ISOLATION AND IDENTIFICATION OF A CYANOPHAGE IN LAKE TAIHU

LIU Lu; GUO Zong-Lou; HUANG Pu; WANG Hao; XU Li-Hong

doi:10.3724/SP.J.1035.2012.00339

Volume 36 Issue 2

Mar. 2014

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ACTA HYDROBIOLOGICA SINICA > 2012 > 36(2): 339-343. > DOI: 10.3724/SP.J.1035.2012.00339

LIU Lu, GUO Zong-Lou, HUANG Pu, WANG Hao, XU Li-Hong. ISOLATION AND IDENTIFICATION OF A CYANOPHAGE IN LAKE TAIHU[J]. ACTA HYDROBIOLOGICA SINICA, 2012, 36(2): 339-343. DOI: 10.3724/SP.J.1035.2012.00339

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ISOLATION AND IDENTIFICATION OF A CYANOPHAGE IN LAKE TAIHU

College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China;
2. College of Medicine, Zhejiang University, Hangzhou 310058, China

Received Date: December 23, 2010
Rev Recd Date: November 02, 2011
Published Date: March 24, 2012

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Abstract

It is the first report on Cyanophage isolated from Lake Taihu and infected Microcystis aernginosa (M. aernginosa). Water samples were collected from Lake Taihu during bloom season. The water sample was filtered through 0.22 μm polycarbonate membranes and concentrated by tangential flow fitration system, and then was inoculated into exponentially growing cultures of M. aernginosa strains. A further research was done for the algae with obvious infection. The results showed that M. aernginosa 905 was lysed. The lysate was purified through the method of CsCl density gradient centrifugation and one-step growth curve for the cyanophage was also studied. When MOI was 10-5, the latent phase of cyanophage infecting M. aernginosa was 2 hours, the rise phase was 4-6 hours and stabilization was 6-12 hours. The burst size was 4 pfu/cell. The virion with an icosahedral head (nearly 50 nm in diameter) and a short tail was observed under the transmission electron microscopy. The cyanophage had not only the specificity of species but also the specificity of strains. It could only infect M. aernginosa 905. In addition, the infection activity was lost when the cyanophage was stored at -20 an℃ d -80℃ without cryoprotectants, but the infection activity maintained over 50 days at 4℃. The above findings provide a potential method for biological control of algae bloom by cyanophage.
- Cyanophage,
- M. aernginosa,
- Algae bloom in Lake Taihu

