EFFECT OF THE ACTIVE MONOMER FROM RADIX ANGELICAE PUBESCENTIS ON KILLING DACTYLOGYRUS INTERMEDIUS AND ITS ACTIVE COMPONENT IDENTIFICATION

Activated components of parasiticidal Dactylogyrus intermedius extracted from Radix angelicae pubescentis were isolated and purificated by column chromatography and biologica l tracking in this study. The activity in vitro of activated components at different concentrations were determined. Then their chemical structures were identified by TLC, UV, HPLC and GC-MS. Observations implied that the killing effect of the components on D. intermedius at different concentrations were fraction Fand K1-2-3. Fraction F could reach to 100% parasiticidal rate when the concentration was 1.6 mg/L. As for K1-2-3, concentrations of 5 mg/L could reach to the parasiticidal rate of 74.9%. According to physico- chemical property examination and spectroanalysis, fraction F and K1-2-3 were identified as the osthole and scopoletine.