Volume 47 Issue 2
Jan.  2023
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LIU Er-Tian, CHEN Yi, ZHAN Xin, GU Zhi-Feng, WANG Ai-Min. CORRELATION BETWEEN FIVE MICROSATELLITE MARKERS AND FOUR GROWTH TRAITS IN PTERIA PENGUIN[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 332-338. DOI: 10.7541/2022.2021.0214
Citation: LIU Er-Tian, CHEN Yi, ZHAN Xin, GU Zhi-Feng, WANG Ai-Min. CORRELATION BETWEEN FIVE MICROSATELLITE MARKERS AND FOUR GROWTH TRAITS IN PTERIA PENGUIN[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 332-338. DOI: 10.7541/2022.2021.0214
shu

CORRELATION BETWEEN FIVE MICROSATELLITE MARKERS AND FOUR GROWTH TRAITS IN PTERIA PENGUIN

  • 1.

    Ocean College, Hainan University, Haikou 570228, China

  • 2.

    State Key Laboratory of Marine Resource Utlization in South China Sea, Hainan University, Haikou 570228, China

Funds: Supported by the National Natural Science Foundation of China (31772847)
  • Received Date: August 25, 2021
  • Rev Recd Date: September 21, 2021
  • Available Online: July 07, 2022
  • Published Date: February 14, 2023
    Graphical Abstract
    • Abstract
  • The winged pearl oyster, Pteria penguin, is a widely distributed large pearl oyster species, which can produce large-sized mabé (half-pearl), and exhibit strong tolerance to severe environment. However, genetic improvement of P. penguin was still in its infancy. In this study, the correlation between five microsatellite loci and four growth traits (shell height, shell length, shell width and total weight) of 500 winged pearl oysters were analyzed by One-way ANOVA. Multiple comparisons between different genotypes of microsatellite markers and growth traits were performed. It was shown that the 5 loci in this study are all highly polymorphic loci, among which, QEB-D15 and CL-232 were extremely significantly associated with the shell width of the winged pearl oyster (P<0.01). The individuals with genotype 239/263 and 239/273 at QEB-D15 had the maximum and the minimum shell width, respectively. The 263 bp allele might positively correlate with the shell width, while the 273 bp allele might negatively correlate with the shell width. The average values of shell length, shell width and total weight of individuals with genotype 157/174 at locus CL-232 were larger than that of other genotypes, suggested that this genotype was the dominant genotype. Individuals with genotype of 177/192 at locus CL-232 had the smallest shell length, shell width, shell height and total weight, indicated that the 177/192 may be an inferior genotype. In addition, the shell width is correlated with two SSR loci, which indicates that the shell width is a phenotypic trait that may not be controlled by a single gene. This study could provide valuable information for molecular marker-assisted selection of P. penguin.
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  • [1]
    黄桂菊, 喻达辉, 郭奕惠, 等. 珍珠贝基于16S rRNA基因序列的亲缘关系研究 [J]. 南方水产, 2009, 5(6): 47-53.

    Huang G J, Yu D H, Guo Y H, et al. Phylogenetic relationship of pearl oysters in genus Pinctada based on partial mitochondrial 16S rRNA sequence [J]. South China Fisheries Science, 2009, 5(6): 47-53.
    [2]
    徐宗芹, 王梅芳, 梁飞龙, 等. 企鹅珍珠贝生殖细胞的发生 [J]. 广东海洋大学学报, 2009, 29(6): 32-35. doi: 10.3969/j.issn.1673-9159.2009.06.007

    Xu Z Q, Wang M F, Liang F L, et al. Gametogenesis in Pteria penguin (Röding) (Mollusca, Bvalvia) [J]. Journal of Guangdong Ocean University, 2009, 29(6): 32-35. doi: 10.3969/j.issn.1673-9159.2009.06.007
    [3]
    Li H M, Liu B S, Huang G J, et al. Characterization of transcriptome and identification of biomineralization genes in winged pearl oyster (Pteria penguin) mantle tissue [J]. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2017(21): 67-76. doi: 10.1016/j.cbd.2016.12.002
    [4]
    王梅芳, 余祥勇, 刘永, 等. 马氏珠母贝杂交与自繁后代及企鹅珍珠贝间同工酶差异分析 [J]. 农业生物技术学报, 2005, 13(6): 777-782. doi: 10.3969/j.issn.1674-7968.2005.06.018

