METHANOTROPH (<i>METHYLOCOCCUS CAPSULATUS</i>, BATH) BACTERIA MEAL REPLACEMENT OF FISHMEAL ON GROWTH, LIPID DEPOSITION AND ANTIOXIDANT CAPACITY OF <i>TRACHINOTUS OVATUS</i>

GAO Wen-Hao; PENG Shu-Feng; LIAO Chun-Yan; DONG Xiao-Hui; TAN Bei-Ping; WANG Jia; CHI Shu-Yan

doi:10.7541/2023.2022.0245

Volume 47 Issue 2

Jan. 2023

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ACTA HYDROBIOLOGICA SINICA > 2023 > 47(2): 195-203. > DOI: 10.7541/2023.2022.0245 CSTR: 32229.14.SSSWXB.2022.0245

GAO Wen-Hao, PENG Shu-Feng, LIAO Chun-Yan, DONG Xiao-Hui, TAN Bei-Ping, WANG Jia, CHI Shu-Yan. METHANOTROPH (METHYLOCOCCUS CAPSULATUS, BATH) BACTERIA MEAL REPLACEMENT OF FISHMEAL ON GROWTH, LIPID DEPOSITION AND ANTIOXIDANT CAPACITY OF TRACHINOTUS OVATUS[J]. ACTA HYDROBIOLOGICA SINICA, 2023, 47(2): 195-203. DOI: 10.7541/2023.2022.0245

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METHANOTROPH (METHYLOCOCCUS CAPSULATUS, BATH) BACTERIA MEAL REPLACEMENT OF FISHMEAL ON GROWTH, LIPID DEPOSITION AND ANTIOXIDANT CAPACITY OF TRACHINOTUS OVATUS

1.
Laboratory of Aquatic Economic Animal Nutrition and Feed, College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China
2.
Golden Pompano Branch of China Fisheries Association, Zhanjiang 524088, China
3.
Calysta (Shanghai) Business Information Consulting Co., Ltd, Shanghai 200060, China

Funds: Supported by the National Key R&D Program of China (2019YFD0900200)

Received Date: June 09, 2022
Rev Recd Date: August 28, 2022
Available Online: September 20, 2022
Published Date: February 14, 2023

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Abstract

Abstract

The effects of methanotroph (Methylococcus capsulatus, Barh) bacteria meal (MBM, FeedKind^®) replaces fishmeal (FM) on the growth performance and antioxidant capacity of Trachinotus ovatus (initial mean weight 34.88±1.13 g) were conducted through a 56-day breeding trial. MBM at 0, 3%, 7% and 10.5% were added to replace fishmeal at 0, 10%, 20% and 30%, to formulate four isonitrogenous and isolipidic feeds named MBM0, MBM10, MBM20 and MBM30, respectively. The results of the experiment showed that there was no significant difference in the final mean weight, weight gain rate, specific growth rate, survival rate, hepatosomatic index, visceral index and feed factor between the groups as the MBM content in the diets increased (P>0.05). The condition factor of the MBM30 group was significantly higher than that of the MBM0 group (P<0.05). The crude protein content of whole fish in each group was not significantly different (P>0.05), while the crude fat content of whole fish in the MBM20 group was significantly higher than that in the MBM0 and MBM10 groups (P<0.05). The crude fat content of dorsal muscle in the MBM20 and MBM30 groups was significantly higher than that in the MBM0 group (P<0.05), and there was a significant positive correlation between the crude fat content in dorsal muscle and the proportion of fishmeal replaced by MBM (P<0.05). Serum triglycerides were significantly lower in the MBM20 and MBM30 groups than those in MBM0 group (P<0.05), serum glutathione transaminase activity was significantly higher in the MBM20 group than that in the MBM0 group (P<0.05), and serum albumin was significantly higher in the MBM10 group than those in MBM0 group (P<0.05). Total serum antioxidant capacity in MBM20 group was not significantly different with the MBM0 group (P> 0.05), while significantly higher than that in the MBM10 and MBM30 groups (P<0.05). Intestinal superoxide dismutase activity in the MBM20 group was significantly higher than that in the rest of the groups (P<0.05). Vacuolar area in liver through H&E-stained was significantly higher in the MBM20 and MBM30 groups than that in the MBM0 group (P<0.05). In conclusion, at 35% fishmeal content in the basal diet, MBM could replace 20%—30% of fishmeal without negatively affecting growth performance, morphological indices, body composition and serum biochemical indices of Trachinotus ovatus.

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METHANOTROPH (METHYLOCOCCUS CAPSULATUS, BATH) BACTERIA MEAL REPLACEMENT OF FISHMEAL ON GROWTH, LIPID DEPOSITION AND ANTIOXIDANT CAPACITY OF TRACHINOTUS OVATUS

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