EFFECTS OF REOVIRUS INFECTION ON THE INTESTINAL MICROBIOTA DIVERSITY OF GRASS CARP (<i>CTENOPHARYNGODON IDELLA</i>)

ZHU Wen-Gen; LI Xing-Hao; RAO Liu-Yu; HUANG Jie; YU Yu-He; XIAO Fan-Shu; YAN Qing-Yun

doi:10.7541/2019.014

Volume 43 Issue 1

Dec. 2018

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ACTA HYDROBIOLOGICA SINICA > 2019 > 43(1): 109-116. > DOI: 10.7541/2019.014

ZHU Wen-Gen, LI Xing-Hao, RAO Liu-Yu, HUANG Jie, YU Yu-He, XIAO Fan-Shu, YAN Qing-Yun. EFFECTS OF REOVIRUS INFECTION ON THE INTESTINAL MICROBIOTA DIVERSITY OF GRASS CARP (CTENOPHARYNGODON IDELLA)[J]. ACTA HYDROBIOLOGICA SINICA, 2019, 43(1): 109-116. DOI: 10.7541/2019.014

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EFFECTS OF REOVIRUS INFECTION ON THE INTESTINAL MICROBIOTA DIVERSITY OF GRASS CARP (CTENOPHARYNGODON IDELLA)

1.
Key Laboratory of Biodiversity and Conservation of Aquatic Organisms, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China
2.
University of Chinese Academy of Sciences, Beijing 100049, China
3.
Environmental Microbiomics Research Center, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510006, China

Funds: Supported by the National Natural Science Foundation of China (31672262, 31400109)

Received Date: February 25, 2018
Rev Recd Date: April 16, 2018
Available Online: September 16, 2018
Published Date: December 31, 2018

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Abstract

Abstract

The grass carp (Ctenopharyngodon idellus) infected with the hemorrhage virus was used to analyze the intestinal microbiota by using high-throughput sequencing. The results showed that microbiota significantly differed between grass carp reovirus (GCRV)-infected and control grass carp (multiple response permutation procedure (MRPP), analysis of similarity (Anosim), and Adonis; P<0.01). When we compared alpha diversity, we found that Shannon-Wiener index, inverse of Simpson's original index and Pielou’s evenness index of intestinal microbiota were all significantly decreased after infecting with GCRV (independent samplest-test, P<0.05). Besides, we found that the individual difference within GCRV-infected group was significantly greater than those in the controls (Wilcoxon signed-rank test,P<0.05), suggesting that the intestinal microbiota in GCRV-infected grass carps became disorganized and lost their original balance. Proteobacteria, Firmicutes, Fusobacteriaceae, and Bacteroidetes were dominant phylum in both GCRV-infected group and controls. However, the relative abundance of operational taxonomic units (OTUs) between the two groups was different. For example, OTU_69 (Pasteurellaceae), OTU_504 (Comamonadaceae), and OTU_1898 (Cetobacterium) were all significantly lower than that of controls (t-test, P<0.05), confirming that GCRV could make intestinal microbiota of grass carps disorganized. The stability of intestinal microbiota is important for health of host. By detecting changes in the intestinal microbiota of GCRV-infected grass carp, we will be able to provide theoretical basis and data reference for the prevention and treatment of common disease of farmed fish, as well as providing a reference for healthy breeding.
- Grass carp reovirus,
- Intestinal microbiota,
- 16S rRNA gene,
- High-throughput sequencing,
- Grass carp

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EFFECTS OF REOVIRUS INFECTION ON THE INTESTINAL MICROBIOTA DIVERSITY OF GRASS CARP (CTENOPHARYNGODON IDELLA)

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