MOLECULAR CLONING AND GENETIC ONTOGENY OF CCT DURING THE DEVELOPMENT OF LARGE YELLOW CROAKER LARVAE (LARIMICHTHYS CROCEA)

In the present study, the full length of CCT (CTP: choline phosphate cytidylyltranferase) cDNA from large yellow croaker (Larimichthys crocea) was obtained by homology-based cloning and rapid amplification of cDNA ends (RACE) techniques. Sequence analysis showed that the full length of CCT cDNA (GenBank accession No. KF006240.1) was 2419 bp, which consisted of 1107 bp open-reading frame (ORF) encoding 369 amino acids, a 273 bp 5'-untranslated region (5'UTR) and a 1010 bp 3'-untranslated region (3'UTR). Phylogenetic tree analysis showed that the CCT gene of large yellow croaker had a closer relationship with Takifugu rubripes than others. The expression of CCT increased significantly at first, and then decreased, and peaked at 15 day after hatching. The genetic ontogeny of CCT was related to the development of large yellow croaker digestive system.