DEVELOPMENT OF EST-SSR MARKERS AND ANALYSIS OF GROWTH TRAIT IN SILVER CARP

Silver carp is one of important breeding fish in China. It is necessary to study the growth traits markers to accelerate the molecular marker-assisted breeding techniques. The simple sequence repeat (SSR) from the transcriptome of egg in silver carp were characterized by high-throughput Illumina sequencing, and genotypes associated growth traits were analyzed. The main results are as follows: (1) 77129 unigenes were detected by assembly, 31.7% of 24458 ESTs contained repeat motifs of various types (dinucleotide, trinucleotide, tetranucleotide and pentanucleotide) and lengths with AC being the most abundant dinucleotides one. (2) 175 EST-SSR primers were randomly characterized and 36 polymorphic EST-SSR were developed in silver carp. The results of Blast indicated that 10 EST-SSR genes are known. (3) The results showed that two loci were significantly correlated with growth traits in full-sibling family in silver carp. One locus SCE26 was significantly (P<0.05) correlated with body length and body weight, and SCE65 was significantly (P<0.05) correlated with condition factor. This study will provide theoretical basis and technical methods for the development of the marker-assisted breeding system in silver carp.