| Citation: |
ZHANG Rui, WU Li-Yun, CAO Kun-Kun, JIN Jun-Yan, YANG YUn-Xia, ZHANG Zhi-Min, HAN Dong, LIU Hao-Kun, ZHU Xiao-Ming, Xie Shou-Qi. SODIUM BICARBONATE ON GROWTH PERFORMANCE, BLOOD BIOCHEMICAL PARAMERERS, AND INTESTINAL DAMAGE OF LARGEMOUTH BASS (MICROPTERUS SALMOIDES) UNDER CHRONIC HEAT STRESS[J]. ACTA HYDROBIOLOGICA SINICA. DOI: 10.7541/2025.2025.0016
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In order to investigate the effect of sodium bicarbonate (NaHCO3) on intestinal damage in largemouth bass (Micropterus salmoides) under chronic high-temperature stress, juvenile largemouth bass (20.26±0.08) g were fed diets with or without sodium bicarbonate (NaHCO3, 5 g/kg) for 8 weeks. The results showed that there was no significant difference in the specific growth rate in the NaHCO3 group compared to the control group at chronic high temperatures. However, the NaHCO3 group exhibited significantly improved feed efficiency, protein deposition rate, and protein efficiency, along with a marked increase in intestinal amylase and trypsin activities. The addition of NaHCO3 also significantly enhanced the intestinal total antioxidant capacity (T-AOC), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities. Furthermore, NaHCO3 up-regulated the expression of cat and gpx1a genes and down-regulated the gene expression of keap1. Meanwhile, the expressions of pro-inflammatory cytokines (il-1β, tnfα, il15) were significantly down-regulated in the group, while the expression of anti-inflammatory cytokine il10 was up-regulated. In addition, the expression of hsp90, hsp70, and hsp60 genes in the intestines of largemouth bass in the NaHCO3 group was significantly lower than that in the control group. Histologic analysis of the intestinal tract showed that high-temperature stress induced intestinal damage in largemouth bass, and the addition of NaHCO3 significantly improved the intestinal tissue morphology and up-regulated the expression of intestinal zo1, jam, occludin, and muc2. In conclusion, the addition of 5 g/kg NaHCO3 to the feed effectively alleviated chronic high-temperature stress-induced intestinal damage in largemouth bass by improving intestinal antioxidant capacity, intestinal inflammation, and intestinal barrier function. These findings may provide a new perspective to improve the intestinal health of fish under high-temperature stress.
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