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[1]	Hu Z X, Xu N, Li A F, et al. Effects of different N:P ratios onthe growth of pseudo-nitzschia pungens, prorocentrumdonghaiense and phaeocystis globosa nonlinea [J]. Acta HydrobiologicaSinica, 2008, 32(4): 482-487 [胡章喜, 徐宁,李爱芬, 等. 氮磷比率对3 种典型赤潮藻生长的影响. 水生生物学报, 2008, 32(4): 482-487]
[2]	Zhou J, Zeng C, Wang L L. Study on characteristic of algaegrowth in Tai Lake based on nonlinear dynamic analysis [J].Acta Hydrobiologica Sinica, 2009, 33(5): 931-936 [周婕,曾诚, 王玲玲. 太湖蓝藻生长特性的非线性动力学分析.水生生物学报, 2009, 33(5): 931-936]
[3]	Kuang Q J, Ma P M, Hu Z Y, et al. Study on the evaluationand treatment of lake eutrophication by means of algae biology[J]. Journal of Safety and Environment, 2005, 5(2): 87-91 [况琪军, 马沛明, 胡征宇, 等. 湖泊富营养化的藻类生物学评价与治理研究进展. 安全与环境学报, 2005, 5(2):87-91]
[4]	Zhang Q Y, Gui J F. A kind of strategic bio-resource not to beneglected- freshwater and marine viruses and their roles inthe global ecosystem [J]. Bulletin of the Chinese Academy ofSciences, 2009, 24(4): 414-420 [张奇亚, 桂建芳. 一类不可忽视的战略生物资源--淡水与海水中的病毒及其在生态系统中的作用. 中国科学院院刊, 2009, 24(4): 414-420]
[5]	Zhang Y H, Zhao Y J, Cheng K. Quantitative relationshipamong Microcystis aernginosa, bacteriaplankton and virioplanktonin eutrophication water bodies [J]. EnvironmentalScience and Technology, 2010, 33(4): 20-23 [张一卉, 赵以军, 程凯. 富营养化水体中微囊藻、菌、病毒数量关系初步研究. 环境科学与技术, 2010, 33(4): 20-23]
[6]	Qiu T, Xu L, Cheng K, et al. The change in activity ofCyanophage PP by UV and sunlight radiation [J]. VirologicaSinica, 2004, 19(1): 58-61 [邱彤, 徐丽, 程凯, 等. 紫外光和日光对噬藻体PP 活性的影响. 中国病毒学, 2004,19(1): 58-61]
[7]	Suttle C A, Chan A M. Dynamics and distribution of chanophagesand their effect on marine Synechococcus spp [J].Applied Environmental Microbiology, 1994, 60: 3167-3174
[8]	Safferman R S, Morris M E. Algal viruses: isolation [J].Science, 1963, 140: 679-680
[9]	Adolph K W, Haselkorn R. Isolation and characterization ofa virus infecting a blue-green alga of the genus Synechococcus[J]. Virology, 1973, 54: 230-236
[10]	Adolph K W, Haselkorn R. Isolation and characterization ofa virus infecting the blue-green alga Nostoc muscorum [J].Virology, 1971, 46: 200-208
[11]	Fox J A, Booth S J, Martin E L. Cyanophage SM-2: a newblue-green algal virus [J]. Virology, 1976, 73: 557-560
[12]	Safferman R S, Diener T O, Desjardins P R, et al. Isolationand haracterization of AS-1, a phycovirus infecting theblue-green algae, Anacystis nidulans and Synechococcuscedrorum [J]. Virology, 1972, 47: 105-113
[13]	Safferman R S, Schneider I R, Steere R L, et al. PhycovirusSM-1: a virus infecting unicellular blue-green algae [J]. Virology, 1969, 37: 386-395
[14]	Gao E B, Yuan X P, Li R H, et al. Isolation of a novelcyanophage infectious to the filamentous cyanobacteriumPlanktothrix agardhii (Cyanophyceae) from Lake Donghu,China [J]. Aquatic Microbial Ecology, 2009, 54: 163-170
[15]	Takashi Y, Yukari T, Yuji T, et al. Isolation and characterizationof a cyanophage infecting the toxiccyanobacterium Microcystis aernginosa [J]. Applied andEnvironmental Microbiology, 2006, 72(2): 1239-1247
[16]	Stephen T, Peter P. Identification of cyanophage Ma-LBPand infection of the cyanobacterium Microcystis aernginosafrom an Australian Subtropical Lake by the virus [J]. Appliedand Environmental Microbiology, 2005, 71(2): 629-635
[17]	Zhao Y J, Cheng K, Shi Z L, et al. Isolation and identificationof the first cyanophage in China [J]. Progress in NatureScience, 2002, 12(9): 923-927 [赵以军, 程凯, 石正丽, 等.我国首株噬藻体(蓝藻病毒)的分离与鉴定. 自然科学进展,2002, 12(9): 923-927]
[18]	Tang X C, Song L R, Shen Y W, et al. Study of phycophagesfrom Microcystis sp in Lake Dianchi, South-west China [J].Journal of Microbiology, 2003, 23(3): 50-55 [汤显春, 宋立荣, 沈银武, 等. 滇池微囊藻病毒溶藻的研究. 微生物学杂志, 2003, 23(3): 50-55]
[19]	Yoshida T, Nagasaki K, Takashima Y, et al. Ma-LMMOIinfecting toxic Microcystis aeruginosailluminates diversecyanophage genome strategies [J]. Journal of Bacteriology,2008, 190(5): 62-72
[20]	Safferman R S. Classification and nomenclature of viruses ofcyanobacteria [J]. Intervirology, 1983, 19: 61
[21]	Sullivan M B, Waterbury J B, Chisholm S W. Cyanophagesinfecting the oceanic cyanobacterium Prochlorococcus [J].Nature, 2003, 424: 1047-1051
[22]	Yoshida T, Takashima Y, Tomaru Y, et al. Isolation andcharacterization of a cyanophage infecting the toxic cyanobacteriumMicrocystis aeruginosa [J]. Appl EnvironMicrobiol, 2006, 72: 1239-1247
[23]	Zhang Y Z, Teng H G, Lü S R, et al. Preparation of stabilizerof freeze-dried adenovirus-based live HIV vaccine [J]. ChineseJournal of Biologicals, 2007, 20(2): 104-106 [张一折,滕洪刚, 吕帅然, 等. 重组HIV-1 腺病毒载体活疫苗的冻干保护剂研究. 中国生物制品学杂志, 2007, 20(2): 104-106]

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