    Wang M F, Yu X Y, Liu Y, et al. Genetic analysis of isozyme in both Pteria penguin and Pinctada martensii self-reproducing and hybridizing stock offspring [J]. Journal of Agricultural Biotechnology, 2005, 13(6): 777-782. doi: 10.3969/j.issn.1674-7968.2005.06.018
    [5]
    顾志峰, 王清印, 方建光, 等. 企鹅珍珠贝(Pteria penguin)在海南黎安港的生长特征 [J]. 海洋与湖沼, 2009, 40(4): 423-429. doi: 10.3321/j.issn:0029-814X.2009.04.006

    Gu Z F, Wang Q Y, Fang J G, et al. Growth of cultured winged pearl oyster (Pteria penguin) in li’an lagoon, Hainan [J]. Oceanologia et Limnologia Sinica, 2009, 40(4): 423-429. doi: 10.3321/j.issn:0029-814X.2009.04.006
    [6]
    郭奕惠, 范嗣刚, 黄桂菊, 等. 企鹅珍珠贝研究现状与展望 [J]. 江苏农业科学, 2014, 42(2): 195-198. doi: 10.3969/j.issn.1002-1302.2014.02.068

    Guo Y H, Fan S G, Huang G J, et al. Research status and prospect of Pteria penguin [J]. Jiangsu Agricultural Sciences, 2014, 42(2): 195-198. doi: 10.3969/j.issn.1002-1302.2014.02.068
    [7]
    余祥勇, 王梅芳, 叶富良. 企鹅珍珠贝个体发生及人工育苗的研究 [J]. 海南大学学报(自然科学版), 2000, 18(3): 266-269. doi: 10.15886/j.cnki.hdxbzkb.2000.03.012

    Yu X Y, Wang M F, Ye F L. Development and artificial propagation of Pteria penguin [J]. Natural Science Journal of Hainan University, 2000, 18(3): 266-269. doi: 10.15886/j.cnki.hdxbzkb.2000.03.012
    [8]
    张国范, 刘晓. 关于贝类遗传改良几个问题的讨论 [J]. 水产学报, 2006, 30(1): 130-137.

    Zhang G F, Liu X. Theory and method of genetic improvement in mariculture mollusks: a review [J]. Journal of Fisheries of China, 2006, 30(1): 130-137.
    [9]
    李俊辉, 罗燕秋, 钟雅诗, 等. 近交对马氏珠母贝生长性状、遗传多样性及矿化基因表达的影响 [J]. 南方农业学报, 2017, 48(1): 132-138. doi: 10.3969/j:issn.2095-1191.2017.01.132

    Li J H, Luo Q Y, Zhong Y S, et al. Effects of inbreeding on growth traits, genetic diversity and biomineraliazation gene expression of Pinctada fuctada martensii [J]. Journal of Southern Agriculture, 2017, 48(1): 132-138. doi: 10.3969/j:issn.2095-1191.2017.01.132
    [10]
    李思发, 杨怀宇, 邹曙明. 快速近交对团头鲂遗传结构的影响和近交效应的估算 [J]. 水产学报, 2005, 29(2): 161-165.

    Li S F, Yang H Y, Zou S M. Effects on genetic structure from quick inbreeding and estimation of inbreeding response in cultured populations of Megalobrama amblycephala [J]. Journal of Fisheries of China, 2005, 29(2): 161-165.
    [11]
    刘宇飞, 白俊杰, 李凯彬, 等. 剑尾鱼RW-H近交系20个微卫星位点的遗传结构 [J]. 中国水产科学, 2007, 14(4): 602-607. doi: 10.3321/j.issn:1005-8737.2007.04.011

    Liu Y F, Bai J J, Li K B, et al. Genetic structure of 20 microsatellite loci in RW-H swordtail fish (Xiphophorus helleri) inbred strain [J]. Journal of Fishery Sciences of China, 2007, 14(4): 602-607. doi: 10.3321/j.issn:1005-8737.2007.04.011
    [12]
    傅强. 虾夷扇贝(Patinopecten yessoensis)近交衰退的生物学效应及遗传机理研究 [D]. 青岛: 中国海洋大学, 2014: 66-69.

