THE PRELIMINARY STUDY ON CLONING, EXPRESSION PROFILES AND FUNCTION OF GRASS CARP (CTENOPHARYNGODON IDELLUS) TRIM32

The TRIM family, an important member in natural immune system, regulates cell proliferation, differentiation, apoptosis, tumor suppression, antiviral activity and other processes. The TRIM32 full-length sequence of grass carp (Ctenopharyngodon idellus) was cloned by using PCR method. It was 1980 bp in length encoding 660 amino acids. The TRIM32 protein has three typical structural features of the TRIM family. Sequence analysis indicated that TRIM32 of grass carp shared the highest identity with zebrafish, which belongs to the same branch on the phylogenetic trees. Immunofluorescence and Western-blotting showed that TRIM32 successfully expressed in cytoplasmic speckles of EPC cells. Tssue distribution analysis showed that TRIM32 was widely expressed, especially in the renal tissue. TRIM32 was significantly higher in zygote period, cleavage and blastula stage than that of the other stages during different stages of embryonic development, and then decreased until hatching in which it increased significantly. In addition, grass carp TRIM32 can activate the nuclear factor NF-κB based on dual luciferase assay and induce inflammation.