EFFECTS OF DIETARY LIPID LEVELS ON TOR PATHWAY IN JUVENILE TURBOT (SCOPHTHALMUS MAXIMUS L.)

This study investigated the effects of dietary lipid levels on the TOR pathway in juvenile turbot using diets containing graded levels of lipid 11.69% (L11.69) and 16.58% (L16.58) (dry weight) to feed triple groups of juvenile turbot (8.6±0.01 g) for a 97d trial. The lipids level in diet did not impact survival rate and SGR. Compared those in L11.69 group, L16.58 group increased the gene expression of TOR, 4EBP1 but decreased 4EBP2 in liver, and it increased the phosphorylation of pAKT-S473, pAKT-T308, pmTOR-S2448, and p4EBP1-T37/46. In the muscle, L16.58 group had decreased TOR and 4EBP1 associated with pAKT-S473, pmTOR-S2448 and p4EBP1-T37/46 compared with those in L11.69 group. In conclusion, L16.58 diet can activate TOR pathway in liver while partially suppress TOR pathway in muscle of junvenile turbot compare with L11.69 diet.