    Fu Q. Biological effects and genetic mechanism of inbreeding depression in Japanese scallop (Patinopecten yessoensis) [D]. Qingdao: Ocean University of China, 2014: 66-69.
    [13]
    Ji Y J, Zhang D X, Hewitt G M, et al. Polymorphic microsatellite loci for the cotton bollworm Helicoverpa armigera (Lepidoptera: Noctuidae) and some remarks on their isolation [J]. Molecular Ecology Notes, 2003, 3(1): 102-104. doi: 10.1046/j.1471-8286.2003.00366.x
    [14]
    Keong B P, Siraj S S, Daud S K, et al. Identification of quantitative trait locus (QTL) linked to dorsal fin length from preliminary linkage map of molly fish, Poecilia sp [J]. Gene, 2014, 536(1): 114-117. doi: 10.1016/j.gene.2013.11.068
    [15]
    Guo S, Zou G, Yang G. Development of microsatellite DNA markers of grass carp (Ctenopharyngodon idella) and their cross-species application in black carp (Mylopharyngodon piceus) [J]. Conservation Genetics, 2008, 10(5): 1515-1519.
    [16]
    王解香, 于凌云, 白俊杰, 等. 草鱼EST-SSR标记及5个不同地域群体的遗传结构分析 [J]. 动物学杂志, 2011, 46(5): 24-32.

    Wang J X, Yu L Y, Bai J J, et al. Development of EST-SSR markers and analysis of genetic diversity in five populations of grass carp (Ctenopharyngodon idella) [J]. Chinese Journal of Zoology, 2011, 46(5): 24-32.
    [17]
    李晶晶. 两种双壳贝类和光棘球海胆微卫星标记的分离及特性研究 [D]. 青岛: 中国海洋大学, 2008: 23-28.

    Li J J. Isolation and characterization of microsatellite markers in two species of bivalve and sea urchin Strongylocentrotus nudus [D]. Qingdao: Ocean University of China, 2008: 23-28.
    [18]
    Pérez F, Ortiz J, Zhinaula M, et al. Development of EST-SSR markers by data mining in three species of shrimp: Litopenaeus vannamei, Litopenaeus stylirostris, and Trachypenaeus birdy [J]. Marine Biotechnology (New York, N Y) , 2005, 7(5): 554-569. doi: 10.1007/s10126-004-5099-1
    [19]
    童馨. 浅色黄姑鱼和凡纳滨对虾养殖群体的遗传多样性分析 [D]. 武汉: 华中农业大学, 2007: 23-32.

    Tong X. Genetic diversity of cultured stocks of Chu’s croaker (Nibea coibor) and Pacific white shrimp (Litopenaeus vannamei) [D]. Wuhan: Huazhong Agricultural University, 2007: 23-32.
    [20]
    李云峰, 刘卫东, 高祥刚, 等. 虾夷扇贝外套膜和肾脏组织cDNA文库构建以及EST的初步分析 [J]. 中国水产科学, 2010, 17(3): 578-585.

    Li Y F, Liu W D, Gao X G, et al. Construction of cDNA libraries from mantle and kidney of Japanese scallop (Mizuhopecten yessoensis) and ESTs analysis [J]. Journal of Fishery Sciences of China, 2010, 17(3): 578-585.
    [21]
    李红蕾, 宋林生, 王玲玲, 等. 栉孔扇贝EST中微卫星标记的筛选 [J]. 高技术通讯, 2003, 13(12): 72-75. doi: 10.3321/j.issn:1002-0470.2003.12.015

    Li H L, Song L S, Wang L L, et al. Screening microsatellite markers from EST sequence of Chlamys farreri [J]. High Technology Letters, 2003, 13(12): 72-75. doi: 10.3321/j.issn:1002-0470.2003.12.015
    [22]
    陆虎. 马氏珠母贝微卫星标记的开发以及遗传连锁图谱的构建 [D]. 海口: 海南大学, 2013: 37-45.

