CLONING,SPATIOTEMPORAL EXPRESSION AND SNPS IDENTIFICATION OF HDAC1 GENE IN HARD CLAM MERETRIX MERETRIX

Mm-HDAC1 cDNA was cloned by SMART RACE techniques using 454 cDNA library of Meretrix meretrix. The bioinformatics and expression profiles in different tissues and developmental stages were analyzed, and SNPs were screened. The results indicated that the full length cDNA of Mm-HDAC1 gene was 3065 bp, containing a complete 1599 bp ORF that encodes 532 amino acids. Comparison of animal acid sequence, M. meretrix shared 74.3%78.5% identity with others, suggesting that Mm-HDAC1 was highly conservative. The result of qRT-PCR showed that Mm-HDAC1 expressed in all six tissues, and its expression was significantly higher in mantle than other tissues (P0.01). Moreover, the expression of Mm-HDAC1 gradually increased with the process of the development with the highest level in umbo larvae stage (P0.01). 19 SNPs in exons of Mm-HDAC1 were identified and 3 SNPs were potentially associated with M. meretrix growth (627AT, 924TC and 1266TC).