Sramkoski R M, et al. Rapidwhole-blood microassay using flow cytometry for measuringneutrophil phagocytosis [J]. Journal of Clinical Microbiology,1992, 30: 2071—2076
    [12]
    Goedken M, Guise S D. Flow cytometry as a tool to quantifyoyster defence mechanisms [J]. Fish & Shellfish Immunology,2004, 16: 539—552
    [13]
    Ndong D, Chen Y Y, Lin Y H, et al. The immune response oftilapia Oreochromis mossambicus and its susceptibility toStreptococcus iniae under stress in low and high temperatures[J]. Fish & Shellfish Immunology, 2007, 22: 686—694
    [14]
    Strange R J, Schreck C B. Anesthetic and handling stress onsurvival and cortisol concentration in yearling Chinooksalmon (Oncorhynchus tshawytscha) [J]. Journal of theFisheries Research Board of Canada, 1978, 35: 345—349
    [15]
    Wang W B, Wang J G, Li A H, et al. Changes of cortisol andlysozyme levels in Carassius auratus blood after handlingstress [J]. Acta Hydrobiologica Sinica, 2004, 28(6): 682—684 [王文博, 汪建国, 李爱华, 等. 振荡胁迫后鲫血液皮质醇和溶菌酶水平的变化. 水生生物学报, 2004, 28(6):682—684]

    Wang W B, Wang J G, Li A H, et al. Changes of cortisol andlysozyme levels in Carassius auratus blood after handlingstress [J]. Acta Hydrobiologica Sinica, 2004, 28(6): 682—684 [王文博, 汪建国, 李爱华, 等. 振荡胁迫后鲫血液皮质醇和溶菌酶水平的变化. 水生生物学报, 2004, 28(6):682—684]
    [16]
    Pickering A D, Pottinger T G, Sumpter J P, et al. Effects ofacute and chronic stress on the levels of circulating growthhormone in the rainbow trout, Oncorhynchus mykiss [J].General and Comparative Endocrinology, 1991, 83: 86—93
    [17]
    Mock A, Peters G. Lysozyme activity in rainbow trout, Oncorhynchusmykiss (Walbaum), stressed by handling, transportand water pollution [J]. Journal of Fish Biology, 1990,37: 873—885
    [18]
    Demers N E, Bayne C J. The immediate effects of stress onhormone and plasma lysozyme in rainbow trout [J]. Developmental& Comparative Immunology, 1997, 21: 363—373
    [19]
    Fevolden S E, Red K H, Fjalestad K. A combined salt andconfinement stress enhances mortality in rainbow trout (Oncorhynchusmykiss) selected for high stress responsiveness[J]. Aquaculture, 2003, 216: 67—76
    [20]
    Verghese B, Radhakrishnan E V, Padhi A. Effect of environmentalparameters on immune response of the Indian spinylobster, Panulirus homarus (Linnaeus, 1758) [J]. Fish &Shellfish Immunology, 2007, 23: 928—936
  • Cited by

    Periodical cited type(6)

    1. 魏晓雪,田晨,冯剑丰,朱琳. 磷限制条件下单胞藻胞内磷储量差异机理研究. 环境科学研究. 2022(06): 1398-1406 .
    2. 陈敏怡,王振红,罗专溪. 不同磷源对砷胁迫下2种绿藻生长的影响. 生态毒理学报. 2020(05): 218-227 .
    3. 郐安琪,赵伟华,李青云,龙萌. 铜绿微囊藻对Cd~(2+)胁迫的生理生化响应. 人民长江. 2016(16): 20-25 .
    4. 杨宋琪,吴忠兴. 斜生栅藻在不同磷条件下氮生态幅的研究. 水生生物学报. 2014(03): 510-515 . 本站查看
    5. 陈文捷,沈宏,庞兴红,吕丽媛,谢平. 三种水华藻类在振荡条件下的磷吸收动力学及生长预测研究. 长江流域资源与环境. 2013(S1): 59-65 .
    6. 吴叶宽,袁玲,黄建国,李隆云. 青蒿素对绿藻的化感效应. 中国中药杂志. 2013(09): 1349-1354 .

    Other cited types(9)

Catalog

    Article views (2203) PDF downloads (585) Cited by(15)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return