    Lu H. Development of microsatellite mark and construction linkage map for Pinctada martensii (Dunker) [D]. Haikou: Hainan University, 2013: 37-45.
    [23]
    王学颖, 高远镇, 杜晓东, 等. 马氏珠母贝金黄壳色系 F3 和基础群体遗传结构比较 [J]. 海洋通报, 2012, 31(3): 324-328. doi: 10.3969/j.issn.1001-6392.2012.03.014

    Wang X Y, Gao Y Z, Du X D, et al. Genetic structure of the third generation yellow colored line and base stock of pearl oyster Pinctada martensii as revealed by SSR marker system [J]. Marine Science Bulletin, 2012, 31(3): 324-328. doi: 10.3969/j.issn.1001-6392.2012.03.014
    [24]
    许友卿, 肖群平, 陈秀荔, 等. 企鹅珍珠贝微卫星分子标记的筛选 [J]. 水生态学杂志, 2011, 32(3): 88-93.

    Xu Y Q, Xiao Q P, Chen X L, et al. Screening microsatellite primer sequences Pteria penguin [J]. Journal of Hydroecology, 2011, 32(3): 88-93.
    [25]
    张群英, 黄鹤忠, 袁文成, 等. 中华鳖(Pelodiscus sinensis)EST-SSR标记与生长性状相关性分析 [J]. 海洋与湖沼, 2013, 44(6): 1654-1660. doi: 10.11693/hyhz201306036

    Zhang Q Y, Huang H Z, Yuan W C, et al. Correlation of EST-SSR markers with growth traits in Chinese soft-shelled turtles (Pelodiscus sinensis) [J]. Oceanologia et Limnologia Sinica, 2013, 44(6): 1654-1660. doi: 10.11693/hyhz201306036
    [26]
    安泉泉, 刘海金, 王桂兴, 等. 牙鲆骨骼生长性状与微卫星标记的相关性分析 [J]. 水产学报, 2012, 36(5): 641-646. doi: 10.3724/SP.J.1231.2012.27788

    An Q Q, Liu H J, Wang G X, et al. Correlation analysis of microsatellite DNA markers with skeletal growth traits of Japanese flounder (Paralichthys olivaceus) [J]. Journal of Fisheries of China, 2012, 36(5): 641-646. doi: 10.3724/SP.J.1231.2012.27788
    [27]
    陈琼. 马氏珠母贝表型性状的遗传效应以及与微卫星标记的相关性 [D]. 海口: 海南大学, 2015: 24-30.

    Chen Q. Hereditary effects of phenotypic characters and correlation with microsatellite markers of the pearl oyster, Pinctada fucata martensii [D]. Haikou: Hainan University, 2015: 24-30.
    [28]
    林先鑫, 邓正华, 陈明强, 等. 两种贝龄企鹅珍珠贝形态性状对湿重的通径分析 [J]. 南方农业学报, 2019, 50(9): 2088-2094.

    Lin X X, Deng Z H, Chen M Q, et al. Path analysis of morphological traits to wet weight of Pteria penguin at different ages [J]. Journal of Southern Agriculture, 2019, 50(9): 2088-2094.
    [29]
    魏海军, 邓正华, 陈明强, 等. 棕带仙女蛤数量性状的相关与通径分析 [J]. 南方水产科学, 2019, 15(6): 34-40. doi: 10.12131/20190126

    Wei H J, Deng Z H, Chen M Q, et al. Correlation and path analysis of quantitative traits of clam (Callista erycina) [J]. South China Fisheries Science, 2019, 15(6): 34-40. doi: 10.12131/20190126
    [30]
    Weir B S, Cockerham C C. Estimating f-statistics for the analysis of population structure [J]. Evolution, 1984, 38(6): 1358-1370.
    [31]
    Botstein D, White R L, Skolnick M, et al. Construction of a genetic linkage map in man using restriction fragment length polymorphisms [J]. American Journal of Human Genetics, 1980, 32(3): 314-331.
    [32]
    Yeh F C, Boyle T. Population genetic analysis of co-dominant and dominant markers and quantitative traits [J]. Belgian Journal of Botany, 1997(129): 157-163.
    [33]
    李琪. 皱纹盘鲍微卫星研究进展 [J]. 中国海洋大学学报(自然科学版), 2004, 34(3): 365-370.

