SCREENING AND VERIFICATION OF MICROSATELLITE MARKERS CLOSELY RELATED WITH RAPID GROWTH TRAITS IN LARGE YELLOW CROAKER LARIMICHTHYS CROCEA

To screen the molecular markers closely related with growth traits and identify the validity of these markers in large yellow croaker, a family and a population were used as material and three steps were included. The first step was makers screening. In this step, a family with 60 extreme individuals was used as genetic material. The second step was markers preliminary verification. In this step, a family with 120 random individuals was used as genetic material. The last step was markers verification once again. A population with 60 extreme individuals was used. Two microsatellite markers closely related with growth traits were obtained. LYC0088 was significantly correlated with body height, body weight (P0.01), and body length (P0.05). LYC0143 was significantly correlated with body length and body weight (P0.05), and body height (P0.01). For LYC0088 and LYC0143 loci, the advantage allele was E and A, respectively. The advantage genotypes were both AB. The advantage combination of genotype was AB/AB. These two loci presented additive effects. The results provide valuable genetic markers for conducting large yellow croaker molecular marker-assisted breeding.