PARENTAGE ANALYSIS OF COREIUS GUICHENOTI USING MICROSATELLITES

To quickly and effectively distinguish different families and pedigrees of Coreius guichenoti, the present study screened twenty microsatellite loci with high polymorphism and stable amplification from the reported forty microsatellite loci using 339 individuals from eight families of C. guichenoti. Through this study, a paternity technology combined with fluorescent microsatellites and multiple capillary electrophoresis in C. guichenoti were established. Genetic diversity analysis revealed that the average allele number (N_a) of eight families of C. guichenoti, the average polymorphism information content (PIC), the average expected heterozygosity (H_e), and the average observed heterozygosity (H_o) were 9, 0.616, 0.659, and 0.691, respectively. Genetic diversity indices of the offspring group were significantly lower than those of the parent group. Parentage analysis revealed that the combined exclusion probability of a first candidate parent given only the genotype of the offspring (CE-1P), a second candidate parent given the genotype of the offspring and one known parent (CE-2P) and a candidate parent poor give only the genotype of the offspring (CE-PP) were 0.99954473, 0.99999825 and 1.00000000, respectively, indicating that the accurate rate of parentage assignment was 100%. Thus we established a reliable paternity technology of C. guichenoti, which can provide scientific basis for its genealogical management, population genetic management, and assessment of stock enhancement effect.