THE EFFECT OF DIFFERENT INSETTING-NUCLEUS LOCATIONS ON BODY PHYSIOLOGICAL METABOLISM IN VISCERAL MASS OF HYRIOPSIS CUMINGII

To ascertain the effect of different inserting-nucleus locations in viscera mass on body damage in Hyriopsis cuming, a 50d trial was carried out on six groups. The six groups were randomly divided into one control group and five experimental groups according to the five locations in viscera mass (Ⅰ: the front end of axe foot visceral mass; Ⅱ: the central of axe foot visceral group; Ⅲ: nearly gonad department; Ⅳ: nearly stomach; Ⅴ: nearly kidney) to insert nucleus. The lymphatic blood samples were extracted respectively from closed shell muscle nucleus at 5d, 10d, 20d and 50d (thd) after inserting-nucleus to detect uric acid (UA) and physiological indexes of liver and kidney, and to clarify the influence of calcium content and alkaline phosphatase (AKP) activity about nacre deposition. Our results indicated: (1) Compared with the control group, the physiologic index of experimental group showed significant differences (P0.05). (2) The UA levels at 50thd of five inserting-nucleus groups were markedly lower than that of the other time groups, and UA of Ⅴ group was significantly higher than that of the other experimental groups during 10thd to 50thd. (3) The levels of blood urea nitrogen (BUN) and blood urine creatinine (Cr) of five experimental groups at 5thd were markedly higher than those at the 50thd (P0.05). There into, the levels of Ⅰand Ⅲ groups showed no significant difference during 20thd to 50thd, the BUN levels of Ⅴ group were significantly higher than those of the other groups during experiment period except for the 5thd (P0.05). (4) The activities of glutamicoxaloacetic transaminase (AST) and glutamate alanine aminotransferase (ALT) of the Ⅰ, Ⅱ, Ⅲ groups from 5thd to 20thd were markedly lower than that at the 50thd (P0.05), the ALT and AST activities of Ⅳ group were significantly higher than that of the other groups during experiment period except for the 10thd. The AST and ALT activities were significantly higher than that of Ⅰ, Ⅱ, Ⅲ groups and were the highest after Ⅳ group from 20thd to 50thd. (5) Blood calcium content (Ga) ofⅠgroup at 10thd was significantly lower than that at 5thd, and no significant difference after 10thd. In Ⅱ, Ⅲ groups, the Ga increased firstly and then decreased, which reached to the peak at the 20thd. The Ga of Ⅳ group had a decreasing trend with experimental period, the level of Ⅴ group was significantly higher than that of the other four groups at 1050thd (P0.05). AKP activities showed no significant difference in Ⅱ, Ⅲ groups, but decreased significantly inⅠ and Ⅳ groups (P0.05). The AKP activity of Ⅴ group reached the peak at 20thd. The present study showed the body physiological index was significantly changed before 20d of inserting-nucleus in visceral mass of Hyriopsis cuming, and then the value kept stable. This result indicated that the 20 days may be a critical period for repair of body damage, kidney, and liver function recovery, and the gradual stabilization of the calcium content. Our study provided theory basis for fresh-water pearl cultivation in the visceral mass of Hyriopsis cumingii.