    Li Q. Advances in studies on microsatellite markers in the Pacific abalone [J]. Periodical of Ocean University of China, 2004, 34(3): 365-370.
    [34]
    叶莹莹, 徐梅英, 郭宝英, 等. 厚壳贻贝(Mytilus coruscus) 4个群体遗传多样性的ISSR分析 [J]. 海洋与湖沼, 2012, 43(1): 113-119. doi: 10.11693/hyhz201201019019

    Ye Y Y, Xu M Y, Guo B Y, et al. ISSR analysis on genetic structure of four Mytilus coruscus populations [J]. Oceanologia et Limnologia Sinica, 2012, 43(1): 113-119. doi: 10.11693/hyhz201201019019
    [35]
    Kang J H, Kim Y K, Park J Y, et al. Development of microsatellite markers for a hard-shelled mussel, Mytilus coruscus, and cross-species transfer [J]. Genetics and Molecular Research, 2013, 12(3): 4009-4017. doi: 10.4238/2013.September.27.2
    [36]
    张曼, 陈蒙, 姫南京. 虾夷扇贝家系群体遗传结构及其微卫星标记与经济性状相关性的分析研究 [J]. 中国农学通报, 2012, 28(20): 125-130. doi: 10.11924/j.issn.1000-6850.2012-0242

    Zhang M, Chen M, Zhen N J. Genetic structure of Japanese scallop population (Patinopecten yessoensis) and correlation of microsatellite DNA markers with growth traits [J]. Chinese Agricultural Science Bulletin, 2012, 28(20): 125-130. doi: 10.11924/j.issn.1000-6850.2012-0242
    [37]
    Moen T, Agresti J J, Cnaani A, et al. A genome scan of a four-way tilapia cross supports the existence of a quantitative trait locus for cold tolerance on linkage group 23 [J]. Aquaculture Research, 2004, 35(9): 893-904. doi: 10.1111/j.1365-2109.2004.01082.x
    [38]
    郑先虎, 匡友谊, 鲁翠云, 等. 镜鲤体长、体高、体厚性状QTL定位分析 [J]. 遗传, 2011, 33(12): 1366-1373.

    Zheng X H, Kuang Y Y, Lu C Y, et al. Quantitative trait locus analysis of standard length, body depth and body thickness in mirror carp (Cyprinus carpio L.) [J]. Hereditas, 2011, 33(12): 1366-1373.
    [39]
    李仕贵, 马玉清, 何平, 等. 不同环境条件下水稻生育期和株高的QTL分析 [J]. 作物学报, 2002, 28(4): 546-550. doi: 10.3321/j.issn:0496-3490.2002.04.021

    Li S G, Ma Y Q, He P, et al. Comparative mapping of quantitative trait loci for heading date and plant height in cultivated rice (Oryza sativa L.) across environments [J]. Acta Agronomica Sinica, 2002, 28(4): 546-550. doi: 10.3321/j.issn:0496-3490.2002.04.021
    [40]
    吴滟, 付春鹏, 蒋速飞, 等. 中华绒螯蟹微卫星标记与生长性状相关性的初步分析 [J]. 水生生物学报, 2011, 35(2): 197-202. doi: 10.3724/SP.J.1035.2011.00197

    Wu Y, Fu C P, Jiang S F, et al. Preliminary studies on the correlation between microsatellite markers and growth traits in Chinese mitten crab (Eriocheir sinensis) [J]. Acta Hydrobiologica Sinica, 2011, 35(2): 197-202. doi: 10.3724/SP.J.1035.2011.00197
    [41]
    刘福平, 白俊杰, 宋红梅, 等. 尼罗罗非鱼微卫星标记与主要生长性状的相关性分析 [J]. 水产学报, 2010, 34(2): 169-177. doi: 10.3724/SP.J.1231.2010.06515

    Liu F P, Bai J J, Song H M, et al. Correlation analysis of microsatellite DNA markers with major growth traits of tilapia (Oreochromis niloticus) [J]. Journal of Fisheries of China, 2010, 34(2): 169-177. doi: 10.3724/SP.J.1231.2010.06515
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